Preparation and Characterization of Platelet-Derived Exosomes, as a Nanostructure for bio-compound delivery | ||
| Nanomedicine Journal | ||
| مقاله 5، دوره 10، شماره 2، تیر 2023، صفحه 131-137 اصل مقاله (466.06 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22038/nmj.2023.70974.1758 | ||
| نویسندگان | ||
| seyyede Fatemeh Shams؛ Mahshid Mohammadipour* ؛ Mohammadreza Deyhim | ||
| Blood Transfusion Research Center, High Institute for Research and Education in transfusion Medicine, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Exosomes are extracellular vesicles (EV), which are released from the endosomal membrane of the cells. They are 30-120 nm in size, and carry many biological substances such as protein, lipids, and RNA. All kinds of cells release exosomes during their life. Exosomes are a small showcase of the mother cell’s contents. Platelets-derived exosomes (Exo-Plt) are the most frequent nanoscale particles in the peripheral blood. Their availability and non-toxicity as an autologous carrier and a growth factor cargo made us introduce the most efficient method to isolate exosomes from platelet products and also examine their ability as a delivery system. Materials and Methods: Two different protocols including stimulated and unstimulated were used in the present study to obtain Exo-Plt. Dynamic light scattering (DLS), BCA assay, Transmission Electron microscopy (TEM), and western blotting were performed to assess the basic characteristics (size and zeta potential, protein content, morphology, and CD markers, respectively) of the isolated particles. Consequently, the ability of Exo-plts for delivery of exogenous miRNA into the model cell line was assessed by Real-Time PCR. Results: The stimulated method was more efficient to isolate Exo-Plt. Additionally, the applied agonist and the time of sample preparation from individuals affected the exosome yield. The Exo-Plts prosperously delivered the exogenous cargo into the cell line. Conclusion: This research provided a standard protocol to isolate high-quality and pure Exo-Plt for bio-compound delivery and even therapeutic objectives. Exosomes provide the opportunity for cell-free therapy. | ||
| کلیدواژهها | ||
| Drug delivery systems؛ Extra cellular vesicles؛ Platelet rich plasma؛ Transmission electron microscopy | ||
| مراجع | ||
|
1. Barclay RA, Pleet ML, Akpamagbo Y, Noor K, Mathiesen A, Kashanchi F. Isolation of exosomes from HTLV-infected cells. Human T-Lymphotropic Viruses: Methods Protoc. 2017:57-75. 2. Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, et al. Reassessment of exosome composition. Cell. 2019;177(2):428-445. 3. Javan MR, Kafi-Abad SA, Zarif MN, Balagholi S, Dabbaghi R, Karami S. In-line leukoreduction filters: A new source of microparticle for human and animal study. Transfus Apher Sci. 2022:103602. 4. Wu J, Piao Y, Liu Q, Yang X. Platelet‐rich plasma‐derived extracellular vesicles: A superior alternative in regenerative medicine? Cell Prolif. 2021;54(12):e13123. 5. Heidarzadeh M, Gürsoy-Özdemir Y, Kaya M, Eslami Abriz A, Zarebkohan A, Rahbarghazi R, et al. Exosomal delivery of therapeutic modulators through the blood–brain barrier; promise and pitfalls. Cell Biosci. 2021;11(1):1-28. 6. Munagala R, Aqil F, Jeyabalan J, Kandimalla R, Wallen M, Tyagi N, et al. Exosome-mediated delivery of RNA and DNA for gene therapy. Cancer Lett. 2021;505:58-72. 7. Hunter MP, Ismail N, Zhang X, Aguda BD, Lee EJ, Yu L, et al. Detection of microRNA expression in human peripheral blood microvesicles. PLoS One. 2008;3(11):e3694. 8. Everts P, Onishi K, Jayaram P, Lana JF, Mautner K. Platelet-rich plasma: new performance understandings and therapeutic considerations in 2020. Int J Mol Sci 2020;21(20):7794. 9. Johnson J, Wu Y-W, Blyth C, Lichtfuss G, Goubran H, Burnouf T. Prospective therapeutic applications of platelet extracellular vesicles. Trends Biotechnol. 2021;39(6):598-612. 10. Iyer SR, Scheiber AL, Yarowsky P, Henn III RF, Otsuru S, Lovering RM. Exosomes isolated from platelet-rich plasma and mesenchymal stem cells promote recovery of function after muscle injury. Am J Sports Med. 2020;48(9):2277-2286. 11. Liu X, Wang L, Ma C, Wang G, Zhang Y, Sun S. Exosomes derived from platelet-rich plasma present a novel potential in alleviating knee osteoarthritis by promoting proliferation and inhibiting apoptosis of chondrocyte via Wnt/β-catenin signaling pathway. J Orthop Surg Res. 2019;14(1):1-16. 12. Moen M, Weir A, Bakker E, Rekers M, Laudy G. Efficacy of platelet-rich plasma injections in osteoarthritis of the knee: an updated systematic review and meta-analysis. Osteoarthritis Cartilage. 2016;24: 520-21. 13. Nilforoushzadeh MA, Aghdami N, Taghiabadi E. Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells. Cell J (Yakhteh). 2021;23(5):576-583. 14. Aatonen MT, Öhman T, Nyman TA, Laitinen S, Grönholm M, Siljander PR-M. Isolation and characterization of platelet-derived extracellular vesicles. J Extracell Vesicles. 2014;3(1):24692. 15. Zhang D, Lee H, Zhu Z, Minhas JK, Jin Y. Enrichment of selective miRNAs in exosomes and delivery of exosomal miRNAs in vitro and in vivo. Am J Physiol Lung Cell Mol Physiol. 2017;312(1): 110-21. 16. Rio DC, Ares M, Hannon GJ, Nilsen TW. Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010;5(6): 5439. 17. Tominaga N, Hagiwara K, Kosaka N, Honma K, Nakagama H, Ochiya T. RPN2-mediated glycosylation of tetraspanin CD63 regulates breast cancer cell malignancy. Mol Cancer. 2014;13(1):1-11. 18. Ambrose AR, Alsahli MA, Kurmani SA, Goodall AH. Comparison of the release of microRNAs and extracellular vesicles from platelets in response to different agonists. Platelets. 2018;29(5):446-454. 19. Saumell-Esnaola M, Delgado D, García del Caño G, Beitia M, Sallés J, González-Burguera I, et al. Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization. Int J Mol Sci. International Journal of Molecular Sciences. 2022;23(5):2861. 20. Staindl O. The healing of wounds and scar formation under the influence of a tissue adhesion system with fibrinogen, thrombin, and coagulation factor XIII. Arch Otorhinolaryngol. 1979;222(4):241-245. 21. Azevedo LC JM, Pontieri V, Pedro MD, Bassi E, Tucci PJ, Laurindo FR. Platelet-derived exosomes from septic shock patients induce myocardial dysfunction. Crit Care. 2007;11(6):1-10. 22. Dash M, Palaniyandi K, Ramalingam S, Sahabudeen S, Raja N. Exosomes isolated from two different cell lines using three different isolation techniques show variation in physical and molecular characteristics. Biochim Biophys Acta Biomembr. 2021;1863(2):183490. 23. Bagheri S, Samiee S, Zarif MN, Deyhim MR. l-carnitine modulates free mitochondrial DNA DAMPs and platelet storage lesions during storage of platelet concentrates. J Thromb Thrombolysis. 2022;55(1):60-66. 24. Collier ME, Ambrose AR, Goodall AH. Does hsa-miR-223-3p from platelet-derived extracellular vesicles regulate tissue factor expression in monocytic cells? Platelets. 2022;33(7):1031-1042. 25. Zhang Y, Bi J, Huang J, Tang Y, Du S, Li P. Exosome: a review of its classification, isolation techniques, storage, diagnostic and targeted therapy applications. Int J Nanomedicine. 2020;15:6917-6934. | ||
|
آمار تعداد مشاهده مقاله: 570 تعداد دریافت فایل اصل مقاله: 732 |
||