Protective effects of naringin against oxaliplatin-induced testicular damage in rats: Involvement of oxidative stress, inflammation, endoplasmic reticulum stress, apoptosis, and histopathology | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 9، دوره 27، شماره 4، تیر 2024، صفحه 466-474 اصل مقاله (948.39 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2024.73824.16048 | ||
| نویسندگان | ||
| Nurhan Akaras* 1؛ Cihan Gur2؛ Cuneyt Caglayan3؛ Fatih Mehmet Kandemir4 | ||
| 1Department of Histology and Embryology, Faculty of Medicine, Aksaray University, Aksaray, Turkey | ||
| 2Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey | ||
| 3Department of Medical Biochemistry, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, Turkey | ||
| 4Department of Medical Biochemistry, Faculty of Medicine, Aksaray University, Aksaray, Turkey | ||
| چکیده | ||
| Objective(s): Oxaliplatin (OXL) is a platinum-based chemotherapeutic agent widely used in the treatment of colorectal cancer. Unfortunately, this important drug also causes unwanted side effects such as neuropathy, ototoxicity, and testicular toxicity. This study aimed to investigate the possible protective effects of naringin (NRG) against OXL-induced testicular toxicity in rats. Materials and Methods: In the present study, rats were injected with OXL (4 mg/kg, b.w./day, IP) in 5% dextrose solution 30 min after oral administration of NRG (50 and 100 mg/kg, b.w./day) on the 1st, 2nd, 5th, and 6th days. Then, the rats were sacrificed on the 7th day and the testicular tissues were removed. Results: The results showed that NRG decreased (P<0.001) lipid peroxidation, increased (P<0.001) the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and the levels of glutathione (GSH), and also maintained the testis histological architecture and integrity. NRG decreased the levels of apoptosis-related markers such as caspase-3, Bax, and Apaf-1 and increased Bcl2 in the OXL-induced testicular toxicity (P<0.001). In addition, NRG reversed the changes in mRNA transcript levels of oxidative stress, inflammation, and endoplasmic reticulum stress parameters such as Nrf2, HO-1, NQO1, RAGE, NLRP3, MAPK-14, STAT3, NF-κB, IL-1β, TNF-α, PERK, IRE1, ATF6, and GRP78 in OXL-induced testicular toxicity (P<0.001). Conclusion: Our results demonstrated that NRG can protect against OXL-induced testicular toxicity by enhancing the anti-oxidant defense system and suppressing apoptosis, inflammation, and endoplasmic reticulum stress. | ||
| کلیدواژهها | ||
| Apoptosis؛ Endoplasmic reticulum - stress؛ Inflammation؛ Naringin؛ Oxaliplatin؛ Testicular toxicity | ||
| مراجع | ||
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