سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Cakmak, Fatma, Kucukler, Sefa, Gur, Cihan, Comakli, Selim, Ileriturk, Mustafa, Kandemir, Fatih. (1402). Morin provides therapeutic effect by attenuating oxidative stress, inflammation, endoplasmic reticulum stress, autophagy, apoptosis, and oxidative DNA damage in testicular toxicity caused by ifosfamide in rats. , 26(10), 1227-1236. doi: 10.22038/ijbms.2023.71702.15580
Fatma Cakmak; Sefa Kucukler; Cihan Gur; Selim Comakli; Mustafa Ileriturk; Fatih Mehmet Kandemir. "Morin provides therapeutic effect by attenuating oxidative stress, inflammation, endoplasmic reticulum stress, autophagy, apoptosis, and oxidative DNA damage in testicular toxicity caused by ifosfamide in rats". , 26, 10, 1402, 1227-1236. doi: 10.22038/ijbms.2023.71702.15580
Cakmak, Fatma, Kucukler, Sefa, Gur, Cihan, Comakli, Selim, Ileriturk, Mustafa, Kandemir, Fatih. (1402). 'Morin provides therapeutic effect by attenuating oxidative stress, inflammation, endoplasmic reticulum stress, autophagy, apoptosis, and oxidative DNA damage in testicular toxicity caused by ifosfamide in rats', , 26(10), pp. 1227-1236. doi: 10.22038/ijbms.2023.71702.15580
Cakmak, Fatma, Kucukler, Sefa, Gur, Cihan, Comakli, Selim, Ileriturk, Mustafa, Kandemir, Fatih. Morin provides therapeutic effect by attenuating oxidative stress, inflammation, endoplasmic reticulum stress, autophagy, apoptosis, and oxidative DNA damage in testicular toxicity caused by ifosfamide in rats. , 1402; 26(10): 1227-1236. doi: 10.22038/ijbms.2023.71702.15580

Morin provides therapeutic effect by attenuating oxidative stress, inflammation, endoplasmic reticulum stress, autophagy, apoptosis, and oxidative DNA damage in testicular toxicity caused by ifosfamide in rats

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 26، شماره 10، دی 2023، صفحه 1227-1236    اصل مقاله (1.45 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71702.15580
نویسندگان
Fatma Cakmak1؛ Sefa Kucukler2؛ Cihan Gur* 2؛ Selim Comakli3؛ Mustafa Ileriturk4؛ Fatih Mehmet Kandemir5
1Private Buhara Hospital, Emergency Medicine Clinic, Erzurum, Turkey
2Atatürk University, Faculty of Veterinary Medicine, Department of Biochemistry, Erzurum, Turkey
3Atatürk University, Faculty of Veterinary Medicine, Department of Pathology, Erzurum, Turkey
4Atatürk University, Horasan Vocational College, Department of Animal Science, Erzurum, Turkey
5Aksaray University, Faculty of Medicine, Department of Medical Biochemistry, Aksaray, Turkey
چکیده
Objective(s): In the present study, it was evaluated whether morin has a protective effect on testicular toxicity caused by ifosfamide (IFOS), which is used in the treatment of various malignancies. 
Materials and Methods: For this purpose, 100 or 200 mg/kg morin was given to Sprague Dawley rats for 2 days, and a single dose (500 mg/kg) IFOS was administered on the 2nd day. At the 24th hr of IFOS administration, animals were decapitated and testicular tissues were taken and the status of oxidative stress, inflammation, endoplasmic reticulum stress (ERS), autophagy, and apoptosis markers were analyzed by biochemical, molecular, and histopathological methods.
Results: According to the data obtained, it was determined that IFOS caused oxidative stress in testicular tissues. It was observed that inflammation, ERS, autophagy, apoptosis, and oxidative DNA damage occurred with oxidative stress. Morin treatment suppressed oxidative stress. Morin showed anti-inflammatory effects by reducing TNF-α and IL-1β protein levels. It also increased the mRNA transcript levels of the ERS marker ATF-6, PERK, IRE1, GRP-78, and CHOP genes, and the apoptosis marker genes Bax, Casp-3, and apaf-1. It up-regulated the anti-apoptotic protein Bcl-2 gene and the cell survival signal AKT-2 gene. Morin caused a decrease in beclin-1 protein levels and showed an anti-autophagic effect. In addition, morin attenuated oxidative DNA damage and decreased 8-OHdG immune-positive cell numbers.
Conclusion: As a result, it was observed that IFOS caused cellular damage by activating various signaling pathways in testicular tissue, while morin exhibited protective properties against this damage.
کلیدواژه‌ها
Apoptosis؛ Ifosfamide؛ Inflammation؛ Morin؛ Oxidative stress؛ Testicular toxicity
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