Expression of Galectin-9-related immune checkpoint receptors in B-cell acute lymphoblastic leukemia | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 12، دوره 26، شماره 12، اسفند 2023، صفحه 1468-1474 اصل مقاله (696.1 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.73159.15901 | ||
| نویسندگان | ||
| Armin Akbar1؛ Hossein asgariyan-Omran1، 2؛ Reza Valadan1، 2؛ Mohammad-Mehdi Dindarloo3؛ Ahmad Najafi1؛ Amir Kahrizi1؛ Arash Poursheikhani4، 5؛ Hossein Karami6، 7؛ Mohammad Naderi6، 7؛ Shayan Sabeti1؛ Mohsen Tehrani* 1، 2 | ||
| 1Department of Immunology, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 2Molecular and Cell-Biology Research Center, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 3Department of Biostatistics and Epidemiology, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 4Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran | ||
| 5Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| 6Department of Hematology and Oncology, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran | ||
| 7Thalassemia Research Center (TRC), Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran | ||
| چکیده | ||
| Objective(s): Exhausted CD8+ T-cells over-express immune checkpoint receptors (ICRs), which interact with their ligands on malignant cells. However, some ICRs have been reported to be expressed on both T-cells and tumor cells, including V-domain immunoglobulin suppressor of T cell activation (VISTA), Galectin-9, and T-cell immunoglobulin mucin-3 (TIM-3). We aimed to evaluate the mRNA expression of VISTA, Galectin-9, and TIM-3 on CD8+ T-cells and leukemic cells in B-cell acute lymphoblastic leukemia (B-ALL). Materials and Methods: Samples were obtained from 26 untreated B-ALL patients and 25 control subjects. CD8+ T-cells were isolated using Magnetic Activated Cell Sorting (MACS). Relative gene expression was then evaluated by qRT-PCR with specific primers for VISTA, Galectin-9, and TIM-3. Also, the mRNA expression profile and clinical data of 154 B-ALL patients were obtained from the TARGET. Results: mRNA expression of Galectin-9 on CD8+ T-cells in B-ALL patients was significantly lower than those in the control group (P=0.043), while VISTA expression was not significantly different between the two study groups (P=0.259). Besides, TIM-3 expression was significantly higher in B-ALL patients than in the control group (P<0.001). Also, data obtained from TARGET showed that the relapse incidence was not significantly different between patients with high and low expression of Galectin-9 and TIM-3 in leukemic cells (P=0.360 and P=0.655, respectively). Conclusion: Collectively, gene expression results suggest an important role for TIM-3, but not VISTA and Galectin-9, in B-ALL and it seems that TIM-3 could be a candidate for immune checkpoint therapy. | ||
| کلیدواژهها | ||
| Acute lymphoblastic - leukemia؛ Gelectin-9؛ HAVCR2؛ Immune checkpoint - receptor؛ T-cell exhaustion؛ TIM-3؛ VISTA | ||
| مراجع | ||
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