Salidroside alleviates LPS-induced liver injury and inflammation through SIRT1- NF-κB pathway and NLRP3 inflammasome | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 5، دوره 27، شماره 3، خرداد 2024، صفحه 297-303 اصل مقاله (889.55 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.69401.15124 | ||
| نویسندگان | ||
| Jialei Meng؛ Yunfeng Li؛ Fangyuan Sun؛ Wentao Feng؛ Hui Ye؛ Tianning Tian؛ Ming Lei* | ||
| Trauma Emergency Center, Seventh People’s Hospital of Shanghai University of Traditional Chinese Medicine, No.358, Datong Road, Pudong New District, Shanghai 200137, China | ||
| چکیده | ||
| Objective(s): Salidroside (SAL), an active ingredient purified from the medicinal plant Rhodiola rosea, has anti-inflammatory, anti-oxidant, anticancer, and neuroprotective properties. The study aims to examine SAL’s protective role in liver damage brought on by lipopolysaccharide (LPS). Materials and Methods: Six to eight-week-old male C57BL/6 wild-type mice were intraperitoneally treated with 10 mg/kg LPS for 24 hr and 50 mg/kg SAL two hours before LPS administration. Mice were categorized into control, LPS, and LPS + SAL groups. To evaluate liver injury, biochemical and TUNNEL staining test studies were performed. The Elisa assay analyzed interleukin- 1β (IL-1β), tumor necrosis factor-alpha (TNF-α), and interleukin-6 (IL-6) pro-inflammatory cytokine expression levels. RT-qPCR and western blotting measured mRNA and protein expression of SIRT1, NF-кB, NLRP3, cleaved caspase-1, and GSDMD, respectively. Results: Analysis of the serum alanine/aspartate aminotransferases (ALT/AST), malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) revealed that SAL protected against hepatotoxicity induced by LPS. The pathological evaluation of the liver supported the protection provided by SAL. SAL treatment reversed IL-1β, TNF-α, and IL-6 pro-inflammatory cytokines after being induced by LPS (all, P<0.001). The western blotting examination results demonstrated that SAL increased the levels of Sirtuin 1 (SIRT1) expression but markedly reduced the phosphorylation of Nuclear Factor Kappa B (NF-B) and the expressions of NLRP3, cleaved caspase-1, and gasdermin D (GSDMD) induced by LPS (all, P<0.001). Conclusion: Our results speculated that by inhibiting the SIRT1- NF-κB pathway and NLRP3 inflammasome, SAL defends against LPS-induced liver injury and inflammation. | ||
| کلیدواژهها | ||
| Anti-inflammation؛ Lipopolysaccharide؛ Liver injury؛ Nuclear factor Kappa B؛ Salidroside | ||
| مراجع | ||
|
1. Hattori Y, Hattori K, Suzuki T, Matsuda N. Recent advances in the pathophysiology and molecular basis of sepsis‐associated organ dysfunction: Novel therapeutic implications and challenges. Pharmacol Ther 2017; 177: 56-66. 2. Jiang Z, Meng Y, Bo L, Wang C, Bian J, Deng X. Sophocarpine attenuates LPS‐induced liver injury and improves survival of mice through suppressing oxidative stress, inflammation, and apoptosis. Mediators Inflamm 2018; 2018: 5871431. 3. Guo FM, Qiu HB. Definition and diagnosis of sepsis 3.0. Zhonghua Nei Ke Za Zhi. 2016; 55: 420-422. 4. Woźnica EA, Inglot M, Woźnica RK, Łysenko L. Liver dysfunction in sepsis. Adv Clin Exp Med 2018; 27: 547-551. 5. Hu C, Li L. Improvement of mesenchymal stromal cells and their derivatives for treating acute liver failure. J Mol Med 2019; 97: 1065-1084. 6. Strnad P, Tacke F, Koch A, Trautwein C. Liver-guardian, modifier and target of sepsis. Nat Rev Gastroenterol Hepatol 2017; 14: 55-66. 7. Ingawale DK, Mandlik SK, Naik SR. Models of hepatotoxicity and the underlying cellular, biochemical and immunological mechanism(s): A critical discussion. Environ Toxicol Pharmacol 2014; 37: 118-133. 8. Gao LN, Yan K, Cui YL, Fan GW, Wang YF. Protective effect of Salvia miltiorrhiza and Carthamus tinctorius extract against lipopolysaccharide-induced liver injury. World J Gastroenterol 2015; 21: 9079-9092. 9. Qian EW, Ge DT, Kong SK. Salidroside protects human erythrocytes against hydrogen peroxide-induced apoptosis. J Nat Prod 2012; 75: 531-537. 10. Song B, Huang G, Xiong Y, Liu J, Xu L, Wang Z, et al. Inhibitory effects of salidroside on nitric oxide and prostaglandin E2 production in lipopolysaccharide-stimulated RAW 264.7 macrophages. J Med Food 2013; 16: 997-1003. 11. Zhang Y, Yao Y, Wang H, Guo Y, Zhang H, Chen L. Effects of salidroside on glioma formation and growth inhibition together with improvement of tumor microenvironment. Chin J Cancer Res 2013; 25: 520-526. 12. Zhu Y, Shi YP, Wu D, Ji YJ, Wang X, Chen HL, et al. Salidroside protects against hydrogen peroxide-induced injury in cardiac H9c2 cells via PI3K-Akt dependent pathway. DNA Cell Biol 2011; 30: 809-819. 13. Guan S, Xiong Y, Song B, Song Y, Wang D, Chu X, et al. Protective effects of salidroside from Rhodiola rosea on LPS-induced acute lung injury in mice. Immunopharmacol Immunotoxicol 2012; 34: 667-672. 14. Zhang Z, Ding L, Wu L, Xu L, Zheng L, Huang X. Salidroside alleviates paraquat-induced rat acute lung injury by repressing TGF-β1 expression. Int J Clin Exp Pathol 2014; 7: 8841-8847. 15. Lu R, Wu Y, Guo H, Huang X. Salidroside protects lipopolysaccharide-induced acute lung injury in mice. Dose-Response 2016; 14: 1559325816678492. 16. Lan KC, Chao SC, Wu HY, Chiang CL, Wang CC, Liu SH, et al. Salidroside ameliorates sepsis-induced acute lung injury and mortality via downregulating NF-κB and HMGB1 pathways through the upregulation of SIRT1. Sci Rep 2017; 7:12026-12036. 17. Lin SY, Xu D, Du XX, Ran CL, Xu L, Ren SJ, et al. Protective effects of salidroside against carbon tetrachloride (CCl4)-induced liver injury by initiating mitochondria to resist oxidative stress in mice. Int J Mol Sci 2019; 20: 3187-3200. 18. Yuan Y, Wu SJ, Liu X, Zhang LL. Anti-oxidant effect of salidroside and its protective effect against furan-induced hepatocyte damage in mice. Food Funct 2013; 4:763-769. 19. Zhang XR, Fu XJ, Zhu DS, Zhang CZ, Hou S, Li M, et al., Salidroside-regulated lipid metabolism with down-regulation of miR-370 in type 2 diabetic mice. Eur J Pharmacol 2016; 779: 46-52. 20. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C (T)) method. Methods 2001; 25: 402-408. 21. Li W, Yang S, Kim SO, Reid G, Challis JRG, Bocking AD. Lipopolysaccharide-induced profiles of cytokine, chemokine, and growth factors produced by human decidual cells are altered by lactobacillus rhamnosus GR-1 supernatant. Reprod Sci 2014; 21: 939-947. 22. Li D, Fu Y, Zhang W, Su G, Liu B, Guo M, et al., Salidroside attenuates inflammatory responses by suppressing nuclear factor-κB and mitogen activated protein kinases activation in lipopolysaccharide-induced mastitis in mice. Inflamm Res 2013; 62:9-15. 23. Schwabe RF, Luedde T. Apoptosis and necroptosis in the liver: A matter of life and death. Nat Rev Gastroenterol Hepatol 2018; 15: 738-752. 24. Kisseleva T, Brenner D. Molecular and cellular mechanisms of liver fibrosis and its regression. Nat Rev Gastroenterol Hepatol 2020; 18: 151-166. 25. Bock FJ, Tait SWG. Mitochondria as multifaceted regulators of cell death. Nat Rev Mol Cell Biol 2020; 21: 85-100. 26. Zhou J, Zhang J, Wang C, Qu S, Zhu Y, Yang Z, Wang L. Acai (Euterpe oleracea Mart.) attenuates alcohol-induced liver injury in rats by alleviating oxidative stress and inflammatory response. Exp Ther Med 2018; 15: 166-172. 27. Yan H, Huang Z, Bai Q, Sheng Y, Hao Z, Wang Z, et al. Natural product andrographolide alleviated APAP-induced liver fibrosis by activating Nrf2 anti-oxidant pathway. Toxicology 2018; 396-397: 1-12. 28. Zheng Z, Sheng Y, Lu B, Ji L. The therapeutic detoxification of chlorogenic acid against acetaminophen-induced liver injury by ameliorating hepatic inflammation. Chem Biol Interact 2015; 238: 93-101. 29. Ji L, Ma Y, Wang Z, Cai Z, Pang C,Wang Z. Quercetin prevents pyrrolizidine alkaloid clivorine-induced liver injury in mice by elevating body defense capacity. PLoS One 2014; 9: e98970. 30. Schoonbroodt S, Ferreira V, Best-Belpomme M, Boelaert JR, Legrand-Poels S, Korner M, et al. Crucial role of the amino-terminal tyrosine residue 42 and the carboxyl-terminal PEST domain of I kappa B alpha in NF-kappa B activation by an oxidative stress. J Immunol 2000; 164: 4292-300. 31. Zhang J, X. Wang, V. Vikash, Ye Q, Wu D, Liu Y, et al. ROS and ROS-mediated cellular signaling. Oxid Med Cell Longev 2016; 2016: 4350965. 32. Pang Y, Wu D, Ma Y, Cao Y, Liu Q, Tang M, et al. Reactive oxygen species trigger NF-κB-mediated NLRP3 inflammasome activation involvement in low-dose CdTe QDs exposure-induced hepatotoxicity. Redox Biology 2021; 47: 102157. | ||
|
آمار تعداد مشاهده مقاله: 993 تعداد دریافت فایل اصل مقاله: 802 |
||