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Altintas, Fatih, Caliskan, Sadettin, Tunc-Ata, Melek, Kilic-Toprak, Emine, Tokgun, Onur, Avci, Neslihan, Kucukatay, Vural. (1402). Investigation of fructose consumption on hippocampal insulin and glucagon-like peptide-1 receptors, and metabolic effects in rats. , 26(11), 1265-1271. doi: 10.22038/ijbms.2023.70711.15369
Fatih Altintas; Sadettin Caliskan; Melek Tunc-Ata; Emine Kilic-Toprak; Onur Tokgun; Neslihan Esra Avci; Vural Kucukatay. "Investigation of fructose consumption on hippocampal insulin and glucagon-like peptide-1 receptors, and metabolic effects in rats". , 26, 11, 1402, 1265-1271. doi: 10.22038/ijbms.2023.70711.15369
Altintas, Fatih, Caliskan, Sadettin, Tunc-Ata, Melek, Kilic-Toprak, Emine, Tokgun, Onur, Avci, Neslihan, Kucukatay, Vural. (1402). 'Investigation of fructose consumption on hippocampal insulin and glucagon-like peptide-1 receptors, and metabolic effects in rats', , 26(11), pp. 1265-1271. doi: 10.22038/ijbms.2023.70711.15369
Altintas, Fatih, Caliskan, Sadettin, Tunc-Ata, Melek, Kilic-Toprak, Emine, Tokgun, Onur, Avci, Neslihan, Kucukatay, Vural. Investigation of fructose consumption on hippocampal insulin and glucagon-like peptide-1 receptors, and metabolic effects in rats. , 1402; 26(11): 1265-1271. doi: 10.22038/ijbms.2023.70711.15369

Investigation of fructose consumption on hippocampal insulin and glucagon-like peptide-1 receptors, and metabolic effects in rats

Iranian Journal of Basic Medical Sciences
مقاله 2، دوره 26، شماره 11، بهمن 2023، صفحه 1265-1271    اصل مقاله (488.68 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.70711.15369
نویسندگان
Fatih Altintas* 1؛ Sadettin Caliskan2؛ Melek Tunc-Ata1؛ Emine Kilic-Toprak1؛ Onur Tokgun3؛ Neslihan Esra Avci4؛ Vural Kucukatay1
1Department of Physiology, Faculty of Medicine, Pamukkale University, Denizli, Turkey
2Department of Physiology, Faculty of Medicine, Üsküdar University, İstanbul, Turkey
3Department of Medical Genetics, Faculty of Medicine, Pamukkale University, Denizli, Turkey
4Department of Physiology, Faculty of Medicine, İzmir Demokrasi University, İzmir, Turkey
چکیده
Objective(s): The detrimental effects of high fructose consumption on metabolic health have been extensively studied. However, limited research has focused on the impact of fructose intake on neuroprotective mechanisms, specifically the expression of insulin receptor (INSR) and glucagon-like peptide-1 receptor (GLP-1R) in the hippocampus. Understanding the effects of fructose on these neuroprotective molecules can provide valuable insights into the potential role of fructose in hippocampal dysfunction. The goal of this study is to aim at the basal plasma levels of lipid profile, insulin, GLP-1, and HOMA-IR, as well as the mRNA and protein expression of neuroprotective molecules such as INSR and GLP-1R in Wistar rats fed a high fructose diet. 
Materials and Methods: Rats were separated into control (C) and high fructose (HF) groups. The HF group was given 20% fructose water to drink for 16 weeks. 
Results: Fructose ingestion significantly increased abdominal fat (C=1.24±0.08 g, HF=1.79±0.19 g, P<0.05) and plasma triglyceride levels (C=179.22±22.85 µg/ml, HF=242.45±14.45 µg/ml, P<0.05), but had no statistically significant effect on body weight and plasma HDL, LDL, total cholesterol, insulin, and GLP-1 levels (P>0.05). Although INSR mRNA expression in the hippocampus was significantly lower in the HF group compared to the control group (P<0.05), GLP-1R mRNA expression did not differ significantly across the groups (P>0.05). Furthermore, whereas INSR and GLP-1R protein levels in the experimental group were on a declining trend, this trend was not substantially different (P>0.05).
Conclusion: These data suggest that fructose consumption may be harmful to the hippocampus by lowering the expression of INSR.
کلیدواژه‌ها
Fructose؛ Glucagon-like peptide-1, Hippocampus؛ Insulin؛ Lipid profile
مراجع
1. Fidler Mis N, Braegger C, Bronsky J, Campoy C, Domellöf M, Embleton ND, et al. Sugar in infants, children and adolescents: A position paper of the european society for paediatric gastroenterology, hepatology and nutrition committee on nutrition. J Pediatr Gastroenterol Nutr 2017; 65:681-696. 
2. Payant MA, Chee MJ. Neural mechanisms underlying the role of fructose in overfeeding. Neurosci Biobehav Rev 2021; 128:346-357. 
3. Dornas WC, de Lima WG, Pedrosa ML, and Silva ME. Health implications of high-fructose intake and current research. Adv Nutr 2015;6:729-737. 
4. Kovačević S, Nestorov J, Matić G, Elaković I. Dietary fructose-related adiposity and glucocorticoid receptor function in visceral adipose tissue of female rats. Eur J Nutr 2014; 53:1409-1420. 
5. Prince PD, Santander YA, Gerez EM, Höcht C, Polizio AH, Mayer MA, et al. Fructose increases corticosterone production in association with NADPH metabolism alterations in rat epididymal white adipose tissue. J Nutr Biochem 2017; 46:109-116. 
6. Lustig RH, Schmidt LA, Brindis CD. Public health: The toxic truth about sugar. Nature 2012; 482:27-29. 
7. Tappy L, Lê KA. Health effects of fructose and fructose-containing caloric sweeteners: Where do we stand 10 years after the initial whistle blowings? Curr Diab Rep 2015; 15:54-65. 
8. Luger M, Lafontan M, Bes-Rastrollo M, Winzer E, Yumuk V, Farpour-Lambert N. Sugar-sweetened beverages and weight gain in children and adults: A systematic review from 2013 to 2015 and a comparison with previous studies. Obes Facts 2018; 10:674-693. 
9. Hu FB, Malik VS. Sugar-sweetened beverages and risk of obesity and type 2 diabetes: Epidemiologic evidence. Physiol Behav 2010; 100:47-54. 
10. Yang Q, Zhang Z, Gregg EW, Flanders WD, Merritt R, Hu FB. Added sugar intake and cardiovascular diseases mortality among us adults. JAMA Intern Med 2014; 174:516-524. 
11. Rippe JM, Angelopoulos TJ. Sucrose, high-fructose corn syrup, and fructose, their metabolism and potential health effects: What do we really know? Adv Nutr 2013; 4:236-245. 
12. Jiménez-Maldonado A, Ying Z, Byun HR, Gomez-Pinilla F. Short-term fructose ingestion affects the brain independently from establishment of metabolic syndrome. Biochim Biophys Acta Mol Basis Dis 2018; 1864:24-33. 
13. Liu WC, Wu CW, Tain YL, Fu MH, Hung CY, Chen IC, et al. Oral pioglitazone ameliorates fructose-induced peripheral insulin resistance and hippocampal gliosis but not restores inhibited hippocampal adult neurogenesis. Biochim Biophys Acta Mol Basis Dis 2018; 1864:274-285. 
14. Cisternas P, Salazar P, Serrano FG, Montecinos-Oliva C, Arredondo SB, Varela-Nallar L, et al. Fructose consumption reduces hippocampal synaptic plasticity underlying cognitive performance. Biochim Biophys Acta Mol Basis Dis 2015; 1852:2379-2390. 
15. Alvarez E, Martínez MD, Roncero I, Chowen JA, García-Cuartero B, Gispert JD, et al. The expression of GLP-1 receptor mRNA and protein allows the effect of GLP-1 on glucose metabolism in the human hypothalamus and brainstem. J Neurochem 2005; 92:798-806. 
16. During MJ, Cao L, Zuzga DS, Francis JS, Fitzsimons HL, Jiao X, et al. Glucagon-like peptide-1 receptor is involved in learning and neuroprotection. Nat Med 2003; 9:1173-1179. 
17. Hölscher C, Li L. New roles for insulin-like hormones in neuronal signalling and protection: New hopes for novel treatments of Alzheimer’s disease? Neurobiol Aging 2010; 31:1495-1502. 
18. Abbas T, Faivre E, and Hölscher C. Impairment of synaptic plasticity and memory formation in GLP-1 receptor KO mice: Interaction between type 2 diabetes and Alzheimer’s disease. Behav Brain Res 2009; 205:265-271. 
19. Pomytkin I, Costa-Nunes JP, Kasatkin V, Veniaminova E, Demchenko A, Lyundup A, et al. Insulin receptor in the brain: Mechanisms of activation and the role in the CNS pathology and treatment. CNS Neurosci Ther 2018; 24:763-774. 
20. Batista AF, Forny-Germano L, Clarke JR, Lyra e Silva NM, Brito-Moreira J, Boehnke SE, et al. The diabetes drug liraglutide reverses cognitive impairment in mice and attenuates insulin receptor and synaptic pathology in a non-human primate model of Alzheimer’s disease. J Pathol 2018; 245:85-100. 
21. Candeias E, Sebastião I, Cardoso S, Carvalho C, Santos MS, Oliveira CR, et al. Brain GLP-1/IGF-1 signaling and autophagy mediate exendin-4 protection against apoptosis in type 2 diabetic rats. Mol Neurobiol 2018; 55:4030-4050. 
22. Stanišić J, Korićanac G, Ćulafić T, Romić S, Stojiljković M, Kostić M, et al. Low intensity exercise prevents disturbances in rat cardiac insulin signaling and endothelial nitric oxide synthase induced by high fructose diet. Mol Cell Endocrinol 2016; 420:97-104. 
23. Mamikutty N, Thent ZC, Sapri SR, Sahruddin NN, Mohd Yusof MR, Haji Suhaimi F. The establishment of metabolic syndrome model by induction of fructose drinking water in male Wistar rats. Biomed Res Int 2014; 2014:263897-263904. 
24. Dai S, McNeill JH. Fructose-induced hypertension in rats is concentration-and duration-dependent. J Pharmacol Toxicol Methods 1995; 33:101-107. 
25. Azarang A, Farshad O, Ommati MM, Jamshidzadeh A, Heidari R, Abootalebi SN, et al. Protective role of probiotic supplements in hepatic steatosis: A rat model study. Biomed Res Int 2020; 2020:5487659-5487673. 
26. Swarbrick MM, Stanhope KL, Elliott SS, Graham JL, Krauss RM, Christiansen MP, et al. Consumption of fructose-sweetened beverages for 10 weeks increases postprandial triacylglycerol and apolipoprotein-B concentrations in overweight and obese women. Br J Nutr 2008;100:947-952. 
27. Czerwonogrodzka-Senczyna A, Rumińska M, Majcher A, Credo D, Jeznach-Steinhagen A, Pyrżak B. Fructose consumption and lipid metabolism in obese children and adolescents. Adv Exp Med Biol 2019; 1153:91-100. 
28. Sigala DM, Hieronimus B, Medici V, Lee V, Nunez Mv., Bremer AA, et al. Consuming sucrose- or HFCS-sweetened beverages increases hepatic lipid and decreases insulin sensitivity in adults. J Clin Endocrinol Metab 2021; 106:3248-3264. 
29. Agame-Lagunes B, Grube-Pagola P, García-Varela R, Alexander-Aguilera A, García HS. Effect of curcumin nanoemulsions stabilized with MAG and DAG-MCFAs in a fructose-induced hepatic steatosis rat model. Pharmaceutics 2021;13:509-524. 
30. Sunehag AL, Toffolo G, Campioni M, Bier DM, Haymond MW. Short-term high dietary fructose intake had no effects on insulin sensitivity and secretion or glucose and lipid metabolism in healthy, obese adolescents. J Pediatr Endocrinol Metab 2008; 21:225-235. 
31. Bantle JP, Raatz SK, Thomas W, and Georgopoulos A. Effects of dietary fructose on plasma lipids in healthy subjects. Am J Clin Nutr 2000; 72:1128-1134. 
32. Gilijamse PW, Demirkiran A, van Wagensveld BA, Ackermans MT, Romijn JA, Nieuwdorp M, et al. The relation between postprandial glucagon-like peptide-1 release and insulin sensitivity before and after bariatric surgery in humans with class II/III obesity. Surg Obes Relat Dis 2021; 17:1440-1448. 
33. Hölscher C. Protective properties of GLP-1 and associated peptide hormones in neurodegenerative disorders. Br J Pharmacol 2022; 179:695-714. 
34. Treviño S, Aguilar-Alonso P, Flores Hernandez JA, Brambila E, Guevara J, Flores G, et al. A high calorie diet causes memory loss, metabolic syndrome and oxidative stress into hippocampus and temporal cortex of rats. Synapse 2015; 69:421-433. 
35. Djordjevic A, Bursać B, Veličković N, Vasiljević A, Matić G. The impact of different fructose loads on insulin sensitivity, inflammation, and PSA-NCAM-mediated plasticity in the hippocampus of fructose-fed male rats. Nutr Neurosci 2015; 18:66-75. 
36. Agrawal R, Gomez-Pinilla F. ‘Metabolic syndrome’ in the brain: deficiency in omega-3 fatty acid exacerbates dysfunctions in insulin receptor signalling and cognition. J Physiol 2012; 590:2485-2499. 
37. Calvo-Ochoa E, Hernández-Ortega K, Ferrera P, Morimoto S, Arias C. Short-term high-fat-and-fructose feeding produces insulin signaling alterations accompanied by neurite and synaptic reduction and astroglial activation in the rat hippocampus. J Cereb Blood Flow Metab 2014; 34:1001-1008. 
38. Spagnuolo MS, Iossa S, Cigliano L. Sweet but bitter: Focus on fructose impact on brain function in rodent models. Nutrients 2021; 13:1-18. 
39. Mielke JG, Taghibiglou C, Liu L, Zhang Y, Jia Z, Adeli K, et al. A biochemical and functional characterization of diet-induced brain insulin resistance. J Neurochem 2005; 93:1568-1578. 
40. Perl K, Ushakov K, Pozniak Y, Yizhar-Barnea O, Bhonker Y, Shivatzki S, et al. Reduced changes in protein compared to mRNA levels across non-proliferating tissues. BMC Genomics 2017;18:305-319. 
41. Nie L, Wu G, Zhang W. Correlation of mRNA Expression and protein abundance affected by multiple sequence features related to translational efficiency in desulfovibrio vulgaris: A quantitative analysis. Genetics 2006; 174:2229-2243. 
42. Perry T, Haughey NJ, Mattson MP, Egan JM, Greig NH. Protection and reversal of excitotoxic neuronal damage by glucagon-like peptide-1 and exendin-4. J Pharmacol Exp Ther 2002; 302:881-888. 
43. Perry TA, Lahiri DK, Sambamurti K, Chen D, Mattson MP, Egan JM, et al. Glucagon-like peptide-1 decreases endogenous amyloid-β peptide (Aβ) levels and protects hippocampal neurons from death induced by Aβ and iron. J Neurosci Res 2003; 72:603-612. 
44. Bertilsson G, Patrone C, Zachrisson O, Andersson A, Dannaeus K, Heidrich J, et al. Peptide hormone exendin-4 stimulates subventricular zone neurogenesis in the adult rodent brain and induces recovery in an animal model of Parkinson’s disease. J Neurosci Res 2008; 86:326-338. 
45. Diz-Chaves Y, Herrera-Pérez S, González-Matías LC, Mallo F. Effects of Glucagon-like peptide 1 (GLP-1) analogs in the hippocampus. Vitam Horm 2022; 118:457-478. 
46. Stranahan AM, Norman ED, Lee K, Cutler RG, Telljohann RS, Egan JM, et al. Diet-induced insulin resistance impairs hippocampal synaptic plasticity and cognition in middle-aged rats. Hippocampus 2008; 18:1085-1088. 

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