سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Kadivar, Maliheh, Sangsari, Razieh, Zarkesh, Mohammad Reza, Ashrafi, Mahmoud Reza, Shamel, Shirin, Mirnia, Kayvan, Saeedi, Maryam, Rosmati, Parastoo. (1403). Evaluation of Cerebrospinal Fluid and Plasma Amino Acids Levels in Neonates with Refractory Seizures: A Prospective Cohort Study. , 15(2), 28-34. doi: 10.22038/ijn.2024.71069.2384
Maliheh Kadivar; Razieh Sangsari; Mohammad Reza Zarkesh; Mahmoud Reza Ashrafi; Shirin Shamel; Kayvan Mirnia; Maryam Saeedi; Parastoo Rosmati. "Evaluation of Cerebrospinal Fluid and Plasma Amino Acids Levels in Neonates with Refractory Seizures: A Prospective Cohort Study". , 15, 2, 1403, 28-34. doi: 10.22038/ijn.2024.71069.2384
Kadivar, Maliheh, Sangsari, Razieh, Zarkesh, Mohammad Reza, Ashrafi, Mahmoud Reza, Shamel, Shirin, Mirnia, Kayvan, Saeedi, Maryam, Rosmati, Parastoo. (1403). 'Evaluation of Cerebrospinal Fluid and Plasma Amino Acids Levels in Neonates with Refractory Seizures: A Prospective Cohort Study', , 15(2), pp. 28-34. doi: 10.22038/ijn.2024.71069.2384
Kadivar, Maliheh, Sangsari, Razieh, Zarkesh, Mohammad Reza, Ashrafi, Mahmoud Reza, Shamel, Shirin, Mirnia, Kayvan, Saeedi, Maryam, Rosmati, Parastoo. Evaluation of Cerebrospinal Fluid and Plasma Amino Acids Levels in Neonates with Refractory Seizures: A Prospective Cohort Study. , 1403; 15(2): 28-34. doi: 10.22038/ijn.2024.71069.2384

Evaluation of Cerebrospinal Fluid and Plasma Amino Acids Levels in Neonates with Refractory Seizures: A Prospective Cohort Study

Iranian Journal of Neonatology
دوره 15، شماره 2، تیر 2024، صفحه 28-34    اصل مقاله (391.52 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijn.2024.71069.2384
نویسندگان
Maliheh Kadivar1؛ Razieh Sangsari1؛ Mohammad Reza Zarkesh2؛ Mahmoud Reza Ashrafi3؛ Shirin Shamel* 2؛ Kayvan Mirnia1؛ Maryam Saeedi1؛ Parastoo Rosmati4
1Department of Pediatrics, Division of Neonatology, School of Medicine, Tehran University of Medical Sciences, Children’s Medical Center, Tehran, Iran
22. Maternal, Fetal, and Neonatal Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran 3. Department of Neonatology, Yas Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
34. Department of Pediatric Neurology, Growth and Development Research Center, Children's Medical Center, Pediatrics Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran
4Department of Pediatrics, Division of Endocrinology and Metabolism, Growth and Development Research Center, Faculty of Medicine, Tehran University of Medical Sciences, Children’s Medical Center, Tehran, Iran
چکیده
Background: Altered concentrations of cerebrospinal fluid (CSF) and blood amino acids may be related to epilepsy or the severity of the seizure. In the present study, we assessed the concentrations of amino acids in CSF and plasma in neonates with refractory seizures.
Methods: In this prospective cohort study, 27 neonates aged 1 to 56 days with refractory seizures were included. Blood and CSF samples were collected from each neonate within 24 hours after receiving 2nd antiseizure medications. All plasma and CSF samples were sent to the laboratory to measure amino acid concentrations. The associations between CSF and plasma amino acid levels with different variables were evaluated.
Results: Except for leucine (P=0.15) and isoleucine (P=0.07), the levels of all amino acids were significantly higher in plasma than CSF. Significant associations were observed between types of seizure and plasma citrulline (P=0.008) and leucine concentrations (P=0.04). The mean of CSF glutamic acid was also statistically different among neonates with different EEG results (P=0.02).
Conclusion: Our findings indicate that several plasma and CSF amino acids could be candidate biomarkers for neonatal refractory seizures. Further studies with larger sample size are to confirm our findings.
 
 
کلیدواژه‌ها
Amino acid؛ Cerebrospinal fluid؛ Plasma؛ Seizure
مراجع
  1. Pisani F, Spagnoli C, Falsaperla R, Nagarajan L, Ramantani G. Seizures in the neonate: A review of etiologies and outcomes. Seizure. 2021;85:48-56.
  2. Donovan MD, Griffin BT, Kharoshankaya L, Cryan JF, Boylan GB. Pharmacotherapy for neonatal seizures: current knowledge and future perspectives. Drugs. 2016;76(6):647-661.
  3. Glass HC, Wirrell E. Controversies in neonatal seizure management. J Child Neurol. 2009; 24(5):591-599.
  4. Jones CM, Smith M, Henderson MJ. Reference data for cerebrospinal fluid and the utility of amino acid measurement for the diagnosis of inborn errors of metabolism. Ann Clin Biochem. 2006;43(Pt 1):63-66.
  5. Raymond LA, Blackstone CD, Huganir RL. Phosphorylation of amino acid neurotransmitter receptors in synaptic plasticity. Trends Neurosci. 1993;16(4):147-53.
  6. Saleem TH, Nassar AY, El-Tallawy HN, Atta SA, Dahpy MA. Role of plasma amino acids profiles in pathogenesis and prediction of severity in patients with drug resistant epilepsy. Egypt J Hosp Med. 2019;77(1):4681-4687.
  7. Rainesalo S, Keränen T, Palmio J, Peltola J, Oja SS, Saransaari P. Plasma and cerebrospinal fluid amino acids in epileptic patients. Neurochem Res. 2004;29(1):319-324.
  8. Wilson CL, Maidment NT, Shomer MH, Behnke EJ, Ackerson L, Fried I, et al. Comparison of seizure related amino acid release in human epileptic hippocampus versus a chronic, kainate rat model of hippocampal epilepsy. Epilepsy Res. 1996; 26(1):245-254.
  9. Ferrie C, Bird S, Tilling K, Maisey M, Chapman A, Robinson R. Plasma amino acids in childhood epileptic encephalopathies. Epilepsy Res. 1999; 34(2-3):221-229.
  10. Vossler DG, Bainbridge JL, Boggs JG, Novotny EJ, Loddenkemper T, Faught E, et al. Treatment of refractory convulsive status epilepticus: a comprehensive review by the American Epilepsy Society Treatments Committee. Epilepsy Curr. 2020;20(5):245-264.
  11. Lüders H, Acharya J, Baumgartner C, Benbadis S, Bleasel A, Burgess R, et al. Semiological seizure classification. Epilepsia. 1998;39(9):1006-1013.
  12. Noachtar S, Peters AS. Semiology of epileptic seizures: a critical review. Epilepsy Behav. 2009;15(1):2-9.
  13. Westmark CJ. A role for amino acid balance in dietary treatments for epilepsy. J Nutr. 2018;148(3):307-308.
  14. Gruenbaum SE, Dhaher R, Rapuano A, Zaveri HP, Tang A, de Lanerolle N, et al. Effects of branched-chain amino acid supplementation on spontaneous seizures and neuronal viability in a model of mesial temporal lobe epilepsy. J Neurosurg Anesthesiol. 2019;31(2):247.
  15. Czapinski P, Blaszczyk B, Czuczwar SJ. Mechanisms of action of antiepileptic drugs. Curr Top Med Chem. 2005;5(1):3-14.
  16. Dahlin M, Elfving Å, Ungerstedt U, Åmark P. The ketogenic diet influences the levels of excitatory and inhibitory amino acids in the CSF in children with refractory epilepsy. Epilepsy Res. 2005;64(3):115-25.
  17. Eghbalian F, Rasuli B, Monsef F. Frequency, causes, and findings of brain CT scans of neonatal seizure at Besat hospital, Hamadan, Iran. Iran J Child Neurol. 2015;9(1):56-63.
  18. Malik AR, Quddusi AI. Neonatal seizures, experience at Children Hospital and Institute of Child Health Multan. Pak J Med Sci. 2013;29(5):1128-1131.
  19. Kohelet D, Shochat R, Lusky A, Reichman B. Risk factors for neonatal seizures in very low birthweight infants: population-based survey. J Child Neurol. 2004;19(2):123-128.
  20. Blumcke I, Spreafico R, Haaker G, Coras R, Kobow K, Bien CG, et al. Histopathological findings in brain tissue obtained during epilepsy surgery. N Engl J Med. 2017;377(17):1648-1656.
  21. Vetrikani R, Bobby TC. Diagnosis of epilepsy—A systematic review. In 2017 Third International Conference on Biosignals, Images and Instrumentation (ICBSII). 2017:1-5. IEEE.
  22. Smith SJ. EEG in the diagnosis, classification, and management of patients with epilepsy. J Neurol Neurosurg Psychiatry. 2005;76 Suppl 2(Suppl 2):ii2-ii7.
  23. Toet MC, Groenendaal F, Osredkar D, van Huffelen AC, de Vries LS. Postneonatal epilepsy following amplitude-integrated EEG-detected neonatal seizures. Pediatr Neurol. 2005;32(4):241-247.
  24. Haider HA, Esteller R, Hahn CD, Westover MB, Halford JJ, Lee JW, et al. Sensitivity of quantitative EEG for seizure identification in the intensive care unit. Neurology. 2016;87(9):935-944.
  25. Swaiman KF, Ashwal S, Ferriero DM. Pediatric neurology: principles & practice. Elsevier Health Sciences. 2006.
  26. Farhadi R, Alaee A, Alipour Z, Abbaskhanian A, Nakhshab M, Derakhshanfar H. Prevalence of stroke in neonates who admitted with seizures in neonatal intensive care unit. Iran J Child Neurol. 2015;9(4):41-47.
  27. Fallah R, Yarhosseini Z, Nafisi Moghadam R. Evaluation of brain CT scan findings of neonates with clinical seizure admitted to Yazd Shahid Sadoughi Hospital and its correlation with clinical findings. SSU_Journals. 2018;26(4):294-305.
  28. Sabzehei MK, Basiri B, Bazmamoun H. The etiology, clinical type, and short outcome of seizures in newbornshospitalized in Besat Hospital/ Hamadan/Iran. Iran J Child Neurol. 2014;8(2):24-28.
  29. Origuchi Y, Ushijima T, Sakaguchi M, Akaboshi I, Matsuda I. Citrullinemia presenting as uncontrollable epilepsy. Brain Dev. 1984;6(3):328-331.
  30. Zhang Y, Yan-ling C, HAO M-m, Na Q, Yan-chun D. Clinical significance of elevated serum citrulline levels in patients with epilepsy. Chin J Contemp Neurol Neurosurg. 2014;14(11):990.
  31. Pu Y, Garg A, Corby R, Gao J-H, Zeng C-M, Li Q-F. A positive correlation between α-glutamate and glutamine on brain 1H-MR spectroscopy and neonatal seizures in moderate and severe hypoxic-ischemic encephalopathy. AJNR Am J Neuroradiol. 2008;29(2):216.
  32. Zhou Y, Danbolt NC. Glutamate as a neurotransmitter in the healthy brain. J Neural Transm (Vienna). 2014;121(8):799-817.
  33. Jensen FE. The role of glutamate receptor maturation in perinatal seizures and brain injury. Int J Dev Neurosci. 2002;20(3-5):339-347.
  34. Sharma S, Prasad AN. Inborn errors of metabolism and epilepsy: current understanding, diagnosis, and treatment approaches. Int J Mol Sci. 2017; 18(7):1384.
آمار
تعداد مشاهده مقاله: 236
تعداد دریافت فایل اصل مقاله: 252