سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Durairaj, Alwin Beschi, Sivaganam, Ananthi, Monikam, Reginald Appavoo, Krishnamoorthy, Rajapandiyan, Z. Ahmed, Mohammad, Ali S., Alqahtani, Mideen, Ponani Kaja. (1402). Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis. , 26(10), 1202-1211. doi: 10.22038/ijbms.2023.71125.15450
Alwin Beschi Durairaj; Ananthi Sivaganam; Reginald Appavoo Monikam; Rajapandiyan Krishnamoorthy; Mohammad Z. Ahmed; Alqahtani Ali S.; Ponani Kaja Mideen. "Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis". , 26, 10, 1402, 1202-1211. doi: 10.22038/ijbms.2023.71125.15450
Durairaj, Alwin Beschi, Sivaganam, Ananthi, Monikam, Reginald Appavoo, Krishnamoorthy, Rajapandiyan, Z. Ahmed, Mohammad, Ali S., Alqahtani, Mideen, Ponani Kaja. (1402). 'Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis', , 26(10), pp. 1202-1211. doi: 10.22038/ijbms.2023.71125.15450
Durairaj, Alwin Beschi, Sivaganam, Ananthi, Monikam, Reginald Appavoo, Krishnamoorthy, Rajapandiyan, Z. Ahmed, Mohammad, Ali S., Alqahtani, Mideen, Ponani Kaja. Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis. , 1402; 26(10): 1202-1211. doi: 10.22038/ijbms.2023.71125.15450

Deregulation of apoptotic proteins by induction of Dendropthae falcata (L.f.) Ettingsh plant extract in breast cancer cells: A proteome-wide analysis

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 26، شماره 10، دی 2023، صفحه 1202-1211    اصل مقاله (1.01 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.71125.15450
نویسندگان
Alwin Beschi Durairaj* 1؛ Ananthi Sivaganam2، 3؛ Reginald Appavoo Monikam4؛ Rajapandiyan Krishnamoorthy5؛ Mohammad Z. Ahmed6؛ Alqahtani Ali S.6؛ Ponani Kaja Mideen7
1Department of Botany & Research Center, Scott Christian College, Nagercoil – 629003, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627012, Tamil Nadu, India
2Department of Proteomics, Clinbiocare Technology, Tenkasi – 627 814, Tamil Nadu, India
3Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India
4Department of Botany & Research Center, Scott Christian College, Nagercoil – 629003, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627012, Tamil Nadu, India
5Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia
6Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
7Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi, USA
چکیده
Objective(s): The present study evaluated the protein-based analysis to unravel the role and mechanism behind the Dendropthae falcata plant extract treatment in breast cancer cells. 
Materials and Methods: The protein sample was extracted from the cancer cells after treatment with the plant extract and subjected to two-dimensional electrophoresis for protein separation. Further, the proteins that were differentially regulated among the samples which were treated and non-treated were selected and processed further for protein identification using a tandem mass spectrometry approach.
Results: Using these strategies, we identified 16 potential candidates which were showing remarkable changes in treated samples. All the candidates were analyzed further for gene ontology analysis, and it was observed that all proteins were involved in multiple pathways pertaining to the carcinogenesis process. Specifically, apoptotic pathway proteins including BAD, BIK, BID, CASP8, MCL1, BCL2, and BAK1 were highly impacted by treatment with D. falcata plant extract. All these protein hits were further taken for validation experiments using RT PCR analysis. 
Conclusion: Initiation of these apoptotic proteins by D. falcata plant extract treatment in breast cancer cells shows a positive direction toward nature-based alternative medicine.
کلیدواژه‌ها
2D-Gel Electrophoresis؛ Breast cancer cell line؛ Dendropthae falcate؛ Mass spectrometry؛ Medicinal plant؛ Proteomics
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