سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Mohammadi, Mohsen, Moradi Hasan-Abad, Amin, Ghasemi, Ali. (1402). Evaluation of the antitumor activity of moronecidin (Piscidin)-like peptide in combination with anti-PD-1 antibody against melanoma tumor. , 26(9), 1061-1067. doi: 10.22038/ijbms.2023.69639.15166
Mohsen Mohammadi; Amin Moradi Hasan-Abad; Ali Ghasemi. "Evaluation of the antitumor activity of moronecidin (Piscidin)-like peptide in combination with anti-PD-1 antibody against melanoma tumor". , 26, 9, 1402, 1061-1067. doi: 10.22038/ijbms.2023.69639.15166
Mohammadi, Mohsen, Moradi Hasan-Abad, Amin, Ghasemi, Ali. (1402). 'Evaluation of the antitumor activity of moronecidin (Piscidin)-like peptide in combination with anti-PD-1 antibody against melanoma tumor', , 26(9), pp. 1061-1067. doi: 10.22038/ijbms.2023.69639.15166
Mohammadi, Mohsen, Moradi Hasan-Abad, Amin, Ghasemi, Ali. Evaluation of the antitumor activity of moronecidin (Piscidin)-like peptide in combination with anti-PD-1 antibody against melanoma tumor. , 1402; 26(9): 1061-1067. doi: 10.22038/ijbms.2023.69639.15166

Evaluation of the antitumor activity of moronecidin (Piscidin)-like peptide in combination with anti-PD-1 antibody against melanoma tumor

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 26، شماره 9، آذر 2023، صفحه 1061-1067    اصل مقاله (414.3 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.69639.15166
نویسندگان
Mohsen Mohammadi1؛ Amin Moradi Hasan-Abad2؛ Ali Ghasemi* 3، 4
1The Persian Gulf Marine Biotechnology Research Center, The Persian Gulf Biomedical Sciences Research Institute, Bushehr University of Medical Sciences, Bushehr, Iran
2Autoimmune Diseases Research Center, Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, Iran
3Department of Biochemistry and Hematology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan Iran
4Cancer Research Center, Semnan University of Medical Sciences, Semnan, Iran
چکیده
Objective(s): Immunotherapy has changed the landscape of oncology over the last decade and has become a standard of care for various cancers. Researchers previously demonstrated that B16-F10 melanoma in C57Bl6 mice is resistant to immune checkpoint inhibitors. The goal of this study was to investigate how anti-PD1 antibodies functioned in combination with a new antimicrobial peptide (AMP) called moronecidin-like peptide (MLP).
Materials and Methods: We studied the cytotoxic effect of AMP on the B10-F16 tumor cell line with the MTT experiment. The necrotic and apoptotic cells were determined by Presidium iodide (PI) /Annexin V staining and flow cytometry-based methods. Mice were inoculated subcutaneously with B10-F16 tumor cells in the mammary gland. Each group was sacrificed two weeks after the last injection to examine tumor-specific CD8+ T cell responses using flow cytometry. 
Results: Annexin V and PI staining assay revealed that MPL significantly induces apoptosis in B16F10 cells. It should be noted that MLP in combination with anti-PD-1 improved antigen-specific T-cell responses synergistically (P=0.01) when compared with respective monotherapy. Furthermore, when compared with the respective monotherapies, combination therapy significantly controlled tumor growth in B10-F16 tumor cells and increased survival rate.
Conclusion: Treatments with anti-PD-1 inhibitors alone had only a minor effect on tumor size, whereas combination therapy resulted in significant tumor growth control and increased animal survival. MLP therapy combined with anti-PD-1 antibody improves anti-tumor immune response in addition to inducing tumor cell apoptosis. As a result, the evidence suggests that intratumoral injection of MPL can improve anti-PD-1 antibody antitumor response.
کلیدواژه‌ها
Anti-PD-1؛ Antibody؛ Antimicrobial peptides؛ Cancer therapy؛ Immunotherapy؛ Melanoma cancer
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