Wound healing effects of dexpanthenol-loaded core/shell electrospun nanofibers: Implication of oxidative stress in wound healing | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 14، دوره 27، شماره 1، فروردین 2024، صفحه 97-106 اصل مقاله (663.99 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71412.15526 | ||
| نویسندگان | ||
| Seyede Sahar Hashemi1؛ Mahmoud Najari1؛ Milad Parvin2؛ Mohammad Mehdi Kalani3؛ Majid Assadi4؛ Ramin Seyedian5؛ Sasan Zaeri* 5 | ||
| 1Student Research Committee, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| 2Department of Oral & Maxillofacial Surgery, School of Dentistry, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| 3Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran | ||
| 4Nuclear Medicine and Molecular Imaging Research Center, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| 5Department of Pharmacology, School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran | ||
| چکیده | ||
| Objective(s): Knowing the detrimental role of oxidative stress in wound healing and the anti-oxidant properties of Dexpanthenol (Dex), we aimed to produce Dex-loaded electrospun core/shell nanofibers for wound healing study. The novelty was measuring oxidative stress in wounds to know how oxidative stress was affected by Dex-loaded fibers. Materials and Methods: TPVA solution containing Dex 6% (w/v) (core) and PVA/chitosan solution (shell) were coaxially electrospun with variable injection rates of the shell solution. Fibers were then tested for physicochemical properties, drug release profile, and effects on wound healing. Levels of tissue lipid peroxidation and superoxide dismutase activity were measured. Results: Fibers produced at shell injection rate of 0.3 ml/hr (F3 fibers) showed core/shell structure with an average diameter of 252 nm, high hydrophilicity (swelling: 157% at equilibrium), and low weight loss (13.6%). Dex release from F3 fibers seemed to be ruled by the Fickian mechanism based on the Korsmeyer-Peppas model (R2 = 0.94, n = 0.37). Dex-loaded F3 fibers promoted fibroblast viability (128.4%) significantly on day 5 and also accelerated wound healing compared to the neat F3 fibers at macroscopic and microscopic levels on day 14 post-wounding. The important finding was a significant decrease in malondialdehyde (0.39 nmol/ mg protein) level and an increase in superoxide dismutase (5.29 unit/mg protein) activity in Dex-loaded F3 fiber-treated wound tissues. Conclusion: Dex-loaded core/shell fibers provided nano-scale scaffolds with sustained release profile that significantly lowered tissue oxidative stress. This finding pointed to the importance of lowering oxidative stress to achieve proper wound healing. | ||
| کلیدواژهها | ||
| Core/shell nanofiber؛ Dexpanthenol؛ Fibroblast؛ Mouse؛ Oxidative stress؛ Wound healing | ||
| مراجع | ||
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