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Asoudeh-Fard, Abbas, Salehi, Mitra, Ilghari, Dariush, Parsaei, Asghar, Heydarian, Peyman, Piri, Hossein. (1403). Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway. , 27(4), 447-452. doi: 10.22038/ijbms.2023.72825.15846
Abbas Asoudeh-Fard; Mitra Salehi; Dariush Ilghari; Asghar Parsaei; Peyman Heydarian; Hossein Piri. "Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway". , 27, 4, 1403, 447-452. doi: 10.22038/ijbms.2023.72825.15846
Asoudeh-Fard, Abbas, Salehi, Mitra, Ilghari, Dariush, Parsaei, Asghar, Heydarian, Peyman, Piri, Hossein. (1403). 'Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway', , 27(4), pp. 447-452. doi: 10.22038/ijbms.2023.72825.15846
Asoudeh-Fard, Abbas, Salehi, Mitra, Ilghari, Dariush, Parsaei, Asghar, Heydarian, Peyman, Piri, Hossein. Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway. , 1403; 27(4): 447-452. doi: 10.22038/ijbms.2023.72825.15846

Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 27، شماره 4، تیر 2024، صفحه 447-452    اصل مقاله (405.75 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.72825.15846
نویسندگان
Abbas Asoudeh-Fard1، 2؛ Mitra Salehi3، 4؛ Dariush Ilghari5؛ Asghar Parsaei6؛ Peyman Heydarian7، 8؛ Hossein Piri* 7، 9
1INSERM U1148, Laboratory for Vascular Translation Science (LVTS), Cardiovascular Bioengineering, University Sorbonne Paris North, Paris, France
2Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
3Clinical Research Development Unit, Booalisina Hospital, Qazvin University of Medical Sciences, Qazvin, Iran
4Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
5Clinical Pharmacist, Baylor Scott & White Medical Center – Lakeway 100 Medical Pkwy, Lakeway, TX 78738
6Rayan Novin Pajoohan Pras, Biotechnology Company, Biotechnology Incubator, Shiraz University of Medical Sciences, Shiraz, Iran
7Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
8Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
9Department of Biochemistry and Genetics, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
چکیده
Objective(s): It is worthwhile to note that, some probiotics such as Lactobacilli and Bifidobacteria isolated from dairy products have significant therapeutic effects against cancer cells. Here, we evaluated anti-proliferation and the apoptotic effects of isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products on the HeLa cervical cancer cells in vitro.
Materials and Methods: The viability of treated HeLa cells with supernatant of Lactobacillus in 0.5, 0.75, 1, 1.5, and 2 ng/ml concentrations, and IC50 values were detected by tetrazolium bromide. The L. fermentum Ab.RS22-induced cell death by flow cytometry was confirmed through evaluation of the expression of caspase-3, P53, PTEN, and AKT genes by quantitative reverse transcription-polymerase chain reactions (qRT-PCR).
Results: Most cytotoxicity effects of Lactobacillus on HeLa cells were detected in 2 ng/ml at 24 hr (P<0.01); also, the IC50 value was measured as 1.5 ng/ml. The findings of the flow cytometry assay showed that L. fermentum Ab.RS22 in 1.5 ng/ml concentration at 24 hr increased the percentage of both apoptosis and necrosis cells. Lactobacillus-induced cell death was verified through results of Real-time PCR; where expression of caspase-3, P53, and PTEN genes was increased (P<0.01), and also expression of AKT gene (anti-apoptotic) was decreased (P<0.05). 
Conclusion: Our findings showed that L. fermentum Ab.RS22 could dose-dependently inhibit the proliferation of the HeLa cells. Its apoptotic effect was confirmed via modulating PTEN/p53/Akt gene expression and activation of the caspase-3 mediated apoptosis pathway. Therefore, L. fermentum Ab.RS22 can be considered a valuable anticancer candidate against cervical cancer progression in subsequent studies. 
کلیدواژه‌ها
Apoptosis؛ Lactobacillus؛ Microbiology؛ Probiotics؛ PTEN protein؛ Tumor suppressor protein p53
مراجع
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