Potential antiaging activity of secretome gel of human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 27، شماره 7، مهر 2024، صفحه 868-878 اصل مقاله (1.08 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.70825.15385 | ||
| نویسندگان | ||
| Wahyu Widowati* 1؛ Ahmad Faried2، 3؛ Achmad Adam2، 3؛ Deni Rahmat4؛ Hanna Sari Widya Kusuma5؛ Nindia Salsabila Mia Dewi5؛ Marisca Gondokesumo6؛ Rizal Rizal5، 7؛ Ita Margaretha Nainggolan8، 9؛ Massoud Vosough10، 11 | ||
| 1Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia | ||
| 2Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia | ||
| 3Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia | ||
| 4Faculty of Pharmacy, Pancasila University, South Jakarta, Indonesia | ||
| 5Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia | ||
| 6Faculty of Pharmacy, University of Surabaya, Universitas Surabaya, Surabaya, East Java, Indonesia | ||
| 7Biomedical Engineering Department of Electrical Engineering, Faculty of Engineering University of Indonesia, Jakarta, Indonesia | ||
| 8Clinical Pathology Department, School of Medicine and Health Sciences, Atma Jaya Catholic University, Jakarta, Indonesia | ||
| 9Eijkman Research Center for Molecular Biology, National Research and Innovation Agency, Bogor, West Java, Indonesia | ||
| 10Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran | ||
| 11Experimental Cancer Medicine, Institution for Laboratory Medicine, Karolinska, Institute, Stockholm, Sweden | ||
| چکیده | ||
| Objective(s): Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model. Materials and Methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice’s dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (P<0.05). Results: UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1. Conclusion: The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging. | ||
| کلیدواژهها | ||
| Inflammation؛ Mesenchymal stem cells؛ Oxidative stress؛ Secretome؛ Skin aging | ||
| مراجع | ||
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