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Mousavi, Mahboube, Mousavi, Aliasghar, Jamei, Behnam, Sameni, Hamidreza, Zarbakhsh, Sam, Aboutaleb Kadkhodaeian, Hamid. (1402). Classification, location, and intensity of granules in retinal pigment epithelium following sodium iodate injection in rat animal model. , 27(3), 286-296. doi: 10.22038/ijbms.2023.71194.15465
Mahboube Mousavi; Aliasghar Mousavi; Behnam Jamei; Hamidreza Sameni; Sam Zarbakhsh; Hamid Aboutaleb Kadkhodaeian. "Classification, location, and intensity of granules in retinal pigment epithelium following sodium iodate injection in rat animal model". , 27, 3, 1402, 286-296. doi: 10.22038/ijbms.2023.71194.15465
Mousavi, Mahboube, Mousavi, Aliasghar, Jamei, Behnam, Sameni, Hamidreza, Zarbakhsh, Sam, Aboutaleb Kadkhodaeian, Hamid. (1402). 'Classification, location, and intensity of granules in retinal pigment epithelium following sodium iodate injection in rat animal model', , 27(3), pp. 286-296. doi: 10.22038/ijbms.2023.71194.15465
Mousavi, Mahboube, Mousavi, Aliasghar, Jamei, Behnam, Sameni, Hamidreza, Zarbakhsh, Sam, Aboutaleb Kadkhodaeian, Hamid. Classification, location, and intensity of granules in retinal pigment epithelium following sodium iodate injection in rat animal model. , 1402; 27(3): 286-296. doi: 10.22038/ijbms.2023.71194.15465

Classification, location, and intensity of granules in retinal pigment epithelium following sodium iodate injection in rat animal model

Iranian Journal of Basic Medical Sciences
مقاله 4، دوره 27، شماره 3، خرداد 2024، صفحه 286-296    اصل مقاله (1.43 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71194.15465
نویسندگان
Mahboube Mousavi1، 2؛ Aliasghar Mousavi3؛ Behnam Jamei4؛ Hamidreza Sameni1، 2؛ Sam Zarbakhsh1، 2؛ Hamid Aboutaleb Kadkhodaeian* 1، 2، 3
1Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran
2Department of Anatomy, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran
3Visual Health Center, Semnan University of Medical Sciences, Semnan, Iran
4Neurosciences Research Center, Iran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Age-related macular degeneration (AMD) is one of the eye diseases that can affect a person’s central vision. Retinal pigment epithelium (RPE) cells are damaged in this medical condition and some pigments are presented in these cells. Here, we aimed to investigate melanin and lipofuscin granules of RPE cells as a precursor of AMD. 
Materials and Methods: Hooded rats (n=18) were divided into two groups and received 100 μl of sodium iodate (SI) into the retro-orbital sinus of their eyes at 40 and 60 mg/kg doses. The total number of melanin and lipofuscin granules, different types of granules, cytoplasmic dispersion of granules as well as morphological changes in the shape and number of nuclei of RPE cells were evaluated over the course of 1-30 days.
Results: The total number of melanin pigments increases over time at a dose of 40 mg/kg and decreases at a dose of 60 mg/kg. Also, the total number of lipofuscin granules in 40 mg/kg increases over time and decreases in 60 mg/kg. Autofluorescent intensity (AF) is also increased at 40 mg/kg, but at 60 mg/kg, the highest intensity is on day 7. Also, the highest number of multinucleated giant cells was on day 7 at 60 mg/kg and the most changes in cell appearance due to sodium iodate injection were seen on the first day after injection. 
Conclusion: We demonstrated that granules and autofluorescent intensity appear to decrease at high doses of sodium iodate, which is similar to the advanced stage of the AMD disease, where the number of granules and AF intensity increase in the middle and even early stages of the disease.
کلیدواژه‌ها
Age-related macular - degeneration؛ Fluorescent light intensity؛ Lipofuscin؛ Melanin؛ Sodium iodate
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