سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Wang, Chun, Jiang, Chen, Yang, Yiran, Xi, Cheng, Yin, Yunxiang, Wu, Haiying, Qian, Chuanyun. (1402). Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury. , 27(3), 375-382. doi: 10.22038/ijbms.2023.74372.16156
Chun Wang; Chen Jiang; Yiran Yang; Cheng Xi; Yunxiang Yin; Haiying Wu; Chuanyun Qian. "Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury". , 27, 3, 1402, 375-382. doi: 10.22038/ijbms.2023.74372.16156
Wang, Chun, Jiang, Chen, Yang, Yiran, Xi, Cheng, Yin, Yunxiang, Wu, Haiying, Qian, Chuanyun. (1402). 'Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury', , 27(3), pp. 375-382. doi: 10.22038/ijbms.2023.74372.16156
Wang, Chun, Jiang, Chen, Yang, Yiran, Xi, Cheng, Yin, Yunxiang, Wu, Haiying, Qian, Chuanyun. Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury. , 1402; 27(3): 375-382. doi: 10.22038/ijbms.2023.74372.16156

Therapeutic potential of HUC-MSC-exos primed with IFN-γ against LPS-induced acute lung injury

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 27، شماره 3، خرداد 2024، صفحه 375-382    اصل مقاله (1.04 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.74372.16156
نویسندگان
Chun Wang1، 2؛ Chen Jiang1؛ Yiran Yang1؛ Cheng Xi3؛ Yunxiang Yin2؛ Haiying Wu4؛ Chuanyun Qian* 4
1Kunming Medical University, Kunming, China
2Department of Emergency Intensive Care Unit, Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650000, China
3Department of Gastrointestinal Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650000, China
4Department of Emergency, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650000, China
چکیده
Objective(s): Human umbilical cord mesenchymal stem cells (HUC-MSCs) are pluripotent stem cells with anti-inflammatory and immunomodulatory properties used in the treatment of acute lung injury (ALI). However, the treatment of ALI using exosomes derived from HUC-MSCs (HUC-MSC-exos) primed with interferon-gamma (IFN-γ-exos) has not been described. This study investigated the effects of IFN-γ-exos on ALI.
Materials and Methods: IFN-γ primed and unprimed HUC-MSC-exos (IFN-γ-exos and CON-exos, respectively) were extracted, identified, and traced. A549 cells and mice subjected to lipopolysaccharide (LPS)-induced inflammation were treated with IFN-γ-exos or CON-exos. Viability; interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and reactive oxygen species (ROS) levels; NF-κB p65, and NLRP3 expression and histology and lung injury scores were measured in cell, supernatant or lung tissue. 
Results: Indoleamine 2,3-dioxygenase (IDO) mRNA expression was elevated in HUC-MSCs primed with 5 ng/mL IFN-γ (P<0.001), and IFN-γ-exos and CON-exos were successfully extracted. LPS-induced inflammation resulted in decreased cell viability in A549 cells, and increased IL-1β, IL-6, TNF-α and ROS levels and NF-κB p65 and NLRP3 expression in A549 cells and mice(P<0.05 to P<0.001). Treatment with IFN-γ-exos and CON-exos increased cell viability and decreased the concentrations of IL-1β, and ROS, expression of NF-κB p65 and NLRP3, and the lung injury score, and these effects were more obvious for IFN-γ-exos(P<0.05 to P<0.001). 
Conclusion: IFN-γ-exos reduced oxidative stress and inflammatory responses in LPS-induced A549 cells and mice. The result demonstrated the therapeutic potential of IFN-γ-exos in LPS-induced ALI.
کلیدواژه‌ها
Acute lung injury؛ Exosomes؛ Mesenchymal stem cells؛ Interferon gamma؛ Inflammation
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