MiR181-5p promotes pathogenic angiogenesis of hepatopulmonary syndrome by negatively regulating Wnt inhibitor WIF1 | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 11، دوره 26، شماره 12، اسفند 2023، صفحه 1460-1467 اصل مقاله (598.55 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.70689.15362 | ||
| نویسندگان | ||
| Dan Li1؛ Guihua Li1؛ Caiyi Li1؛ Congwen Yang* 2؛ Kaizhi Lu* 1 | ||
| 1Department of Anesthesiology, Southwest Hospital, Third Military Medical University, Chongqing, 400038, China | ||
| 2College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing, 400016, China | ||
| چکیده | ||
| Objective(s): Hepatopulmonary syndrome is a serious respiratory injury caused by chronic liver disease. Excessive pulmonary capillary angiogenesis is the key pathological event. However, the mechanism of microRNA regulatory pulmonary capillary angiogenesis is still unclear. Materials and Methods: The hepatopulmonary syndrome rat model was constructed by Common bile duct ligation (CBDL) surgery. The expression tread of miR181-5p and Wif1 was detected by qRT-PCR and western blot in various tissues and disease processes. Wif1 was predicted as one of the potential target genes of miR181-5p by bioinformatic assay. miR181-5p mimics and inhibitors were used to increase/decrease miR181-5p levels in pulmonary microvascular cells. And Wif-1 specific recombinant lentiviruses were used to up-regulate and down-regulate Wif1 in pulmonary microvascular cells. Then, CCK8, Transwell, and tube formation assay were used for pulmonary microvascular cell proliferation, migration, and tube formation. And Dual-luciferase reporter assay was used to assess that miR181-5p may direct regulate Wif-1 in HPS rats. Results: The result showed miR181-5p specifically activates the Wnt signaling pathway by inhibiting Wif1 and then promotes pulmonary microvascular cell proliferation, migration, and tube formation, thereby accelerating the process of HPS. We finally verified Wif1 as a novel and direct target of miR181-5p in HPS. Conclusion: Taken together, we revealed an important miR-181-5p/Wif1/Wnt pathway in regulating pathological angiogenesis. It will prove beneficial as a therapeutic strategy for hepatopulmonary syndrome. | ||
| کلیدواژهها | ||
| Angiogenesis؛ Hepatopulmonary - syndrome؛ MiR181-5p؛ Pulmonary microvascular - endothelial cells؛ Wnt inhibitory factor-1 | ||
| مراجع | ||
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