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Wang, Guiying, Deng, Jiuhong, Hua, Zhou. (1403). Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway. , 27(4), 439-446. doi: 10.22038/ijbms.2023.73385.15942
Guiying Wang; Jiuhong Deng; Zhou Hua. "Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway". , 27, 4, 1403, 439-446. doi: 10.22038/ijbms.2023.73385.15942
Wang, Guiying, Deng, Jiuhong, Hua, Zhou. (1403). 'Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway', , 27(4), pp. 439-446. doi: 10.22038/ijbms.2023.73385.15942
Wang, Guiying, Deng, Jiuhong, Hua, Zhou. Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway. , 1403; 27(4): 439-446. doi: 10.22038/ijbms.2023.73385.15942

Crocin protects against endoplasmic reticulum stress-related tubular injury in diabetic nephropathy via the activation of the PI3K/AKT/Nrf2 pathway

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 27، شماره 4، تیر 2024، صفحه 439-446    اصل مقاله (894.21 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.73385.15942
نویسندگان
Guiying Wang1؛ Jiuhong Deng2؛ Zhou Hua* 3
1Department of Nephrology, Shangyu People’s Hospital of Shaoxing, Shaoxing, China
2Department of Endocrinology, Second People’s Hospital of Pingyang County, Wenzhou, China
3Department of Nephrology, The People’s Hospital of Suichang County, Lishui, China
چکیده
Objective(s): Diabetic nephropathy (DN) is the main cause of end-stage renal disease, but the current treatment is not satisfactory. Crocin is a major bioactive compound of saffron with antioxidant and anti-endoplasmic reticulum stress (ERS) abilities used to treat diabetes. This study specifically investigated whether crocin has a regulatory role in renal injury in DN.
Materials and Methods: The experiment was divided into control, (db/m mice), model (db/db mice), and experimental groups (db/db mice were intraperitoneally injected with 40 mg/kg crocin). Renal function-related indicators (Scr, BUN, FBG, UP, TG, TC, ALT, and AST) and oxidative stress-related indicators (ROS, MDA, GSH, SOD, and CAT) were assessed. The pathological changes of renal tissues were confirmed by HE, Masson, PAS, and TUNEL staining. The levels of ERS-related proteins (GRP78 and CHOP), apoptosis-related proteins, and PI3K/AKT and Nrf2 pathways-related proteins in renal tissue were detected. 
Results: In db/db mice, renal function-related indicators, apoptotic cells of renal tissues, the contents of ROS and MDA as well as the expressions of CHOP, GRP78, and Bax were increased, the degree of renal tissue damage was aggravated, while the contents of GSH, SOD, and CAT, as well as the protein levels of Nrf2, PARP, anti-apoptotic proteins (Mcl-1, Bcl-2, Bcl-xl) were decreased compared to the db/m mice. However, crocin treatment reversed the above-mentioned situation. The expressions of the PI3K/AKT and Nrf2 pathways-related proteins were also activated by crocin.
Conclusion: Crocin inhibited oxidative stress and ERS-induced kidney injury in db/db mice by activating the PI3K/AKT and Nrf2 pathways.
کلیدواژه‌ها
Crocin؛ Diabetic nephropathy؛ Endoplasmic reticulum - stress؛ Kidney injury؛ PI3K/AKT
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