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Abdi, Hakimeh, Roshanravan, Marjan, Mirzavi, Farshad, Hosseinzadeh, Hossein, Mosaffa, Fatemeh. (1402). Crocin’s effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure. , 26(12), 1431-1437. doi: 10.22038/ijbms.2023.70859.15392
Hakimeh Abdi; Marjan Roshanravan; Farshad Mirzavi; Hossein Hosseinzadeh; Fatemeh Mosaffa. "Crocin’s effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure". , 26, 12, 1402, 1431-1437. doi: 10.22038/ijbms.2023.70859.15392
Abdi, Hakimeh, Roshanravan, Marjan, Mirzavi, Farshad, Hosseinzadeh, Hossein, Mosaffa, Fatemeh. (1402). 'Crocin’s effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure', , 26(12), pp. 1431-1437. doi: 10.22038/ijbms.2023.70859.15392
Abdi, Hakimeh, Roshanravan, Marjan, Mirzavi, Farshad, Hosseinzadeh, Hossein, Mosaffa, Fatemeh. Crocin’s effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure. , 1402; 26(12): 1431-1437. doi: 10.22038/ijbms.2023.70859.15392

Crocin’s effect on phenotype switching of J774A.1 macrophages depends on their polarization state during exposure

Iranian Journal of Basic Medical Sciences
مقاله 7، دوره 26، شماره 12، اسفند 2023، صفحه 1431-1437    اصل مقاله (677.82 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.70859.15392
نویسندگان
Hakimeh Abdi1؛ Marjan Roshanravan1؛ Farshad Mirzavi2؛ Hossein Hosseinzadeh3، 4؛ Fatemeh Mosaffa* 5
1Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
2Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran
3Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
5Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Macrophages exhibit versatile phenotypes, with M1 macrophages releasing inflammatory cytokines and possessing microbicidal activities, while M2 macrophages release anti-inflammatory cytokines and contribute to tissue repair. The M1/M2 imbalance plays a significant role in various pathological processes. Crocin, known for its antioxidant properties and ability to eliminate free radicals, has been investigated for its potential anti-inflammatory effects. We examined the effect of the primary activation state of macrophages on their phenotype switching when exposed to crocin.
Materials and Methods: The crocin impact on macrophage viability was evaluated by MTT. TNF-α, IL-6, and IL-10 secretion, as well as Nos2/Arg1 ratio, were measured in cells treated with crocin or LPS+IFN-γ (M1 inducers), in cells concurrently treated with crocin and LPS+IFN-γ or in cells pretreated with crocin before M1 induction.
Results: Crocin did not show any toxicity at the concentration of 500 µM or lower. When uncommitted macrophages were exposed to crocin (25-100 µM), it elevated certain M1 activity indicators, including Nos2/Arg1 ratio and TNF-α secretion, but not IL-6. Crocin in concurrent treatment with LPS+IFN-γ prevented the increase in M1 indicators, Nos2/Arg1 ratio, and TNF-α secretion. However, pretreatment of cells with crocin before the addition of LPS+IFN-γ did not reverse M1 induction in macrophages; instead, it further increased the Nos2/Arg1 ratio and TNF-α secretion. IL-10 was not detectable in any of the experimental groups.
Conclusion: It appears that the modulatory effects of crocin on macrophage M1/M2 phenotype switching partly depend on the presence or absence of inflammatory mediators and, accordingly, the initial state of macrophage commitment.
کلیدواژه‌ها
Arg1؛ Crocin؛ Inflammation؛ Macrophage؛ Nos2؛ Saffron
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