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Sun, Dongxia, Zhao, Yuanyuan, Wu, Xiaohua. (1402). Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions. , 26(8), 912-918. doi: 10.22038/ijbms.2023.68784.14993
Dongxia Sun; Yuanyuan Zhao; Xiaohua Wu. "Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions". , 26, 8, 1402, 912-918. doi: 10.22038/ijbms.2023.68784.14993
Sun, Dongxia, Zhao, Yuanyuan, Wu, Xiaohua. (1402). 'Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions', , 26(8), pp. 912-918. doi: 10.22038/ijbms.2023.68784.14993
Sun, Dongxia, Zhao, Yuanyuan, Wu, Xiaohua. Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions. , 1402; 26(8): 912-918. doi: 10.22038/ijbms.2023.68784.14993

Effects of tumor necrosis factor-α on glucose uptake in human granulosa cells under high androgen conditions

Iranian Journal of Basic Medical Sciences
مقاله 8، دوره 26، شماره 8، آبان 2023، صفحه 912-918    اصل مقاله (716.32 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68784.14993
نویسندگان
Dongxia Sun1، 2؛ Yuanyuan Zhao3؛ Xiaohua Wu* 1، 3
1Hebei Medical University, Shijiazhuang, 050011, China
2Prenatal Diagnosis Center, The Fourth Hospital of Shijiazhaung Affiliated to Hebei Medical University, Shijiazhuang 050011, China
3Institute of Reproductive Medicine of Shijiazhuang, The Fourth Hospital of Shijiazhuang, Gynecology and Obstetrics Hospital Affiliated to Hebei Medical University, Shijiazhuang 050011, China
چکیده
Objective(s): Hyperandrogenism is a key pathological characteristic of polycystic ovary syndrome (PCOS). Tumor necrosis factor α (TNF-α) is both an adipokine and a chronic inflammatory factor, which has been proven to be involved in the pathologic process of PCOS. This study aimed to determine how TNF-α affects glucose uptake in human granulosa cells in the presence of high testosterone concentration. 
Materials and Methods: KGN cell line was treated with testosterone and TNF-α alone or co-culture combination for 24 hr, or starved for 24 hr. Quantitative real-time polymerase chain reaction (qPCR) and western blot were performed to measure glucose transporter type 4 (GLUT4) message RNA (mRNA) and protein expression in treated KGN cells. Glucose uptake and GLUT4 expression were detected by immunofluorescence (IF). Furthermore, western blot was performed to measure the contents in the nuclear factor kappa-B (NF-κB) pathway. Meantime, upon addition of TNF-α receptor II (TNFRII) inhibitor or Inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) antagonist to block the TNFRII-IKKβ-NF-κB signaling pathway, the glucose uptake in KGN cells and GLUT4 translocation to cytomembrane were detected by IF, and related proteins in TNFRII-IKKβ-NF-κB were detected by western blot.
Results: The glucose uptake in Testosterone + TNF-α group was lowered significantly, and Total GLUT4 mRNA and proteins were reduced significantly. GLUT4 translocation to cytomembrane was tarnished visibly; concurrently, the phosphorylated proteins in the TNFRII-IKKβ-NF-κB signaling pathway were enhanced significantly. Furthermore, upon addition of TNFRII inhibitor or IKKβ inhibitor to block the TNFRII-IKKβ-NF-κB signaling pathway, the glucose uptake of treated granulosa cells was improved. 
Conclusion: TNFRII and IKKβ antagonists may improve glucose uptake in granulosa cells induced by TNF-α by blocking the TNFRII-IKKβ-NF-κB signaling pathway under high androgen conditions.
کلیدواژه‌ها
Glucose uptake؛ Granulosa cells؛ NF-kappa B signaling - pathway؛ Obesity؛ Polycystic ovary syndrome؛ TNF-α
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