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Uluışık, Deniz, Keskin, Ercan, Özaydın, Tuğba, Öznurlu, Yasemin. (1402). Ameliorative effects of the melatonin on some cytokine levels, NF-κB immunoreactivity, and apoptosis in rats with cerulein-induced acute pancreatitis. , 27(3), 279-285. doi: 10.22038/ijbms.2023.69019.15045
Deniz Uluışık; Ercan Keskin; Tuğba Özaydın; Yasemin Öznurlu. "Ameliorative effects of the melatonin on some cytokine levels, NF-κB immunoreactivity, and apoptosis in rats with cerulein-induced acute pancreatitis". , 27, 3, 1402, 279-285. doi: 10.22038/ijbms.2023.69019.15045
Uluışık, Deniz, Keskin, Ercan, Özaydın, Tuğba, Öznurlu, Yasemin. (1402). 'Ameliorative effects of the melatonin on some cytokine levels, NF-κB immunoreactivity, and apoptosis in rats with cerulein-induced acute pancreatitis', , 27(3), pp. 279-285. doi: 10.22038/ijbms.2023.69019.15045
Uluışık, Deniz, Keskin, Ercan, Özaydın, Tuğba, Öznurlu, Yasemin. Ameliorative effects of the melatonin on some cytokine levels, NF-κB immunoreactivity, and apoptosis in rats with cerulein-induced acute pancreatitis. , 1402; 27(3): 279-285. doi: 10.22038/ijbms.2023.69019.15045

Ameliorative effects of the melatonin on some cytokine levels, NF-κB immunoreactivity, and apoptosis in rats with cerulein-induced acute pancreatitis

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 27، شماره 3، خرداد 2024، صفحه 279-285    اصل مقاله (967.67 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.69019.15045
نویسندگان
Deniz Uluışık* 1؛ Ercan Keskin1؛ Tuğba Özaydın2؛ Yasemin Öznurlu2
1University of Selçuk, Faculty of Veterinary Medicine, Department of Physiology, Turkey
2University of Selçuk, Faculty of Veterinary Medicine, Department of Histology and Embryology, Turkey
چکیده
Objective(s): Investigating the ameliorative effects of melatonin on cytokine levels, apoptosis, and NF-κB immunoreactivity in rats with cerulein-induced acute pancreatitis.
Materials and Methods: Thirthy-two Wistar Albino rats were divided into four groups: Control group which didn’t undergo acute pancreatitis induction and was left without treatment, pancreatitis group in which the acute pancreatitis was induced by 2 successive intraperitoneal doses of cerulein at a 2-hour interval (50 µg/kg and then 25 µg/kg), melatonin-treated pancreatitis group which was intraperitoneally administrated with 50 mg/kg of melatonin, 30 min before each cerulein injection, and melatonin group which was intraperitoneally administrated with 2 successive doses of melatonin (50 mg/kg each) at a 2-hour interval. Pancreatic tissue and blood samples were taken from animals of all groups. IL-1β, TNF-α, and IL-10 levels were determined in blood samples. Apoptosis was determined by the TUNEL assay and the NF-κB was detected immunohistochemically in acinar cells of the exocrine pancreatic portion.
Results: IL-1β, TNF-α, and IL-10 levels in the acute pancreatitis group were significantly increased when compared to the control negative group. IL-1β and TNF-α levels in the melatonin-treated pancreatitis group were significantly lower than those of the acute pancreatitis group. While number of apoptotic cells and percentage of NF-κB immunopositive cells in the acute pancreatitis group were significantly increased compared to other groups and it was observed that these parameters were significantly reduced in the melatonin-treated pancreatitis group compared to the acute pancreatitis group.
Conclusion: These findings suggest that melatonin administration can significantly reduce the severity of acute pancreatitis in rats.
کلیدواژه‌ها
Acute pancreatitis؛ Apoptosis؛ Cytokine؛ Melatonin؛ NF-κB
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