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Bavarsad, Neda, Hemmati, Aliasghar, Jafarian, Fateme, Mostoufi, Azar, Siahpoosh, Amir, Rashidi Nooshabadi, Mohammadreza, Mansouri, Esrafil. (1402). Preparation and physicochemical characterization of N-succinyl chitosan-coated liposomes for oral delivery of grape seed extract and evaluation of its effect on pulmonary fibrosis induced by bleomycin in rats. , 26(10), 1237-1244. doi: 10.22038/ijbms.2023.70797.15381
Neda Bavarsad; Aliasghar Hemmati; Fateme Jafarian; Azar Mostoufi; Amir Siahpoosh; Mohammadreza Rashidi Nooshabadi; Esrafil Mansouri. "Preparation and physicochemical characterization of N-succinyl chitosan-coated liposomes for oral delivery of grape seed extract and evaluation of its effect on pulmonary fibrosis induced by bleomycin in rats". , 26, 10, 1402, 1237-1244. doi: 10.22038/ijbms.2023.70797.15381
Bavarsad, Neda, Hemmati, Aliasghar, Jafarian, Fateme, Mostoufi, Azar, Siahpoosh, Amir, Rashidi Nooshabadi, Mohammadreza, Mansouri, Esrafil. (1402). 'Preparation and physicochemical characterization of N-succinyl chitosan-coated liposomes for oral delivery of grape seed extract and evaluation of its effect on pulmonary fibrosis induced by bleomycin in rats', , 26(10), pp. 1237-1244. doi: 10.22038/ijbms.2023.70797.15381
Bavarsad, Neda, Hemmati, Aliasghar, Jafarian, Fateme, Mostoufi, Azar, Siahpoosh, Amir, Rashidi Nooshabadi, Mohammadreza, Mansouri, Esrafil. Preparation and physicochemical characterization of N-succinyl chitosan-coated liposomes for oral delivery of grape seed extract and evaluation of its effect on pulmonary fibrosis induced by bleomycin in rats. , 1402; 26(10): 1237-1244. doi: 10.22038/ijbms.2023.70797.15381

Preparation and physicochemical characterization of N-succinyl chitosan-coated liposomes for oral delivery of grape seed extract and evaluation of its effect on pulmonary fibrosis induced by bleomycin in rats

Iranian Journal of Basic Medical Sciences
مقاله 15، دوره 26، شماره 10، دی 2023، صفحه 1237-1244    اصل مقاله (588.5 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.70797.15381
نویسندگان
Neda Bavarsad* 1، 2؛ Aliasghar Hemmati3، 4؛ Fateme Jafarian2؛ Azar Mostoufi5، 6؛ Amir Siahpoosh4، 7؛ Mohammadreza Rashidi Nooshabadi3؛ Esrafil Mansouri8
1Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
2Department of Pharmaceutics, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3Department of Pharmacology, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
4Medicinal Plant Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
5Marine Pharmaceutical Science Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
6Department of Medicinal Chemistry, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
7Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
8Cellular and Molecular Research Center, Department of Anatomical Sciences, Faculty of Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
چکیده
Objective(s): This study aimed to develop an oral succinyl chitosan-coated liposomal formulation containing grape seed extract and assess its therapeutic efficacy in rats with bleomycin-induced pulmonary fibrosis. 
Materials and Methods: N-succinyl chitosan was synthesized, and the liposomal formulations were prepared and characterized regarding phenolic content assay and morphology. Size, zeta potential, in vitro drug release, and stability. Pulmonary fibrosis was induced by intratracheal bleomycin injection, and hydroxyproline measurements, lung weight, animal body weight, as well as histopathological studies were performed
Results: Succinyl chitosan increases the physical stability of the formulation, especially in acidic conditions. Drug release studies revealed that 66.27% of the loaded drug was released from CF2 in an acidic medium in 2 hr, but 92.31% of the drug was released in 8 hr in a pH=7 medium. An in vivo study demonstrated that rats exposed to bleomycin significantly lost weight, while those treated with CF2 (400 mg/kg) partially regained weight. Bleomycin treatment increased the mean lung weight and the amount of hydroxyproline in the lungs; these values were significantly decreased in the group treated with 400 mg/kg CF2 (P<0.05). Histopathological examination confirmed that treatment with 400 mg/kg CF2 improved lung fibrosis. 
Conclusion: In rats, oral administration of N-succinyl chitosan-coated liposomes containing grape seed extract at the 400 mg/kg dose ameliorates bleomycin-induced pulmonary fibrosis.
کلیدواژه‌ها
Bleomycin؛ Grape seed extract؛ Liposomes؛ N-succinyl chitosan؛ Pulmonary fibrosis
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