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Javadi, Faezeh, Ale-Ebrahim, Mahsa, Mohseni-Moghaddam, Parvaneh, Mortazavi, Pejman, Mousavi, zahra, Asghari, Ahmad. (1402). Hepatoprotective and antifibrotic effects of trans-chalcone against bile duct ligation-induced liver fibrosis in rats. , 26(10), 1194-1201. doi: 10.22038/ijbms.2023.68342.15040
Faezeh Javadi; Mahsa Ale-Ebrahim; Parvaneh Mohseni-Moghaddam; Pejman Mortazavi; zahra Mousavi; Ahmad Asghari. "Hepatoprotective and antifibrotic effects of trans-chalcone against bile duct ligation-induced liver fibrosis in rats". , 26, 10, 1402, 1194-1201. doi: 10.22038/ijbms.2023.68342.15040
Javadi, Faezeh, Ale-Ebrahim, Mahsa, Mohseni-Moghaddam, Parvaneh, Mortazavi, Pejman, Mousavi, zahra, Asghari, Ahmad. (1402). 'Hepatoprotective and antifibrotic effects of trans-chalcone against bile duct ligation-induced liver fibrosis in rats', , 26(10), pp. 1194-1201. doi: 10.22038/ijbms.2023.68342.15040
Javadi, Faezeh, Ale-Ebrahim, Mahsa, Mohseni-Moghaddam, Parvaneh, Mortazavi, Pejman, Mousavi, zahra, Asghari, Ahmad. Hepatoprotective and antifibrotic effects of trans-chalcone against bile duct ligation-induced liver fibrosis in rats. , 1402; 26(10): 1194-1201. doi: 10.22038/ijbms.2023.68342.15040

Hepatoprotective and antifibrotic effects of trans-chalcone against bile duct ligation-induced liver fibrosis in rats

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 26، شماره 10، دی 2023، صفحه 1194-1201    اصل مقاله (418.09 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68342.15040
نویسندگان
Faezeh Javadi1؛ Mahsa Ale-Ebrahim* 2؛ Parvaneh Mohseni-Moghaddam2؛ Pejman Mortazavi3؛ zahra Mousavi1؛ Ahmad Asghari3
1Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2Department of Physiology, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3Department of Pathology, Faculty of Specialized Veterinary, Science and Research Branch, Islamic Azad University, Tehran, Iran
چکیده
Objective(s): Several lines of research have shown that hepatic fibrosis is one of the leading causes of death worldwide. Trans-chalcone is a flavonoid precursor with anti-oxidant and anti-inflammatory effects. The present study was conducted to examine the antifibrotic properties of trans-chalcone on bile duct ligation (BDL)-induced liver cholestasis in rats. 
Materials and Methods: Following the BDL operation, trans-chalcone at doses of 12, 24, and 50 mg/kg was administered orally once a day for 45 consecutive days. Serum levels of liver indices, including alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total and direct bilirubin, and lipid profile in addition to blood urea nitrogen (BUN) and creatinine, were measured. Additionally, catalase (CAT) and superoxide dismutase (SOD) activities were assessed in liver homogenates. Histopathological evaluations were performed using Masson trichrome (MT) and hematoxylin and eosin (H&E) staining. 
Results: The elevated levels of liver enzymes, total and direct bilirubin, BUN, creatinine, cholesterol, triglyceride, and low-density lipoprotein (LDL) induced by BDL were significantly reduced following trans-chalcone administration; while serum level of high-density lipoprotein (HDL) increased. Besides, treatment with trans-chalcone elevated the activities of CAT and SOD in the liver tissues of the animals with BDL surgery. According to MT and H&E staining, BDL-induced histopathological changes, including infiltration of inflammatory cells, hepatocyte necrosis, ductal hyperplasia, and collagen deposition were ameliorated using trans-chalcone administration. 
Conclusion: It can be concluded from the present study that trans-chalcone, possibly by its anti-oxidant and anti-inflammatory properties, may exert hepatoprotective and antifibrotic effects in BDL-induced liver fibrosis. v
کلیدواژه‌ها
Anti-oxidant؛ Bile duct ligation؛ Cholestasis؛ Hepatic fibrosis؛ Trans-chalcone
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