سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Hashemi, Seyedeh Sara, Mohammadi, Ali-Akbar, Kian, Mehdi, Rafati, Alireza, Ghaedi, Mojtaba, Ghafari, Behzad. (1402). Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury. , 27(2), 223-232. doi: 10.22038/ijbms.2023.69930.15210
Seyedeh Sara Hashemi; Ali-Akbar Mohammadi; Mehdi Kian; Alireza Rafati; Mojtaba Ghaedi; Behzad Ghafari. "Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury". , 27, 2, 1402, 223-232. doi: 10.22038/ijbms.2023.69930.15210
Hashemi, Seyedeh Sara, Mohammadi, Ali-Akbar, Kian, Mehdi, Rafati, Alireza, Ghaedi, Mojtaba, Ghafari, Behzad. (1402). 'Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury', , 27(2), pp. 223-232. doi: 10.22038/ijbms.2023.69930.15210
Hashemi, Seyedeh Sara, Mohammadi, Ali-Akbar, Kian, Mehdi, Rafati, Alireza, Ghaedi, Mojtaba, Ghafari, Behzad. Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury. , 1402; 27(2): 223-232. doi: 10.22038/ijbms.2023.69930.15210

Fabrication and evaluation of the regenerative effect of a polycaprolactone/chitosan nanofibrous scaffold containing bentonite nanoparticles in a rat model of deep second-degree burn injury

Iranian Journal of Basic Medical Sciences
مقاله 12، دوره 27، شماره 2، اردیبهشت 2024، صفحه 223-232    اصل مقاله (1.03 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.69930.15210
نویسندگان
Seyedeh Sara Hashemi1؛ Ali-Akbar Mohammadi1، 2؛ Mehdi Kian3، 4؛ Alireza Rafati5؛ Mojtaba Ghaedi* 6؛ Behzad Ghafari2
1Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
2Division of Plastic and Reconstructive Surgery, Department of Surgery, Shiraz University of Medical Sciences, Shiraz, Fars, Iran
3Department of Comparative Biomedical Sciences, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Fars, Iran
4Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Fars, Iran
5Division of Pharmacology and Pharmaceutical Chemistry, Sarvestan Branch, Islamic Azad University, Sarvestan, Fars, Iran
6Department of Surgery, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Fars, Iran
چکیده
Objective(s): In the present study, we evaluated the effect of a nanofibrous scaffold including polycaprolactone (PCL), chitosan (CHT), and bentonite nanoparticles (Ben-NPS) on wound healing in order to introduce a novel dressing for burn wounds. 
Materials and Methods: PCL, PCL/CHT, and PCL/CHT/Ben-NPS nanofibrous scaffolds were fabricated by the electrospinning technique. Their structural and physiochemical characteristics were investigated by Fourier-transform infrared spectroscopy (FTIR) analysis, scanning electron microscopy (SEM), tensile strength, water contact angle, as well as, swelling and degradation profiles test. The disc diffusion assay was carried out to investigate the antibacterial potential of the scaffolds. In addition, the cell viability and proliferation ability of human dermal fibroblasts (HDFs) on the scaffolds were assessed using MTT assay as well as SEM imaging. The wound-healing property of the nanofibrous scaffolds was evaluated by histopathological investigations during 3,7, and 14 days in a rat model of burn wounds. 
Results: SEM showed that all scaffolds had three-dimensional, beadles-integrated structures. Adding Ben-NPS into the PCL/CHT polymeric composite significantly enhanced the mechanical, swelling, and antibacterial properties. HDFs had the most cell viability and proliferation values on the PCL/CHT/Ben-NPS scaffold. Histopathological evaluation in the rat model revealed that dressing animal wounds with the PCL/CHT/Ben-NPS scaffold promotes wound healing. 
Conclusion: The PCL/CHT/Ben-NPS scaffold has promising regenerative properties for accelerating skin wound healing.
کلیدواژه‌ها
Bentonite-nanoparticles؛ Nanofibrous scaffold؛ Polycaprolactone؛ Rat؛ Wound healing
مراجع
1. Shpichka A, Butnaru D, Bezrukov EA, Sukhanov RB, Atala A, Burdukovskii V, et al. Skin tissue regeneration for burn injury. Stem Cell Res Ther 2019; 10:94-120.
2. Hashemi SS, Mohammadi AA, Kabiri H, Hashempoor MR, Mahmoodi M, Amini M, et al. The healing effect of Wharton’s jelly stem cells seeded on biological scaffold in chronic skin ulcers: A randomized clinical trial. J Cosmet Dermatol 2019; 18:1961-1967.
3. Kwan PO, Tredget EE. Biological principles of scar and contracture. Hand Clin 2017; 33:277-292.
4. Hashemi SS, Mohammadi AA, Moshirabadi K, Zardosht M. Effect of dermal fibroblasts and mesenchymal stem cells seeded on an amniotic membrane scaffold in skin regeneration: A case series. J Cosmet Dermatol 2021; 20:4040-4047.
5. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: A cellular perspective. Physiol Rev 2019; 99:665-706.
6. Negut I, Dorcioman G, Grumezescu V. Scaffolds for Wound H ealing Applications. Polymers (Basel) 2020; 12:2010-2029.
7. Amirsadeghi A, Jafari A, Eggermont LJ, Hashemi SS, Bencherif SA, Khorram M. Vascularization strategies for skin tissue engineering. Biomater Sci 2020; 8:4073-4094.
8. Mohamed RM, Yusoh K. A review on the recent research of polycaprolactone (PCL). Adv Mat Res 2016; 1134:249-255.
9. Hashemi SS, Rajabi SS, Mahmoudi R, Ghanbari A, Zibara K, Barmak MJ. Polyurethane/chitosan/hyaluronic acid scaffolds: providing an optimum environment for fibroblast growth. J Wound Care 2020; 29:586-596.
10. Devi N, Dutta J. Preparation and characterization of chitosan-bentonite nanocomposite films for wound healing application. Int J Biol Macromol 2017; 104:1897-1904.
11. Khan ZA, Jamil S, Akhtar A, Bashir MM, Yar M. Chitosan based hybrid materials used for wound healing applications-A short review. Int J Polym Mater 2019; 69:419-436.
12. Emami-Razavi S, Esmaeili N, Forouzannia S, Amanpour S, Rabbani S, Mohagheghi AAM. Effect of bentonite on skin wound healing: experimental study in the rat model. Acta Med Iran 2006:235-240.
13. Phull QZ, Arain AA, Ansari MA, Memon AR. Wound Healing Effects of Bentonite: A Rabbit Model Experimental Study. Biomed J 2018; 1:7683-7686.
14. Nabgui A, El Assimi T, El Meziane A, Luinstra GA, Raihane M, Gouhier G, et al. Synthesis and antibacterial behavior of Bio-composite materials-based on Poly (ε-caprolactone)/Bentonite. Eur Polym J 2021:110602.
15. Chakraborty S, Pimentel CJM. Bentonite reinforced chitosan scaffold: effect of bentonite exfoliation on scaffold properties. Philipp J Sci 2020; 149:1115-1121.
16. Nozari M, Gholizadeh M, Zahiri Oghani F, Tahvildari K. Studies on novel chitosan/alginate and chitosan/bentonite flexible films incorporated with ZnO nano particles for accelerating dermal burn healing: In vivo and in vitro evaluation. Int J Biol Macromol 2021; 184:235-249.
17. Islam MS, Ang BC, Andriyana A, Afifi AM. A review on fabrication of nanofibers via electrospinning and their applications. SN Applied Sciences 2019; 1:1-16.
18. Mulholland EJ. Electrospun biomaterials in the treatment and prevention of scars in skin wound healing. Front Bioeng Biotechnol 2020; 8:481.
19. Amirsadeghi A, Khorram M, Hashemi SS. Preparation of multilayer electrospun nanofibrous scaffolds containing soluble eggshell membrane as potential dermal substitute. J Biomed Mater Res A 2021; 109:1812-1827.
20. Hashemi S-S, Mohammadi AA, Rajabi S-S, Sanati P, Rafati A, Kian M, et al. Preparation and evaluation of a polycaprolactone/chitosan/propolis fibrous nanocomposite scaffold as a tissue engineering skin substitute. BioImpacts 2023; 13:275-287.
21. Hashemi SS, Saadatjo Z, Mahmoudi R, Delaviz H, Bardania H, Rajabi SS, et al. Preparation and evaluation of polycaprolactone/chitosan/Jaft biocompatible nanofibers as a burn wound dressing. Burns 2022; 48:1690-1705.
22. Hotaling NA, Bharti K, Kriel H, Simon CG, Jr. DiameterJ: A validated open source nanofiber diameter measurement tool. Biomaterials 2015; 61:327-338.
23. Beck EC, Jarrell DK, Lyons AC, Vanderslice EJ, VeDepo MC, Jacot JG. Assessment of electrospun cardiac patches made with sacrificial particles and polyurethane‐polycaprolactone blends. J Biomed Mater Res A 2021; 109(11):2154-2163.
24. Oliveira S, Felizardo T, Amorim S, Mithieux SM, Pires RA, Reis RL, et al. Tubular fibrous scaffolds functionalized with tropoelastin as a small-diameter vascular graft. Biomacromolecules 2020; 21:3582-3595.
25. Sadeghi A, Moztarzadeh F, Aghazadeh Mohandesi J. Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering. Int J Biol Macromol 2019; 121:625-632.
26. Langwald SV, Ehrmann A, Sabantina L. Measuring physical properties of electrospun nanofiber mats for different biomedical applications. Membranes (Basel) 2023; 13:488.
27. Sharifi F, Atyabi SM, Norouzian D, Zandi M, Irani S, Bakhshi H. Polycaprolactone/carboxymethyl chitosan nanofibrous scaffolds for bone tissue engineering application. Int J Biol Macromol 2018; 115:243-248.
28. Zahedi E, Esmaeili A, Eslahi N, Shokrgozar MA, Simchi A. Fabrication and characterization of core-shell electrospun fibrous mats containing medicinal herbs for wound healing and skin tissue engineering. Mar Drugs 2019; 17:27.
29. Banik N, Jahan S, Mostofa S, Kabir H, Sharmin N, Rahman M, et al. Synthesis and characterization of organoclay modified with cetylpyridinium chloride. Bangladesh J Sci Ind Res 2015; 50:65-70.
30. Holtzer M, Bobrowski A, Grabowska B. Montmorillonite: a comparison of methods for its determination in foundry bentonites. Metalurgija 2011; 50:119-122.
31. Zaitan H, Bianchi D, Achak O, Chafik T. A comparative study of the adsorption and desorption of o-xylene onto bentonite clay and alumina. J Hazard Mater 2008; 153:852-859.
32. Bacakova L, Zikmundova M, Pajorova J, Broz A, Filova E, Blanquer A, et al. Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Synthetic Polymers. In: Margarita S, Roumen Z, editors. Applications of Nanobiotechnology. Rijeka: IntechOpen; 2020. p. Ch. 3.
33. Nosrati H, Aramideh Khouy R, Nosrati A, Khodaei M, Banitalebi-Dehkordi M, Ashrafi-Dehkordi K, et al. Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis. J Nanobiotechnology 2021; 19:1-21.
34. Gallagher A, Ní Annaidh A, Bruyère K. Dynamic tensile properties of human skin.  IRCOBI Conference 2012, 12-14 September 2012, Dublin (Ireland): IRCOBI 2012.
35. Barua S, Dutta N, Karmakar S, Chattopadhyay P, Aidew L, Buragohain AK, et al. Biocompatible high performance hyperbranched epoxy/clay nanocomposite as an implantable material. Biomed Mater 2014; 9:025006.
36. Huang Y, Dan N, Dan W, Zhao W. Reinforcement of polycaprolactone/chitosan with nanoclay and controlled release of curcumin for wound dressing. ACS Omega 2019; 4:22292-22301.
37. Zamrud Z, Ng W, Salleh H, editors. Effect of bentonite nanoclay filler on the properties of bioplastic based on sago starch. ICoBiomasSE; 2021: IOP Publishing.
38. Zhu X, Cui W, Li X, Jin Y. Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules 2008; 9:1795-1801.
39. Park J-H, Shin H-J, Kim MH, Kim J-S, Kang N, Lee J-Y, et al. Application of montmorillonite in bentonite as a pharmaceutical excipient in drug delivery systems. J Pharm Investig 2016; 46:363-375.
40. Widiyanti P, Gayatri DB, Rudyardjo DI. Synthesis and characterization scaffold chitosan/poly (ε-caprolactone) as candidate for skin tissue engineering in burns. Mal J Med Health Sci 2021; 17:24-29.
41. Neves SC, Teixeira LSM, Moroni L, Reis RL, Van Blitterswijk CA, Alves NM, et al. Chitosan/Poly (ɛ-caprolactone) blend scaffolds for cartilage repair. Biomaterials 2011; 32:1068-1079.
42. Kirbay FO, Yalcinkaya EE, Atik G, Evren G, Unal B, Demirkol DO, et al. Biofunctionalization of PAMAM-montmorillonite decorated poly (E-caprolactone)-chitosan electrospun nanofibers for cell adhesion and electrochemical cytosensing. Biosens Bioelectron 2018; 109:286-294.
43. Chang H-I, Wang Y. Cell Responses to Surface and Architecture of Tissue Engineering Scaffolds. In: Daniel E, editor. Regenerative Medicine and Tissue Engineering - Cells and Biomaterials. Rijeka: IntechOpen; 2011. p. Ch. 27.
44. Sakr MA, Mohamed MG, Wu R, Shin SR, Kim D, Kim K, et al. Development of bentonite-gelatin nanocomposite hybrid hydrogels for tissue engineering. Appl Clay Sci 2020; 199:105860.
45. Depan D, Kumar AP, Singh RP. Cell proliferation and controlled drug release studies of nanohybrids based on chitosan-g-lactic acid and montmorillonite. Acta Biomater 2009; 5:93-100.
46. Niemczyk-Soczynska B, Gradys A, Sajkiewicz P. Hydrophilic surface functionalization of electrospun nanofibrous scaffolds in tissue engineering. Polymers (Basel) 2020; 12:2636.
47. Maliszewska I, Czapka T. Electrospun polymer nanofibers with antimicrobial activity. Polymers (Basel) 2022; 14:1661-1692.
48. Williams LB, Metge DW, Eberl DD, Harvey RW, Turner AG, Prapaipong P, et al. What makes a natural clay antibacterial? Environ Sci Technol 2011; 45:3768-3773.
49. Sajjad W, Khan T, Ul-Islam M, Khan R, Hussain Z, Khalid A, et al. Development of modified montmorillonite-bacterial cellulose nanocomposites as a novel substitute for burn skin and tissue regeneration. Carbohydr Polym 2019; 206:548-556.
50. Ul-Islam M, Khan T, Khattak WA, Park JK. Bacterial cellulose-MMTs nanoreinforced composite films: novel wound dressing material with antibacterial properties. Cellulose 2013; 20:589-596.
51. Hu CH, Xu ZR, Xia MS. Antibacterial effect of Cu2+-exchanged montmorillonite on Aeromonas hydrophila and discussion on its mechanism. Vet Microbiol 2005; 109:83-88.
52. Dastjerdi R, Sharafi M, Kabiri K, Mivehi L, Samadikuchaksaraei A. An acid-free water-born quaternized chitosan/montmorillonite loaded into an innovative ultra-fine bead-free water-born nanocomposite nanofibrous scaffold; in vitro and in vivo approaches. Biomed Mater 2017; 12:045014.

آمار
تعداد مشاهده مقاله: 768
تعداد دریافت فایل اصل مقاله: 545