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Barangi, Samira, Hosseinizadeh, Parisa, Karimi, Gholamreza, Tayarani‑Najaran, Zahra, Mehri, Soghra. (1402). Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways. , 26(8), 953-959. doi: 10.22038/ijbms.2023.68292.14905
Samira Barangi; Parisa Hosseinizadeh; Gholamreza Karimi; Zahra Tayarani‑Najaran; Soghra Mehri. "Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways". , 26, 8, 1402, 953-959. doi: 10.22038/ijbms.2023.68292.14905
Barangi, Samira, Hosseinizadeh, Parisa, Karimi, Gholamreza, Tayarani‑Najaran, Zahra, Mehri, Soghra. (1402). 'Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways', , 26(8), pp. 953-959. doi: 10.22038/ijbms.2023.68292.14905
Barangi, Samira, Hosseinizadeh, Parisa, Karimi, Gholamreza, Tayarani‑Najaran, Zahra, Mehri, Soghra. Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways. , 1402; 26(8): 953-959. doi: 10.22038/ijbms.2023.68292.14905

Osthole attenuated cytotoxicity induced by 6-OHDA in SH-SY5Y cells through inhibition of JAK/STAT and MAPK pathways

Iranian Journal of Basic Medical Sciences
مقاله 13، دوره 26، شماره 8، آبان 2023، صفحه 953-959    اصل مقاله (498.01 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68292.14905
نویسندگان
Samira Barangi1؛ Parisa Hosseinizadeh2؛ Gholamreza Karimi1، 2؛ Zahra Tayarani‑Najaran2، 3؛ Soghra Mehri* 1، 2
1Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
2Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
3Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Natural coumarin called osthole is regarded as a medicinal herb with widespread applications in Traditional Chinese Medicine. It has various pharmacological properties, including antioxidant, anti-inflammatory, and anti-apoptotic effects. In some neurodegenerative diseases, osthole also shows neuroprotective properties. In this study, we explored how osthole protects human neuroblastoma SH-SY5Y cells from the cytotoxicity of 6-hydroxydopamine (6-OHDA). 
Materials and Methods: Using the MTT assay and DCFH-DA methods, respectively, the viability of the cells and the quantity of intracellular reactive oxygen species (ROS) were evaluated. Signal Transducers and Activators of Transcription (STAT), Janus Kinase (JAK), extracellular signal-regulated kinase 1/2 (ERK1/2), c-Jun N-terminal kinase (JNK), and caspase-3 activation levels were examined using western blotting.
Results: In SH-SY5Y cells, the results showed that a 24-hour exposure to 6-OHDA (200 µM) lowered cell viability but markedly elevated ROS, p-JAK/JAK, p-STAT/STAT, p-ERK/ERK, p-JNK/JNK ratio, and caspase-3 levels. Interestingly, osthole (100 µM) pretreatment of cells for 24 hr prevented 6-OHDA-induced cytotoxicity by undoing all effects of 6-OHDA. 
Conclusion: In summary, our data showed that osthole protects SH-SY5Y cells against 6-OHDA-induced cytotoxicity by inhibiting ROS generation and reducing the activity of the JAK/STAT, MAPK, and apoptotic pathways.
کلیدواژه‌ها
6-OHDA؛ Apoptosis؛ JAK Kinases؛ Mitogen-activated protein-kinase؛ Osthole؛ STAT3
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