سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mousavinezhad-Moghaddam, Maryam, Behnam-Rassouli, Morteza, Valizadeh, Narges, Mahdavi-Shahri, Naser, Rezaee, Seyed Abdolrahim. (1402). Thiamine as a peripheral neuro-protective agent in comparison with N-acetyl cysteine in axotomized rats. , 26(8), 919-926. doi: 10.22038/ijbms.2023.67157.14726
Maryam Mousavinezhad-Moghaddam; Morteza Behnam-Rassouli; Narges Valizadeh; Naser Mahdavi-Shahri; Seyed Abdolrahim Rezaee. "Thiamine as a peripheral neuro-protective agent in comparison with N-acetyl cysteine in axotomized rats". , 26, 8, 1402, 919-926. doi: 10.22038/ijbms.2023.67157.14726
Mousavinezhad-Moghaddam, Maryam, Behnam-Rassouli, Morteza, Valizadeh, Narges, Mahdavi-Shahri, Naser, Rezaee, Seyed Abdolrahim. (1402). 'Thiamine as a peripheral neuro-protective agent in comparison with N-acetyl cysteine in axotomized rats', , 26(8), pp. 919-926. doi: 10.22038/ijbms.2023.67157.14726
Mousavinezhad-Moghaddam, Maryam, Behnam-Rassouli, Morteza, Valizadeh, Narges, Mahdavi-Shahri, Naser, Rezaee, Seyed Abdolrahim. Thiamine as a peripheral neuro-protective agent in comparison with N-acetyl cysteine in axotomized rats. , 1402; 26(8): 919-926. doi: 10.22038/ijbms.2023.67157.14726

Thiamine as a peripheral neuro-protective agent in comparison with N-acetyl cysteine in axotomized rats

Iranian Journal of Basic Medical Sciences
مقاله 9، دوره 26، شماره 8، آبان 2023، صفحه 919-926    اصل مقاله (458.47 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.67157.14726
نویسندگان
Maryam Mousavinezhad-Moghaddam1؛ Morteza Behnam-Rassouli1؛ Narges Valizadeh2؛ Naser Mahdavi-Shahri1؛ Seyed Abdolrahim Rezaee* 2
1Biology Department, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): In this study, the impact of thiamine (Thi), N-acetyl cysteine (NAC), and dexamethasone (DEX) were investigated in axotomized rats, as a model for neural injury.
Materials and Methods: Sixty-five axotomized rats were divided into two different experimental approaches, the first experiments included five study groups (n=5): intrathecal Thi (Thi.it), intraperitoneal (Thi), NAC, DEX, and control. Cell survival was assessed in L5DRG in the 4th week by histological assessment. In the second study, 40 animals were engaged to assess Bcl-2, Bax, IL-6, and TNF-α expression in L4-L5DRG in the 1st and 2nd weeks after sural nerve axotomy under treatment of these agents (n=10).
Results: Ghost cells were observed in morphological assessment of L5DRG sections, and following stereological analysis, the volume and neuronal cell counts significantly were improved in the NAC and Thi.it groups in the 4th week (P<0.05). Although Bcl-2 expression did not show significant differences, Bax was reduced in the Thi group (P=0.01); and the Bcl-2/Bax ratio increased in the NAC group (1st week, P<0.01). Furthermore, the IL-6 and TNF-α expression decreased in the Thi and NAC groups, on the 1st week of treatment (P≤0.05 and P<0.01). However, in the 2nd week, the IL-6 expression in both Thi and NAC groups (P<0.01), and the TNF-α expression in the DEX group (P=0.05) were significantly decreased. 
Conclusion: The findings may classify Thi in the category of peripheral neuroprotective agents, in combination with routine medications. Furthermore, it had strong cell survival effects as it could interfere with the destructive effects of TNF-α by increasing Bax.
کلیدواژه‌ها
Axotomy؛ Inflammation؛ Neuro-protective؛ N-acetyl cysteine؛ Thiamine
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