سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Liu, Zhanli, Xu, Jiqing, Zhao, Yanqiu, Wan, Yanbin, Guo, Rui, Long, Canling, Liu, Jia, Yao, Xinhuang, Yin, Wenchao. (1403). Dexmedetomidine protects against sepsis-induced lung injury through autophagy and Smad2/3 signaling pathway. , 27(4), 453-460. doi: 10.22038/ijbms.2023.73479.15964
Zhanli Liu; Jiqing Xu; Yanqiu Zhao; Yanbin Wan; Rui Guo; Canling Long; Jia Liu; Xinhuang Yao; Wenchao Yin. "Dexmedetomidine protects against sepsis-induced lung injury through autophagy and Smad2/3 signaling pathway". , 27, 4, 1403, 453-460. doi: 10.22038/ijbms.2023.73479.15964
Liu, Zhanli, Xu, Jiqing, Zhao, Yanqiu, Wan, Yanbin, Guo, Rui, Long, Canling, Liu, Jia, Yao, Xinhuang, Yin, Wenchao. (1403). 'Dexmedetomidine protects against sepsis-induced lung injury through autophagy and Smad2/3 signaling pathway', , 27(4), pp. 453-460. doi: 10.22038/ijbms.2023.73479.15964
Liu, Zhanli, Xu, Jiqing, Zhao, Yanqiu, Wan, Yanbin, Guo, Rui, Long, Canling, Liu, Jia, Yao, Xinhuang, Yin, Wenchao. Dexmedetomidine protects against sepsis-induced lung injury through autophagy and Smad2/3 signaling pathway. , 1403; 27(4): 453-460. doi: 10.22038/ijbms.2023.73479.15964

Dexmedetomidine protects against sepsis-induced lung injury through autophagy and Smad2/3 signaling pathway

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 27، شماره 4، تیر 2024، صفحه 453-460    اصل مقاله (782.38 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.73479.15964
نویسندگان
Zhanli Liu1؛ Jiqing Xu2؛ Yanqiu Zhao3؛ Yanbin Wan3؛ Rui Guo3؛ Canling Long3؛ Jia Liu3؛ Xinhuang Yao* 3؛ Wenchao Yin* 4
1Department of Anesthesiology, Shenzhen People’s Hospital and Shenzhen Anesthesiology Engineering Center, the Second Clinical Medical College of Jinan University, Shenzhen, China
2Department of Cardiothoracic Surgery, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of Shenzhen, Shenzhen, China
3Central Laboratory, The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People’s Hospital of Shenzhen, Shenzhen, China
4Department of Anesthesiology, Sichuan Provincial Orthopedic Hospital, Chengdu, China
چکیده
Objective(s): Dexmedetomidine (Dex) is a potent α2-adrenergic receptor(α2-AR) agonist that has been shown to protect against sepsis-induced lung injury, however, the underlying mechanisms of this protection are not fully understood. Autophagy and the Smad2/3 signaling pathway play important roles in sepsis-induced lung injury, but the relationship between Dex and Smad2/3 is not clear. This study aimed to investigate the role of autophagy and the Smad2/3 signaling pathway in Dex-mediated treatment of sepsis-induced lung injury. Sepsis was performed using cecal ligation and puncture (CLP) in C57BL/6J mice. 
Materials and Methods: Mice were randomly assigned to four groups (n=6 per group): sham, CLP, CLP-Dex, and CLP-Dex-YOH, Yohimbine hydrochloride (YOH) is an α2-AR blocker. The cecum was carefully separated to avoid blood vessel damage and was identified and punctured twice with an 18-gauge needle. The pathological changes, inflammatory factor levels, oxidative stress, autophagy, Smad2/3 signaling pathway-related protein levels in lung tissues, and the activity of superoxide dismutase (SOD) and malonaldehyde (MDA) in the serum were measured.
Results: CLP-induced lung injury was reflected by increased levels of inflammatory cytokines, apoptosis, and oxidative stress, along with an increase in the expression of autophagy and Smad2/3 signaling pathway-related proteins. Dex could reverse these changes and confer a protective effect on the lung during sepsis. However, the administration of YOH significantly reduced the positive effects of Dex in mice with sepsis.
Conclusion: Dex exerts its beneficial effects against sepsis-induced lung injury through the regulation of autophagy and the Smad2/3 signaling pathway.
کلیدواژه‌ها
Acute lung injury؛ Autophagy؛ Dexmedetomidine؛ Sepsis؛ Smad2/3
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