سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Yazdanparast, Somayeh, Bashash, Davood, Nikkhah Bahrami, Amirsalar, Ghorbani, Mohammad, Izadirad, Mehrdad, Bakhtiyaridovvombaygi, Mehdi, Gharehbaghian, Ahmad. (1403). Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL. , 27(7), 801-812. doi: 10.22038/ijbms.2024.76261.16498
Somayeh Yazdanparast; Davood Bashash; Amirsalar Nikkhah Bahrami; Mohammad Ghorbani; Mehrdad Izadirad; Mehdi Bakhtiyaridovvombaygi; Ahmad Gharehbaghian. "Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL". , 27, 7, 1403, 801-812. doi: 10.22038/ijbms.2024.76261.16498
Yazdanparast, Somayeh, Bashash, Davood, Nikkhah Bahrami, Amirsalar, Ghorbani, Mohammad, Izadirad, Mehrdad, Bakhtiyaridovvombaygi, Mehdi, Gharehbaghian, Ahmad. (1403). 'Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL', , 27(7), pp. 801-812. doi: 10.22038/ijbms.2024.76261.16498
Yazdanparast, Somayeh, Bashash, Davood, Nikkhah Bahrami, Amirsalar, Ghorbani, Mohammad, Izadirad, Mehrdad, Bakhtiyaridovvombaygi, Mehdi, Gharehbaghian, Ahmad. Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL. , 1403; 27(7): 801-812. doi: 10.22038/ijbms.2024.76261.16498

Royal jelly induces ROS-mediated apoptosis in acute lymphoblastic leukemia (ALL)-derived Nalm-6 cells: Shedding light on novel therapeutic approaches for ALL

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 27، شماره 7، مهر 2024، صفحه 801-812    اصل مقاله (1.23 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2024.76261.16498
نویسندگان
Somayeh Yazdanparast1؛ Davood Bashash2؛ Amirsalar Nikkhah Bahrami3؛ Mohammad Ghorbani4، 5؛ Mehrdad Izadirad2؛ Mehdi Bakhtiyaridovvombaygi2؛ Ahmad Gharehbaghian* 2، 6
1Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Laboratory Hematology and Blood Bank Department, School of Allied Medical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
4Laboratory Hematology and Transfusion Medicine, Department of Pathology, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
5Laboratory Hematology and Transfusion Medicine, Department of Medical Laboratory Sciences, Faculty of Allied Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
6Pediatric Congenital Hematologic Disorders Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Until recently, a conventional chemotherapy regimen for Acute lymphoblastic leukemia (ALL) is considered an efficient therapeutic method in children. However, suboptimal long-term survival rates in adults, disease relapse, and drug-induced toxicities require novel therapeutic agents for ALL treatments. Today, natural products with pharmacological benefits play a significant role in treating different cancers. Among the most valued natural products, honey bees’ royal jelly (RJ) is one of the most appreciated which has revealed anti-tumor activity against different human cancers. This study aimed to evaluate anti-leukemic properties and the molecular mechanisms of RJ cytotoxicity on ALL-derived Nalm-6 cells.
Materials and Methods: The metabolic activity was measured by MTT assay. Apoptosis, cell distribution in the cell cycle, and intracellular reactive oxygen species (ROS) level were investigated using flow cytometry analysis. Moreover, quantitative real-time PCR (qRT-PCR) was performed to scrutinize the expression of various regulatory genes. 
Results: RJ significantly decreased the viability of Nalm-6 cells but had no cytotoxic effect on normal cells. In addition, RJ induced ROS-mediated apoptosis by up-regulating pro-apoptotic genes while decreasing anti-apoptotic gene expression. The results outlined that ROS-dependent up-regulation of FOXO4 and Sirt1 inhibits the cells’ transition to the S phase of the cell cycle through p21 up-regulation. The qRT-PCR analysis of autophagy-related gene expression also demonstrated that RJ induced BECN1 mediated autophagy in Naml-6 cells.
Conclusion: Taken together, this study showed that RJ can be utilized as a potent natural substance to induce ALL cells’ programmed cell death. However, further studies are required to examine this compound’s pharmaceutical application.  
کلیدواژه‌ها
Acute lymphoblastic - leukemia (ALL)؛ Apoptosis؛ Nalm-6؛ Reactive oxygen species - (ROS)؛ Royal jelly (RJ)
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