Mucosal and systemic immunization against tuberculosis by ISCOMATRIX nano adjuvant co-administered with alginate coated chitosan nanoparticles | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 6، دوره 26، شماره 10، دی 2023، صفحه 1162-1167 اصل مقاله (263.43 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2023.67564.14806 | ||
| نویسندگان | ||
| Adel Najafi1؛ Kiarash Ghazvini2؛ Mojtaba Sankian3؛ Leila Gholami4؛ Sirvan Zare3؛ Arshid Youdefi-Avarvand5؛ Mohsen Tafaghodi* 6 | ||
| 1Clinical Microbiology Laboratory, Fatemieh hospital, Hamadan University of Medical Science, Hamedan, Iran | ||
| 2Department of Microbiology, Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran | ||
| 3Immunology Research Center, Bu Ali Research Institute, Mashhad University of Medical Science, Mashhad, Iran | ||
| 4Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran | ||
| 5Department of Laboratory Sciences, School of Allied Medical Sciences, Ahavz Jundishapur University of Medical Sciences, Ahvaz, Iran | ||
| 6Nanotechnology Research center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran | ||
| چکیده | ||
| Objective(s): BCG vaccine has no longer been appreciated to immunize against tuberculosis, worldwide, so novel appropriate adjuvants have been dedicated to improve immune responses. This study aimed to evaluate the immunomodulatory effects of ISCOMATRIX as an adjuvant to stimulate potent humoral and cellular immune responses of the PPE17 loaded alginate coated nanoparticles through subcutaneous and intranasal vaccination. Materials and Methods: Size, polydispersity index, and morphology of the resulting colloidal particles were explored by dynamic light scattering (DLS). The cellular and/or humoral immune stimulation properties of ISCOMATRIX adjuvant were measured by measuring the level of IFNγ, IL-4, IL-17, and TGFβ in spleen cell cultures and IgG1 and IgG2a in serum and sIgA in nasal lavage of immunized mice, respectively. Results: The spherical cage-like particles of ISCOMATRIX adjuvant have optimal size of 59±6 nm appropriate for an immune adjuvant vaccine. ISCOMATRIX induced robust Th1 (IFN-γ) and IL-17 cytokine response also significant IgG2a and IgG1antibodies in both subcutaneous and intranasal routes and elicited mucosal sIgA response when administered intranasally. As a booster for BCG, ISCOMATRIX induced immune responses only in subcutaneous route. Conclusion: These findings indicate that ISCOMATRIX is a promising adjuvant with the potential for increasing cellular and humoral immunity both after subcutaneous and intranasal administration. | ||
| کلیدواژهها | ||
| Adjuvant؛ Chitosan؛ Immune response؛ ISCOMATRIX؛ Nanoparticle | ||
| مراجع | ||
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