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Khosrojerdi, Somayeh, Gholami, Leila, Khazaei, Majid, Hashemzadeh, Alireza, Darroudi, Majid, Kazemi Oskuee, Reza. (1402). Synthesis and evaluation of gene delivery vectors based on PEI-modified metal-organic framework (MOF) nanoparticles. , 27(2), 203-213. doi: 10.22038/ijbms.2023.71892.15644
Somayeh Khosrojerdi; Leila Gholami; Majid Khazaei; Alireza Hashemzadeh; Majid Darroudi; Reza Kazemi Oskuee. "Synthesis and evaluation of gene delivery vectors based on PEI-modified metal-organic framework (MOF) nanoparticles". , 27, 2, 1402, 203-213. doi: 10.22038/ijbms.2023.71892.15644
Khosrojerdi, Somayeh, Gholami, Leila, Khazaei, Majid, Hashemzadeh, Alireza, Darroudi, Majid, Kazemi Oskuee, Reza. (1402). 'Synthesis and evaluation of gene delivery vectors based on PEI-modified metal-organic framework (MOF) nanoparticles', , 27(2), pp. 203-213. doi: 10.22038/ijbms.2023.71892.15644
Khosrojerdi, Somayeh, Gholami, Leila, Khazaei, Majid, Hashemzadeh, Alireza, Darroudi, Majid, Kazemi Oskuee, Reza. Synthesis and evaluation of gene delivery vectors based on PEI-modified metal-organic framework (MOF) nanoparticles. , 1402; 27(2): 203-213. doi: 10.22038/ijbms.2023.71892.15644

Synthesis and evaluation of gene delivery vectors based on PEI-modified metal-organic framework (MOF) nanoparticles

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 27، شماره 2، اردیبهشت 2024، صفحه 203-213    اصل مقاله (657.59 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71892.15644
نویسندگان
Somayeh Khosrojerdi1؛ Leila Gholami2؛ Majid Khazaei3؛ Alireza Hashemzadeh4؛ Majid Darroudi5؛ Reza Kazemi Oskuee* 6
1Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
3Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4Targeted Drug Delivery Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran
6Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Zirconium-based metal-organic frameworks (MOFs) nanostructures, due to their capability of easy surface modification, are considered interesting structures for delivery. In the present study, the surfaces of UIO-66 and NH2-UIO-66 MOFs were modified by polyethyleneimine (PEI) 10000 Da, and their efficiency for plasmid delivery was evaluated. 
Materials and Methods: Two different approaches, were employed to prepare surface-modified nanoparticles. The physicochemical characteristics of the resulting nanoparticles, as well as their transfection efficiency and cytotoxicity, were investigated on the A549 cell line. 
Results: The sizes of DNA/nanocarriers for PEI-modified UIO-66 (PEI-UIO-66) were between 212–291 nm and 267–321 nm for PEI 6-bromohexanoic acid linked UIO-66 (PEI-HEX-UIO-66). The zeta potential of all was positive with the ranges of +16 to +20 mV and +23 to +26 mV for PEI-UIO-66 and PEI-HEX-UIO-66, respectively. Cellular assay results showed that the PEI linking method had a higher rate of gene transfection efficiency with minimal cytotoxicity than the wet impregnation method. The difference between transfection of modified nanoparticles compared to the PEI 10 kDa was not significant but the PEI-HEX-UIO-66 showed less cytotoxicity. 
Conclusion: The present study suggested that the post-synthetic modification of MOFs with PEI 10000 Da through EDC/NHS+6-bromohexanoic acid reaction can be considered as an effective approach for modifying MOFs’ structure in order to obtain nanoparticles with better biological function in the gene delivery process.
کلیدواژه‌ها
Gene delivery؛ Metal-organic frameworks؛ Polyethyleneimine؛ Transfection؛ UIO-66
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