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Toktay, Erdem, Selli, Jale, Gurbuz, Muhammed, Alaca, Raziye. (1402). Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats. , 26(10), 1155-1161. doi: 10.22038/ijbms.2023.69984.15223
Erdem Toktay; Jale Selli; Muhammed Ali Gurbuz; Raziye Alaca. "Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats". , 26, 10, 1402, 1155-1161. doi: 10.22038/ijbms.2023.69984.15223
Toktay, Erdem, Selli, Jale, Gurbuz, Muhammed, Alaca, Raziye. (1402). 'Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats', , 26(10), pp. 1155-1161. doi: 10.22038/ijbms.2023.69984.15223
Toktay, Erdem, Selli, Jale, Gurbuz, Muhammed, Alaca, Raziye. Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats. , 1402; 26(10): 1155-1161. doi: 10.22038/ijbms.2023.69984.15223

Investigation of the effects of astaxanthin in experimental polycystic ovary syndrome (PCOS) in rats

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 26، شماره 10، دی 2023، صفحه 1155-1161    اصل مقاله (485.43 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.69984.15223
نویسندگان
Erdem Toktay* 1؛ Jale Selli2؛ Muhammed Ali Gurbuz3؛ Raziye Alaca4
1Department of Histology and Embryology, Faculty of Medicine, Kafkas University, Kars, Turkey
2Department of Histology and Embryology, Faculty of Medicine, Alanya Alaaddin Keykubat University, Antalya, Turkey
3Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
4Philosophy Doctor Degree, Department of Histology and Embryology, Erzurum City Hospital, Erurum, Turkey
چکیده
Objective(s): The aim of this study was to investigate the effect of Astaxanthin (ASX) on ovaries in letrozole-induced polycystic ovary syndrome (PCOS) model in female rats by histopathological, immunohistochemical and biochemical techniques.
Materials and Methods: Seventy two Sprague-Dawley female rats with an average weight of 200-250 gr and 10-12 weeks old were randomly divided into 9 groups. PCOS model was applied to all groups except healthy group. In the study, low (10 mg / kg) moderate (20 mg / kg) and high (40 mg / kg) doses of ASX were given to the experimental animals in the PCOS-induced groups for 7 days. At the end of the experiment, ovarian tissues were evaluated histopathologically, immunohistochemically, and biochemically.
Results: When the histopathological findings were examined, many cystic follicles, apoptotic and necrotic cells were found in the follicles in the PCOS group. In addition, significant decrease in apoptotic and necrotic cells were observed in PCOS+MET+ASX and PCOS+ASX groups. In immunohistochemical staining findings, while TNF-α, NF-κB and IL-6 expression levels showed significant increase in PCOS group, these expression levels were decreased in PCOS+MET+ASX and PCOS+ASX groups. In the biochemical evaluations, while MDA were increased, SOD were decreased in the PCOS group. MDA level were decreased while SOD levels were increased in the PCOS+MET+ASX and PCOS+ASX groups. 
Conclusion: In addition to the formation of insulin resistance in the tissue, severe oxidative stress damage occurs in ovarian tissue during PCOS. Metformin improved PCOS by correcting insulin resistance. In this period, the administration of ASX with Metformin protected the ovary from oxidative stress damage.
کلیدواژه‌ها
Astaxanthin؛ Metformin؛ Ovary؛ Oxidative stress؛ polycystic ovary syndrome
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