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Abadi, Banafshe, Abdesheikhi, Jahangir, Sedghy, Farnaz, Mahmoodi, Merat, Fallah, Hossein. (1402). Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line. , 14(2), 166-176. doi: 10.22038/ajp.2023.22935
Banafshe Abadi; Jahangir Abdesheikhi; Farnaz Sedghy; Merat Mahmoodi; Hossein Fallah. "Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line". , 14, 2, 1402, 166-176. doi: 10.22038/ajp.2023.22935
Abadi, Banafshe, Abdesheikhi, Jahangir, Sedghy, Farnaz, Mahmoodi, Merat, Fallah, Hossein. (1402). 'Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line', , 14(2), pp. 166-176. doi: 10.22038/ajp.2023.22935
Abadi, Banafshe, Abdesheikhi, Jahangir, Sedghy, Farnaz, Mahmoodi, Merat, Fallah, Hossein. Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line. , 1402; 14(2): 166-176. doi: 10.22038/ajp.2023.22935

Silibinin improved the function of T cells in peripheral blood mononuclear cells (PBMCs) co-cultured with U-87 MG cell line

Avicenna Journal of Phytomedicine
دوره 14، شماره 2، خرداد و تیر 2024، صفحه 166-176    اصل مقاله (1022.54 K)
نوع مقاله: Short communication
شناسه دیجیتال (DOI): 10.22038/ajp.2023.22935
نویسندگان
Banafshe Abadi1؛ Jahangir Abdesheikhi2؛ Farnaz Sedghy* 2؛ Merat Mahmoodi2؛ Hossein Fallah3
1Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran. Brain Cancer Research Core, Universal Scientific Education and Research Network (USERN), Kerman, Iran
2Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
3Department of Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
چکیده
Objective: Silibinin has exhibited antitumor activities. However, there are few reports about the immunomodulatory properties of silibinin on T lymphocyte function in the tumor microenvironment. Here, we determined the effects of silibinin on T cells of peripheral blood mononuclear cells (PBMCs), cultivated alone or with a human cell line of glioblastoma (U-87 MG).
Materials and Methods: The proliferation of T lymphocytes was assessed by MTT test in the presence of silibinin (15 and 45 µM). Also, total antioxidant capacity (TAC), the activity of superoxide dismutase-3 (SOD3), and the levels of two cytokines interferon gamma (IFN-γ) and tumor growth beta (TGF-β) were compared between treated and untreated PBMCs alone or co-cultured with U-87 cells.
Results: According to our results, silibinin raised the TAC levels and SOD3 activity in the PBMCs and in the co-culture condition. Moreover, silibinin-treated PBMCs showed higher IFN-γ levels and lower TGF-β levels. Interestingly, silibinin protected PBMCs against the U-87-induced suppression.
Conclusion: Altogether, these results proposed the immunomodulatory potential of silibinin on T cells of PBMCs, as well as its partially protective effects on PBMCs against the suppression induced by U-87 MG cells.
کلیدواژه‌ها
Silibinin؛ Glioblastoma؛ IFN-γ؛ TGF-β؛ Peripheral blood mononuclear cells (PBMCs)
آمار
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