Evaluation of the influence of N-Acetylcysteine and broccoli extract on systemic paraquat poisoning: Implications for biochemical, physiological, and histopathological parameters in rats | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 13، دوره 27، شماره 7، مهر 2024، صفحه 895-903 اصل مقاله (544.21 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.75258.16311 | ||
| نویسندگان | ||
| Mahdieh Raeeszadeh* 1؛ Sara Arvand2؛ Danial Shojaee Moghadam2؛ Loghman Akradi3 | ||
| 1Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran | ||
| 2Graduate of Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran | ||
| 3Department of Pathobiology Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran | ||
| چکیده | ||
| Objective(s): Paraquat (PQ), a potent environmental herbicide, is recognized for inducing irreparable toxic damage to biological systems. This study aimed to evaluate the effectiveness of N-acetylcysteine (NAC) and broccoli extract, individually and in combination, in alleviating PQ poisoning in rats, leveraging the exceptional anti-oxidant, anti-inflammatory, and anti-apoptotic properties of broccoli. Materials and Methods: Seventy Wistar rats were categorized into seven groups: C (control, vehicle), PQ (paraquat at 40 mg/kg), BC (broccoli extract at 300 mg/kg), NC (N-acetylcysteine at the same dose of 300 mg/kg), and combined groups PQ+BC, PQ+NC, and NC+PQ+BC, all administered equivalent doses. After 42 days, blood samples were collected to evaluate liver and kidney parameters, proinflammatory biomarkers, caspase-3, and caspase-9. Lung tissues were excised, with one part preserved for hydroxyproline and oxidative stress parameter measurement and another sectioned and stained for histopathological analysis. Results: The PQ group exhibited the highest lung-to-body weight (LW/BW) ratio, while the PQ+BC+NC group demonstrated the lowest ratio. Results indicated an elevated lung hydroxyproline concentration and a significant reduction in anti-oxidant enzymes (catalase, glutathione peroxidase, superoxide dismutase, and total anti-oxidant capacity) (P<0.001). The PQ+BC group showed modified malondialdehyde levels, reaching a peak in the PQ group. Additionally, a significant decrease in tumor necrosis factor, interleukin-1, caspase-3, and caspase-9 was observed in the PQ+BC+NC group (P<0.01). Pulmonary edema, hyperemia, and severe hemorrhage observed in the PQ group were notably reduced in the PQ+BC+NC group. Conclusion: The combination of active compounds from broccoli and NAC demonstrated significant systemic and pulmonary effects in mitigating PQ-induced toxicity. | ||
| کلیدواژهها | ||
| Apoptosis؛ Broccoli؛ N-acetyl cysteine؛ Oxidative stress؛ Paraquat | ||
| مراجع | ||
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