سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Hanafi, Noorul Izzati, Mohamed, Maizan, Sirajudeen, Kuttulebbai Naina Mohamed Salam, Saidan, Noor Hafizoh, Hua, Gan Siew, Abdul Kadir Pahirulzaman, Khomaizon, Rao, Pasupuleti Visweswara. (1402). Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes. , 26(11), 1360-1369. doi: 10.22038/ijbms.2023.71521.15547
Noorul Izzati Hanafi; Maizan Mohamed; Kuttulebbai Naina Mohamed Salam Sirajudeen; Noor Hafizoh Saidan; Gan Siew Hua; Khomaizon Abdul Kadir Pahirulzaman; Pasupuleti Visweswara Rao. "Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes". , 26, 11, 1402, 1360-1369. doi: 10.22038/ijbms.2023.71521.15547
Hanafi, Noorul Izzati, Mohamed, Maizan, Sirajudeen, Kuttulebbai Naina Mohamed Salam, Saidan, Noor Hafizoh, Hua, Gan Siew, Abdul Kadir Pahirulzaman, Khomaizon, Rao, Pasupuleti Visweswara. (1402). 'Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes', , 26(11), pp. 1360-1369. doi: 10.22038/ijbms.2023.71521.15547
Hanafi, Noorul Izzati, Mohamed, Maizan, Sirajudeen, Kuttulebbai Naina Mohamed Salam, Saidan, Noor Hafizoh, Hua, Gan Siew, Abdul Kadir Pahirulzaman, Khomaizon, Rao, Pasupuleti Visweswara. Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes. , 1402; 26(11): 1360-1369. doi: 10.22038/ijbms.2023.71521.15547

Promoting cardioprotection with fenugreek: Insights from CoCl2-induced hypoxia in neonatal rat cardiomyocytes

Iranian Journal of Basic Medical Sciences
مقاله 14، دوره 26، شماره 11، بهمن 2023، صفحه 1360-1369    اصل مقاله (454.58 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71521.15547
نویسندگان
Noorul Izzati Hanafi1؛ Maizan Mohamed2؛ Kuttulebbai Naina Mohamed Salam Sirajudeen3؛ Noor Hafizoh Saidan1؛ Gan Siew Hua4؛ Khomaizon Abdul Kadir Pahirulzaman* 1؛ Pasupuleti Visweswara Rao* 5، 6، 7
1Faculty of Agro-Based Industry, Universiti Malaysia Kelantan, Jeli, Kelantan, Malaysia
2Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Pengkalan Chepa, Kelantan, Malaysia
3Department of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University Malaysia (IIUM), Bandar Indera Mahkota, Kuantan, Pahang, Malaysia
4School of Pharmacy, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia
5Department of Biomedical Sciences and Therapeutics, Faculty of Medicine and Health Sciences, Universiti Malaysia Sabah, Kota Kinabalu, 88400, Sabah, Malaysia
6Department of Biochemistry, Faculty of Medicine and Health Sciences, Abdurrab University, Pekanbaru, Riau, Indonesia
7Centre for International Relations and Research Collaborations, Reva University, Rukmini Knowledge Park, Kattigenahalli, Yelahanka, Bangalore, 560064, Karnataka, India
چکیده
Objective(s): This study aimed to investigate the protective effects of fenugreek on CoCl2-induced hypoxia in neonatal rat cardiomyocytes.
Materials and Methods: Primary cardiomyocytes were isolated from Sprague Dawley rats aged 0–2 days and incubated with various concentrations of fenugreek (10-320 µg/ml) and CoCl2-induced hypoxia for different durations (24, 48, and 72 hr). Cell viability, calcium signaling, beating rate, and gene expression were evaluated. 
Results: Fenugreek treatments did not cause any toxicity in cardiomyocytes. At a concentration of 160 µg/ml for 24 hr, fenugreek protected the heart against CoCl2-induced hypoxia, as evidenced by reduced expression of caspases (-3, -6, -8, and -9) and other functional genes markers, such as HIF-1α, Bcl-2, IP3R, ERK5, and GLP-1r. Calcium signaling and beating rate were also improved in fenugreek-treated cardiomyocytes. In contrast, CoCl2 treatment resulted in up-regulation of the hypoxia gene HIF-1α and apoptotic caspases gene (-3, -9, -8, -12), and down-regulation of Bcl-2 activity.
Conclusion: Fenugreek treatment at a concentration of 160 µg/ml was not toxic to neonatal rat cardiomyocytes and protected against CoCl2-induced hypoxia. Furthermore, fenugreek improved calcium signaling and beating rate and altered gene expression. Fenugreek may be a potential therapeutic agent for promoting cardioprotection against hypoxia-induced injuries.
کلیدواژه‌ها
Cardiomyocytes؛ Hypoxia؛ Ischemia؛ Therapeutics؛ Trigonella foenum-graecum
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