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Shabestarian, Hoda, Homayouni Tabrizi, Masoud, Movahedi, Monireh, Neamati, Ali, Sharifnia, Fariba. (1402). Green synthesis of Ag-NPs as a metal nanoparticle and ZnO-NPs as a metal oxide nanoparticle: Evaluation of the in vitrocytotoxicity, anti-oxidant, anti-angiogenic activities. , 10(3), 245-258. doi: 10.22038/nmj.2023.71700.1770
Hoda Shabestarian; Masoud Homayouni Tabrizi; Monireh Movahedi; Ali Neamati; Fariba Sharifnia. "Green synthesis of Ag-NPs as a metal nanoparticle and ZnO-NPs as a metal oxide nanoparticle: Evaluation of the in vitrocytotoxicity, anti-oxidant, anti-angiogenic activities". , 10, 3, 1402, 245-258. doi: 10.22038/nmj.2023.71700.1770
Shabestarian, Hoda, Homayouni Tabrizi, Masoud, Movahedi, Monireh, Neamati, Ali, Sharifnia, Fariba. (1402). 'Green synthesis of Ag-NPs as a metal nanoparticle and ZnO-NPs as a metal oxide nanoparticle: Evaluation of the in vitrocytotoxicity, anti-oxidant, anti-angiogenic activities', , 10(3), pp. 245-258. doi: 10.22038/nmj.2023.71700.1770
Shabestarian, Hoda, Homayouni Tabrizi, Masoud, Movahedi, Monireh, Neamati, Ali, Sharifnia, Fariba. Green synthesis of Ag-NPs as a metal nanoparticle and ZnO-NPs as a metal oxide nanoparticle: Evaluation of the in vitrocytotoxicity, anti-oxidant, anti-angiogenic activities. , 1402; 10(3): 245-258. doi: 10.22038/nmj.2023.71700.1770

Green synthesis of Ag-NPs as a metal nanoparticle and ZnO-NPs as a metal oxide nanoparticle: Evaluation of the in vitrocytotoxicity, anti-oxidant, anti-angiogenic activities

Nanomedicine Journal
مقاله 9، دوره 10، شماره 3، مهر 2023، صفحه 245-258    اصل مقاله (724.72 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2023.71700.1770
نویسندگان
Hoda Shabestarian1؛ Masoud Homayouni Tabrizi* 2؛ Monireh Movahedi1؛ Ali Neamati2؛ Fariba Sharifnia3
1Department of Biochemistry, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
2Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3Department of Biology, Faculty of Biological Science, North Tehran Branch, Islamic Azad University, Tehran, Iran
چکیده
Objective(s): The study aimed to synthesize both silver- and zinc-oxide nanoparticles utilizing the Peganum harmala smoke extract (PHSE) bio-platform to evaluate their cytotoxicity on different types of human cancer cell lines and study their anti-oxidant, and anti-angiogenic potentials. 
Materials and Methods: The Silver (Ag) and zinc oxide (ZnO) nanoparticles (NP) were produced utilizing the green-synthesize method by applying the PHSE bio-platform. After characterization by X-Ray Diffraction (XRD), dynamic light scattering (DLS), Field emission scanning electron microscopy (FESEM), and Fourier-transform infrared spectroscopy (FTIR) methods. MTT assay was used for the evaluation toxicity of nanoparticles. ABTS, DPPH, FRAP, and ROS for anti-oxidant capacity, chicks’ chorioallantoic membrane (CAM), and qPCR for anti-angiogenesis effects of nanoparticles were used. 
Results: Ag-NPs (82.42 nm) and ZnO-NPs (163.05 nm) inhibited prostate, ovarian, and liver malignant cells. Inhibition of ABTS•+ and DPPH•+ and increasing the rate of intracellular ROS exhibited the anti and pro-oxidant capacity of Ag and ZnO-NPs out and inside of malignant cells. Also, their anti-angiogenesis impact was verified by significant dose-dependent VEGF and VEGFR down-regulation and the decreased blood vessels in the CAM. 
Conclusion: The anti-oxidant, cytotoxicity and anti-angiogenesis effects of Ag and ZnO-NPs synthesized from Pecan smoke extract make it possible to use these nanoparticles in cancer chemotherapy.
کلیدواژه‌ها
Angiogenesis inhibitors؛ Anti-oxidants؛ Metal nanoparticles
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