Role of cell surface proteins and toll-like receptors in the pathogenesis of Streptococcus pneumoniae | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 11، دوره 27، شماره 2، اردیبهشت 2024، صفحه 214-222 اصل مقاله (678.38 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.72584.15791 | ||
| نویسندگان | ||
| Nurul Fathiyah Zaipul Anuar1؛ Mohd Nasir Mohd Desa2؛ Jamal Hussaini3، 4؛ Eng Hwa Wong5؛ Vanitha Mariappan6؛ Kumutha Malar Vellasamy7؛ Navindra Kumari Palanisamy* 1، 3 | ||
| 1Institute of Medical Molecular Biotechnology (IMMB), Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia | ||
| 2Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, (UPM), Serdang, elangor, Malaysia | ||
| 3Department of Medical Microbiology & Parasitology, Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Selangor, Malaysia | ||
| 4Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, Sungai Buloh, Selangor, Malaysia | ||
| 5School of Medicine, Taylor’s University Lakeside Campus, Subang Jaya, Selangor, Malaysia | ||
| 6Center for Toxicology and Health Risk Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia | ||
| 7Department of Medical Microbiology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia | ||
| چکیده | ||
| Objective(s): Pneumococcal cell wall (PCW) is an inflammatory component in Streptococcus pneumoniae. The cell surface proteins and the toll-like receptors (TLR) signaling response were investigated in the human lung epithelial (A549) cells inoculated with PCW of different serotypes. Materials and Methods: The presence of genes encoding these proteins was determined using polymerase chain reaction (PCR). The structure of the cell walls was analyzed by proton nuclear magnetic resonance (1H NMR). The A549 cell line was challenged with PCW extracts of different serotypes. RNA from the infected host cells was extracted and tested against a total of 84 genes associated with TLR signaling pathways (TLR 1-6 and 10) using RT2 Profiler PCR Array. Results: Cell surface proteins; ply, lytA, nanA, nanB, and cbpD genes were present in all serotypes. The distribution and structure of surface protein genes suggest behavioral changes in the molecules, contributing to the resilience of the strains to antibiotic treatment. Conclusion: TLR2 showed the highest expression, while serotypes 1, 3, and 5 induced higher TNFα and IL-1α, suggesting to be more immunogenic than the other strains tested. | ||
| کلیدواژهها | ||
| A549 cell line؛ Cell wall؛ Serotype؛ Streptococcus pneumoniae؛ Surface proteins؛ Toll-like receptors | ||
| مراجع | ||
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