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Zhang, Degang, Shen, Fengqin, Ma, Shiting, Nan, Suting, Ma, Yanhong, Ren, Lili, Li, Hao, Yu, Qin. (1402). Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway. , 26(6), 653-661. doi: 10.22038/ijbms.2023.68827.15000
Degang Zhang; Fengqin Shen; Shiting Ma; Suting Nan; Yanhong Ma; Lili Ren; Hao Li; Qin Yu. "Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway". , 26, 6, 1402, 653-661. doi: 10.22038/ijbms.2023.68827.15000
Zhang, Degang, Shen, Fengqin, Ma, Shiting, Nan, Suting, Ma, Yanhong, Ren, Lili, Li, Hao, Yu, Qin. (1402). 'Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway', , 26(6), pp. 653-661. doi: 10.22038/ijbms.2023.68827.15000
Zhang, Degang, Shen, Fengqin, Ma, Shiting, Nan, Suting, Ma, Yanhong, Ren, Lili, Li, Hao, Yu, Qin. Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway. , 1402; 26(6): 653-661. doi: 10.22038/ijbms.2023.68827.15000

Andrographolide alleviates paraquat-induced acute lung injury by activating the Nrf2/HO-1 pathway

Iranian Journal of Basic Medical Sciences
مقاله 6، دوره 26، شماره 6، شهریور 2023، صفحه 653-661    اصل مقاله (469.79 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.68827.15000
نویسندگان
Degang Zhang1، 2؛ Fengqin Shen2؛ Shiting Ma2؛ Suting Nan2؛ Yanhong Ma2؛ Lili Ren2؛ Hao Li2؛ Qin Yu* 1، 2، 3
1Lanzhou University, Lanzhou, Gansu Province, 730000, P.R. China
2Health Science Center, Lanzhou University, Lanzhou, Gansu Province, 730000, P.R. China
3Respiratory and Critical Care Medicine, The first hospital of Lanzhou University, Lanzhou, Gansu Province, 730000, P.R. China
چکیده
Objective(s): Paraquat (PQ), a highly effective and rapidly non-selective herbicide, mainly targets the lungs and causes acute lung injury (ALI). So far, the scarcity of effective drug candidates against PQ-induced ALI remains a big challenge. Andrographolide (Andro), with its anti-inflammatory and antioxidant activities, has been demonstrated to alleviate ALI. Nevertheless, whether Andro could alleviate the PQ-mediated ALI remains unknown. Therefore, this study will explore the effects as well as the possible mechanism of Andro against ALI caused by PQ. 
Materials and Methods: C57BL/6J mice were injected with 20 mg/kg PQ intraperitoneally to establish an ALI model. PQ-treated MLE-12 cells were applied to a vitro model. Nuclear factor erythroid like-2 (Nrf2) was knocked out to explore the specific effects of the Nrf2/ Heme oxygenase-1 (OH-1) pathway in the protection of Andro against ALI caused by PQ.
Results: Andro significantly reduced lung damage and the ratio of Wet/Dry (W/D) weight, decreased MDA, IL-6, IL-1β, and TNF-ɑ levels, reversed the decrease of CAT and SOD levels, and inhibited apoptosis caused by PQ. Andro obviously increased the ratio of Bcl-2/Bax while reducing caspase-3 and cleaved caspase-3 levels. Furthermore, Andro dramatically elevated the antioxidant proteins Nrf2, NQO-1, and HO-1 levels compared with the PQ group. This experiment demonstrated that Andro reduced ROS and inhibited apoptosis, induced by PQ in MLE-12 cells, by inducing Nrf2/HO-1 pathway activation.
Conclusion: Andro effectively ameliorates oxidant stress and apoptosis in ALI caused by PQ, possibly through inducing Nrf2/HO-1 pathway activation.
کلیدواژه‌ها
Acute lung injury؛ Andrographolide؛ Apoptosis؛ Nrf2؛ Oxidative stress؛ Paraquat
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