سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Gao, Haiqi, Zhou, Yifan, Yu, Chundong, Wang, Guifa, Song, Wenwei, Zhang, Zixu, Lu, Lu, Xue, Meilan, Liang, Hui. (1402). Fucoidan alleviated autoimmune diabetes in NOD mice by regulating pancreatic autophagy through the AMPK/mTOR1/TFEB pathway. , 27(1), 31-38. doi: 10.22038/ijbms.2023.68739.14981
Haiqi Gao; Yifan Zhou; Chundong Yu; Guifa Wang; Wenwei Song; Zixu Zhang; Lu Lu; Meilan Xue; Hui Liang. "Fucoidan alleviated autoimmune diabetes in NOD mice by regulating pancreatic autophagy through the AMPK/mTOR1/TFEB pathway". , 27, 1, 1402, 31-38. doi: 10.22038/ijbms.2023.68739.14981
Gao, Haiqi, Zhou, Yifan, Yu, Chundong, Wang, Guifa, Song, Wenwei, Zhang, Zixu, Lu, Lu, Xue, Meilan, Liang, Hui. (1402). 'Fucoidan alleviated autoimmune diabetes in NOD mice by regulating pancreatic autophagy through the AMPK/mTOR1/TFEB pathway', , 27(1), pp. 31-38. doi: 10.22038/ijbms.2023.68739.14981
Gao, Haiqi, Zhou, Yifan, Yu, Chundong, Wang, Guifa, Song, Wenwei, Zhang, Zixu, Lu, Lu, Xue, Meilan, Liang, Hui. Fucoidan alleviated autoimmune diabetes in NOD mice by regulating pancreatic autophagy through the AMPK/mTOR1/TFEB pathway. , 1402; 27(1): 31-38. doi: 10.22038/ijbms.2023.68739.14981

Fucoidan alleviated autoimmune diabetes in NOD mice by regulating pancreatic autophagy through the AMPK/mTOR1/TFEB pathway

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 27، شماره 1، فروردین 2024، صفحه 31-38    اصل مقاله (431.1 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68739.14981
نویسندگان
Haiqi Gao1؛ Yifan Zhou2؛ Chundong Yu3؛ Guifa Wang1؛ Wenwei Song1؛ Zixu Zhang1؛ Lu Lu1؛ Meilan Xue* 1؛ Hui Liang* 4
1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Qingdao University, 308 Ningxia Road, Qingdao 266071, PR China
2Qingdao No.17 Middle School, 80 Hangzhou Road, Qingdao 266031, Shandong Province, PR China
3Department of Laboratory, Women and Children’s Hospital of Qingdao, Qingdao, Shandong 266034, PR China
4Department of Human Nutrition, College of Public Health, Qingdao University, Qingdao 266071, PR China
چکیده
Objective(s): The present study investigated the effect and its underlying mechanisms of fucoidan on Type 1 diabetes mellitus (T1DM) in non-obese diabetic (NOD) mice. 
Materials and Methods: Twenty 7-week-old NOD mice were used in this study, and randomly divided into two groups (10 mice in each group): the control group and the fucoidan treatment group (600 mg/kg. body weight). The weight gain, glucose tolerance, and fasting blood glucose level in NOD mice were detected to assess the development of diabetes. The intervention lasted for 5 weeks. The proportions of Th1/Th2 cells from spleen tissues were tested to determine the anti-inflammatory effect of fucoidan. Western blot was performed to investigate the expression levels of apoptotic markers and autophagic markers. Apoptotic cell staining was visualized through TdT-mediated dUTP nick-end labeling (TUNEL).
Results: The results suggested that fucoidan ameliorated T1DM, as evidenced by increased body weight and improved glycemic control of NOD mice. Fucoidan down-regulated the Th1/Th2 cells ratio and decreased Th1 type pro-inflammatory cytokines’ level. Fucoidan enhanced the mitochondrial autophagy level of pancreatic cells and increased the expressions of Beclin-1 and LC3B II/LC3B I. The expression of p-AMPK was up-regulated and p-mTOR1 was inhibited, which promoted the nucleation of transcription factor EB (TFEB), leading to autophagy. Moreover, fucoidan induced apoptosis of pancreatic tissue cells. The levels of cleaved caspase-9, cleaved caspase-3, and Bax were up-regulated after fucoidan treatment. 
Conclusion: Fucoidan could maintain pancreatic homeostasis and restore immune disorder through enhancing autophagy via the AMPK/mTOR1/TFEB pathway in pancreatic cells.
کلیدواژه‌ها
Apoptosis؛ Autophagy؛ Fucoidan؛ NOD mice؛ Type 1 diabetes
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