سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Yaghoubi, Atieh, Movaqar, Aref, Asgharzadeh, Fereshteh, Derakhshan, Mohammad, Ghazvini, Kiarash, Hasanian, Seyed Mahdi, Avan, Amir, Mostafapour, Asma, Khazaei, Majid, Soleimanpour, Saman. (1402). Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy. , 26(7), 768-776. doi: 10.22038/ijbms.2023.68331.14913
Atieh Yaghoubi; Aref Movaqar; Fereshteh Asgharzadeh; Mohammad Derakhshan; Kiarash Ghazvini; Seyed Mahdi Hasanian; Amir Avan; Asma Mostafapour; Majid Khazaei; Saman Soleimanpour. "Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy". , 26, 7, 1402, 768-776. doi: 10.22038/ijbms.2023.68331.14913
Yaghoubi, Atieh, Movaqar, Aref, Asgharzadeh, Fereshteh, Derakhshan, Mohammad, Ghazvini, Kiarash, Hasanian, Seyed Mahdi, Avan, Amir, Mostafapour, Asma, Khazaei, Majid, Soleimanpour, Saman. (1402). 'Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy', , 26(7), pp. 768-776. doi: 10.22038/ijbms.2023.68331.14913
Yaghoubi, Atieh, Movaqar, Aref, Asgharzadeh, Fereshteh, Derakhshan, Mohammad, Ghazvini, Kiarash, Hasanian, Seyed Mahdi, Avan, Amir, Mostafapour, Asma, Khazaei, Majid, Soleimanpour, Saman. Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy. , 1402; 26(7): 768-776. doi: 10.22038/ijbms.2023.68331.14913

Anticancer activity of Pseudomonas aeruginosa derived peptide with iRGD in colon cancer therapy

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 26، شماره 7، مهر 2023، صفحه 768-776    اصل مقاله (1.62 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68331.14913
نویسندگان
Atieh Yaghoubi1، 2؛ Aref Movaqar1؛ Fereshteh Asgharzadeh2، 3؛ Mohammad Derakhshan1؛ Kiarash Ghazvini1؛ Seyed Mahdi Hasanian4؛ Amir Avan5؛ Asma Mostafapour6؛ Majid Khazaei* 3؛ Saman Soleimanpour* 1
1Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
2Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical, Sciences, Mashhad, Iran
5Department of Medical Genetics and Molecular Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
چکیده
Objective(s): Colon cancer is well-known as a life-threatening disease. Since the current treatment modalities for this type of cancer are powerful yet face some limitations, finding novel treatments is required to achieve better outcomes with fewer side effects. Here we investigated the therapeutic potential of Azurin-p28 alone or along with iRGD (Ac-CRGDKGPDC-amide) as a tumor-penetrating peptide and 5-fluorouracil (5-FU) for colon cancer. 
Materials and Methods: Inhibitory effect of p28 with or without iRGD/5-FU was studied in CT26 and HT29, as well as the xenograft animal model of cancer. The effect of p28 alone or along with iRGD/5-FU on cell migration, apoptotic activity, and cell cycle of the cell lines was assessed. Level of the BAX and BCL2 genes, tumor suppressor genes [(p53 and collagen type-Iα1 (COL1A1), collagen type-Iα2 (COL1A2)] were assessed by quantitative RT-PCR.
Results: These findings show that using p28 with or without iRGD and 5-FU raised the level of p53 and BAX but decreased BCL2, compared with control and 5-FU groups in tissues of the tumor, which result in raising the apoptosis. 
Conclusion: It seems that p28 may be used as a new therapeutic approach in colon cancer therapy that can enhance the anti-tumor effect of 5-FU.
کلیدواژه‌ها
Azurin-p28؛ Bacterial peptide؛ Colon cancer؛ iRGD؛ Pseudomonas aeruginosa
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