سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Urooj, Shaista, Mirani, Zulfiqar, Ahmed Pathan, Faraz, Mustafa, Ghulam, Aziz, Mubashir, Jabeen, Bushra, Shah, Sayed, Ullah, Asad, Hassan, Najmul, Khan, Muhammad Naseem, Raza Rajpoot, Yasir. (1402). Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms. , 26(12), 1475-1483. doi: 10.22038/ijbms.2023.71088.15441
Shaista Urooj; Zulfiqar Ali Mirani; Faraz Ahmed Pathan; Ghulam Mustafa; Mubashir Aziz; Bushra Jabeen; Sayed Hajan Shah; Asad Ullah; Najmul Hassan; Muhammad Naseem Khan; Yasir Raza Rajpoot. "Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms". , 26, 12, 1402, 1475-1483. doi: 10.22038/ijbms.2023.71088.15441
Urooj, Shaista, Mirani, Zulfiqar, Ahmed Pathan, Faraz, Mustafa, Ghulam, Aziz, Mubashir, Jabeen, Bushra, Shah, Sayed, Ullah, Asad, Hassan, Najmul, Khan, Muhammad Naseem, Raza Rajpoot, Yasir. (1402). 'Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms', , 26(12), pp. 1475-1483. doi: 10.22038/ijbms.2023.71088.15441
Urooj, Shaista, Mirani, Zulfiqar, Ahmed Pathan, Faraz, Mustafa, Ghulam, Aziz, Mubashir, Jabeen, Bushra, Shah, Sayed, Ullah, Asad, Hassan, Najmul, Khan, Muhammad Naseem, Raza Rajpoot, Yasir. Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms. , 1402; 26(12): 1475-1483. doi: 10.22038/ijbms.2023.71088.15441

Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 26، شماره 12، اسفند 2023، صفحه 1475-1483    اصل مقاله (636.42 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71088.15441
نویسندگان
Shaista Urooj1، 2؛ Zulfiqar Ali Mirani* 3؛ Faraz Ahmed Pathan4؛ Ghulam Mustafa5؛ Mubashir Aziz6؛ Bushra Jabeen7؛ Sayed Hajan Shah8؛ Asad Ullah3؛ Najmul Hassan9؛ Muhammad Naseem Khan3؛ Yasir Raza Rajpoot2
1Aquatic Diagnostic and Research Center Bahria University Karachi 75260 Pakistan
2Department of Microbiology, University of Karachi, Pakistan
3Microbiology Section, PCSIR Laboratories Complex Karachi-75280 Pakistan
4College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China
5Centre for Development of Laboratory Equipment, PCSIR Laboratories Complex Karachi-75280 Pakistan
6Institute of Microbiology and Molecular Biology, Bahauddin Zakariya University, Multan, Pakistan
7Department of Prosthodontics, Dow International Dental Collage (DUHS) Karachi, Sindh-Pakistan
8Department of Biological Sciences, Government Degree Collage Larkana, Sindh-Pakistan
9Department of Physics and Oxide Research Center, Hankuk University of Foreign Studies, Yongin, 17035, Republic of Korea
چکیده
Objective(s): The current study aimed to investigate the control and treatment of biofilm-producing isolates of Pseudomonas aeruginosa using silicon nanoparticles (SiNPs). 
Materials and Methods: Biofilm-producing isolates of P. aeruginosa were recovered from various food samples and identified through fluorescent green colony formation on selective and differential media, as well as the amplification of oprI and oprL genes. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilm phenotypes. The effect of SiNPs was evaluated by broth dilution assay.
Results: The biofilm assay revealed that these isolates formed biofilms on glass surfaces within 72 hr of incubation. Scanning electron micrographs showed that the biofilm communities were composed of multicellular clusters of P. aeruginosa encased in matrix material. However, these isolates were unable to form biofilms on SiNPs-coated surfaces. The results showed that the planktonic isolates of P. aeruginosa were comparatively sensitive to the antibacterial properties of SiNPs, with minimum inhibitory concentration (MIC) ranging from 100 to 200 µg/ml. Contrarily, the biofilms were found to be 500 times more tolerant to the highest concentration of SiNPs (MIC of 500 µg/ml) and were more resistant. Under static conditions, the sedimentation of SiNPs resulted in their ineffectiveness. However, under shaking conditions, the biofilms were effectively dispersed and the cells were lysed. The results showed that SiNPs were effective against both the planktonic and the metabolically inactive forms of P. aeruginosa.
Conclusion: This study suggests that SiNPs could be a useful tool for preventing the formation of biofilms and removing pre-existing biofilms.
کلیدواژه‌ها
Biofilms؛ Nanoparticles؛ Pseudomonas aeruginosa؛ Silicon؛ SiNPs
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