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Khan, Aamir, Sharma, Sumit, Das, Anwesha, Alam, Mumtaz, Syed, Mansoor Ali, Haque, Syed. (1402). D-Pinitol improves cognitive dysfunction and neuronal damage induced by isoproterenol via modulation of NF-κB/BDNF/GFAP signaling in Swiss albino mice. , 27(3), 326-334. doi: 10.22038/ijbms.2023.72207.15698
Aamir Khan; Sumit Sharma; Anwesha Das; Mumtaz Alam; Mansoor Ali Syed; Syed Ehtaishamul Haque. "D-Pinitol improves cognitive dysfunction and neuronal damage induced by isoproterenol via modulation of NF-κB/BDNF/GFAP signaling in Swiss albino mice". , 27, 3, 1402, 326-334. doi: 10.22038/ijbms.2023.72207.15698
Khan, Aamir, Sharma, Sumit, Das, Anwesha, Alam, Mumtaz, Syed, Mansoor Ali, Haque, Syed. (1402). 'D-Pinitol improves cognitive dysfunction and neuronal damage induced by isoproterenol via modulation of NF-κB/BDNF/GFAP signaling in Swiss albino mice', , 27(3), pp. 326-334. doi: 10.22038/ijbms.2023.72207.15698
Khan, Aamir, Sharma, Sumit, Das, Anwesha, Alam, Mumtaz, Syed, Mansoor Ali, Haque, Syed. D-Pinitol improves cognitive dysfunction and neuronal damage induced by isoproterenol via modulation of NF-κB/BDNF/GFAP signaling in Swiss albino mice. , 1402; 27(3): 326-334. doi: 10.22038/ijbms.2023.72207.15698

D-Pinitol improves cognitive dysfunction and neuronal damage induced by isoproterenol via modulation of NF-κB/BDNF/GFAP signaling in Swiss albino mice

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 27، شماره 3، خرداد 2024، صفحه 326-334    اصل مقاله (1.11 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.72207.15698
نویسندگان
Aamir Khan1؛ Sumit Sharma1؛ Anwesha Das2؛ Mumtaz Alam2؛ Mansoor Ali Syed3؛ Syed Ehtaishamul Haque* 1
1Department of Pharmacology, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi–110062, India
2Drug Design and Medicinal Chemistry Lab., Department of Pharmaceutical Chemistry, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, New Delhi–110062, India
3Department of Biotechnology, Jamia Millia Islamia, New Delhi 110025, India
چکیده
Objective(s): Neurological disorders are the world’s most distressing problem. The adverse effects of current medications continue to compel scientists to seek safer, more effective, and economically affordable alternatives. In this vein, we explored the effect of D-Pinitol on isoproterenol-induced neurotoxicity in mice.
Materials and Methods: Forty-two mice were randomly distributed into 7 groups each having 6 animals. Group I; received saline. Group II; received isoproterenol (ISO) 15 mg/kg/day, s.c. for 20 days. Group III, IV; received 50 and 100 mg/kg/day/oral of D-Pinitol, respectively along with ISO for 20 days. Group V; received D-Pinitol 100 mg/kg/day/oral for 20 days. Group VI; received propranolol 20 mg/kg/day/oral and ISO for 20 days. Group VII; received propranolol 20 mg/kg/day/oral for 20 days. On the 21st day after behavioral tests, blood was collected and mice were sacrificed for various biochemical, histopathological, and immunohistochemical analyses.
Results: Chronic administration of isoproterenol caused neurotoxicity, cognitive dysfunction, and histopathological changes in the brain as evidenced by increase in GFAP, oxidative stress (via SOD, CAT, TBARS, and GSH), neuroinflammation  (NF-kB, TNF-α, IL-6, and IL-10), and decrease in AchE and BDNF. Co-administration of D-Pinitol (100 mg/kg) significantly prevented these pathological alterations. The cognitive improvement was also observed through the forced swim test, elevated plus maze test, and rotarod test.
Conclusion: Our findings on D-Pinitol thus clearly established its neuroprotective role in ISO-induced neurodegeneration in Swiss albino mice.
کلیدواژه‌ها
D-Pinitol؛ Inflammation؛ Isoproterenol؛ Neuroprotection؛ Neurotoxicity؛ Oxidative stress
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