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Eisvand, Farhad, Rezvani, Kasra, Hosseinzadeh, Hossein, Razavi, Bibi Marjan. (1402). Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression. , 27(1), 90-96. doi: 10.22038/ijbms.2023.71019.15425
Farhad Eisvand; Kasra Rezvani; Hossein Hosseinzadeh; Bibi Marjan Razavi. "Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression". , 27, 1, 1402, 90-96. doi: 10.22038/ijbms.2023.71019.15425
Eisvand, Farhad, Rezvani, Kasra, Hosseinzadeh, Hossein, Razavi, Bibi Marjan. (1402). 'Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression', , 27(1), pp. 90-96. doi: 10.22038/ijbms.2023.71019.15425
Eisvand, Farhad, Rezvani, Kasra, Hosseinzadeh, Hossein, Razavi, Bibi Marjan. Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression. , 1402; 27(1): 90-96. doi: 10.22038/ijbms.2023.71019.15425

Alpha-mangostin decreases high glucose-induced damage on human umbilical vein endothelial cells by increasing autophagic protein expression

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 27، شماره 1، فروردین 2024، صفحه 90-96    اصل مقاله (729.82 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71019.15425
نویسندگان
Farhad Eisvand1؛ Kasra Rezvani1؛ Hossein Hosseinzadeh1، 2؛ Bibi Marjan Razavi* 1، 3
1Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
3Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Diabetes is a chronic disorder that occurs as a result of impaired glucose metabolism. In hyperglycaemic states, the balance between oxidative stress and antioxidant enzymes is disrupted leading to oxidative damage and cell death. In addition,  impaired autophagy leads to the storage of dysfunctional proteins and cellular organelles in the cell. Hence, the cytoprotective function of autophagy may be disrupted by high glucose conditions. Alpha-mangostin (A-MG) is an essential xanthone purified from the mangosteen fruit. The different pharmacological benefits of alpha-mangostin, including antioxidant, anti-obesity, and antidiabetic, were demonstrated. 
Materials and Methods: We evaluated the protective influence of A-MG on autophagic response impaired by high concentrations of glucose in human umbilical vein endothelial cells (HUVECs). The HUVECs were treated with various glucose concentrations (5-60 mM) and A-MG (1.25-10 μM) for three days. Then, HUVECs were treated with 60 mM of glucose+2.5 μM of A-MG to measure viability, ROS, and NO content. Finally, the levels of autophagic proteins including LC3, SIRT1, and beclin 1 were evaluated by western blot.
Results: The results expressed that high glucose condition (60 mM) decreased viability and increased ROS and NO content in HUVECs. In addition, LC3, SIRT1, and beclin 1 protein levels declined when HUVECs were exposed to the high concentration of glucose. A-MG reversed these detrimental effects and elevated autophagic protein levels.
Conclusion: Our data represent that A-MG protects HUVECs against high glucose conditions by decreasing ROS and NO generation as well as increasing the expression of autophagy proteins.
کلیدواژه‌ها
Alpha-mangostin؛ Autophagy؛ Beclin 1؛ Diabetes؛ Garcinia mangostana؛ HUVEC؛ LC3؛ SIRT1
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