سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Noori, Tayebeh, Dehpour, Ahmad Reza, Alavi, Seyede Darya, Hosseini, Seyede Zahra, Korani, Sina, Sureda, Antoni, Esmaili, Jamileh, Shirooie, Samira. (1402). Synthesis and evaluation of the effects of solid lipid nanoparticles of ivermectin and ivermectin on cuprizone-induced demyelination via targeting the TRPA1/NF-kB/GFAP signaling pathway. , 26(11), 1272-1282. doi: 10.22038/ijbms.2023.71309.15493
Tayebeh Noori; Ahmad Reza Dehpour; Seyede Darya Alavi; Seyede Zahra Hosseini; Sina Korani; Antoni Sureda; Jamileh Esmaili; Samira Shirooie. "Synthesis and evaluation of the effects of solid lipid nanoparticles of ivermectin and ivermectin on cuprizone-induced demyelination via targeting the TRPA1/NF-kB/GFAP signaling pathway". , 26, 11, 1402, 1272-1282. doi: 10.22038/ijbms.2023.71309.15493
Noori, Tayebeh, Dehpour, Ahmad Reza, Alavi, Seyede Darya, Hosseini, Seyede Zahra, Korani, Sina, Sureda, Antoni, Esmaili, Jamileh, Shirooie, Samira. (1402). 'Synthesis and evaluation of the effects of solid lipid nanoparticles of ivermectin and ivermectin on cuprizone-induced demyelination via targeting the TRPA1/NF-kB/GFAP signaling pathway', , 26(11), pp. 1272-1282. doi: 10.22038/ijbms.2023.71309.15493
Noori, Tayebeh, Dehpour, Ahmad Reza, Alavi, Seyede Darya, Hosseini, Seyede Zahra, Korani, Sina, Sureda, Antoni, Esmaili, Jamileh, Shirooie, Samira. Synthesis and evaluation of the effects of solid lipid nanoparticles of ivermectin and ivermectin on cuprizone-induced demyelination via targeting the TRPA1/NF-kB/GFAP signaling pathway. , 1402; 26(11): 1272-1282. doi: 10.22038/ijbms.2023.71309.15493

Synthesis and evaluation of the effects of solid lipid nanoparticles of ivermectin and ivermectin on cuprizone-induced demyelination via targeting the TRPA1/NF-kB/GFAP signaling pathway

Iranian Journal of Basic Medical Sciences
مقاله 3، دوره 26، شماره 11، بهمن 2023، صفحه 1272-1282    اصل مقاله (632.35 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71309.15493
نویسندگان
Tayebeh Noori1؛ Ahmad Reza Dehpour2، 3؛ Seyede Darya Alavi4؛ Seyede Zahra Hosseini4؛ Sina Korani4؛ Antoni Sureda5، 6؛ Jamileh Esmaili2؛ Samira Shirooie* 1
1Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
2Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3Experimental medicine research center, Tehran University of medical sciences, Tehran, Iran
4Student Research Committee, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran
5Research Group on Community Nutrition and Oxidative Stress (NUCOX) and Health Research Institute of Balearic Islands (IdISBa), University of Balearic Islands-IUNICS, Palma de Mallorca E-07122, Balearic Islands, Spain
6CIBER Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (ISCIII), 28029 Madrid
چکیده
Objective(s): Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS) and its cause is unknown. Several environmental and genetic factors may have roles in the pathogenesis of MS. The synthesis of solid lipid nanoparticles (SLNs) for ivermectin (IVM) loading was performed to increase its efficiency and bioavailability and evaluate its ability in improving the behavioral and histopathological changes induced by cuprizone (CPZ) in the male C57BL/6 mice.   
Materials and Methods: Four groups of 7 adult C57BL/6 mice including control (normal diet), CPZ, IVM, and nano-IVM groups were chosen. After synthesis of nano-ivermectin, demyelination was induced by adding 0.2% CPZ to animal feed for 6 weeks. IVM and nano-IVM (1 mg/kg/day, IP) were given for the final 14 days of the study. At last, behavioral tests, histochemical assays, and immunohistochemistry of TRPA1, NF-kB p65, and GFAP were done.
Results: The time of immobility of mice in the IVM and nano-IVM groups was reduced compared to the CPZ group. Histopathological examination revealed demyelination in the CPZ group, which was ameliorated by IVM and nano-IVM administration. In IVM and nano-IVM groups corpus callosum levels of TRPA1, NF-kB p65, and GFAP were decreased compared to the CPZ group. In the IVM and nano-IVM groups, the levels of MBP were significantly higher than in the CPZ group. 
Conclusion: The results evidenced that IVM and nano-IVM administration is capable of reducing demyelination in mice.
کلیدواژه‌ها
Cuprizone؛ Demyelinating diseases؛ Inflammation؛ Ivermectin؛ Multiple sclerosis؛ Nano-Ivermectin؛ solid lipid nanoparticle؛ TRPA1 cation channel
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