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Deng, Xiaoyu, He, Jinke, Xu, Jinfeng, Wang, Yueli, Yi, Jihai, Zhang, Huan, Wang, Yong, Wang, Zhen, Chen, Chuangfu. (1402). LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice. , 26(5), 564-571. doi: 10.22038/ijbms.2023.67394.14775
Xiaoyu Deng; Jinke He; Jinfeng Xu; Yueli Wang; Jihai Yi; Huan Zhang; Yong Wang; Zhen Wang; Chuangfu Chen. "LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice". , 26, 5, 1402, 564-571. doi: 10.22038/ijbms.2023.67394.14775
Deng, Xiaoyu, He, Jinke, Xu, Jinfeng, Wang, Yueli, Yi, Jihai, Zhang, Huan, Wang, Yong, Wang, Zhen, Chen, Chuangfu. (1402). 'LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice', , 26(5), pp. 564-571. doi: 10.22038/ijbms.2023.67394.14775
Deng, Xiaoyu, He, Jinke, Xu, Jinfeng, Wang, Yueli, Yi, Jihai, Zhang, Huan, Wang, Yong, Wang, Zhen, Chen, Chuangfu. LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice. , 1402; 26(5): 564-571. doi: 10.22038/ijbms.2023.67394.14775

LDH as an adjuvant makes Brucella outer-membrane vesicles and outer-membrane vesicle-associated proteins highly protective in mice

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 26، شماره 5، مرداد 2023، صفحه 564-571    اصل مقاله (1.46 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2023.67394.14775
نویسندگان
Xiaoyu Deng1، 2؛ Jinke He3؛ Jinfeng Xu2، 4؛ Yueli Wang1، 2؛ Jihai Yi1، 2؛ Huan Zhang1، 2؛ Yong Wang1، 2؛ Zhen Wang* 1، 2؛ Chuangfu Chen* 1، 2
1School of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang 832003, China
2Collaborative Innovation Center for Prevention and Control of High Incidence Zoonotic Infectious Diseases in Western China, College of Animal Science and Technology, Shihezi University, Shihezi, China
3Department of Basic Medicine, Xinjiang Second Medical College, Kelamayi, Xinjiang, China
4Wuwei vocational college, Wuwei, Gansu 733000, China
چکیده
Objective(s): Existing Brucella vaccines are attenuated and can cause vaccine-associated brucellosis; and these safety concerns have affected their application. Although subunit vaccines have the advantages of safety, efficacy, low cost, and rapid production, they are usually poorly immunogenic and insufficient to trigger persistent immunity. Therefore, we added layered double hydroxide (LDH) as an adjuvant to Brucella subunit vaccine formulations to enhance the immune response to the antigen.
Materials and Methods: LDH and Freund’s adjuvant were combined with Brucella outer-membrane vesicles (OMVs) and OMV-associated proteins to form a subunit vaccine, respectively. The immunogenicity of LDH as an adjuvant was assessed in BALB/c mice. We examined levels of immunoglobulin G, G1, and G2a (IgG, IgG1, and IgG2a) antibodies (aBs); percentages of Cluster of Differentiation 4-positive (CD4+) and CD8+ T cells in peripheral-blood lymphocytes; and secretion of cytokines in mouse spleen lymphocytes. Finally, splenic index and splenic bacterial load were assessed via Brucella challenge experiments on mice.
Results: The LDH subunit vaccine also produced high levels of specific aBs in mice compared with Freund’s adjuvant subunit vaccine and induced mainly T-helper 1 cell (Th1)-type immune responses. In addition, mice in the LDH subunit vaccine group had significantly lower bacterial loads in their spleens than those in the Freund’s adjuvant subunit vaccine group, and the LDH-OMV vaccine offered a higher level of protection against Brucella attack.
Conclusion: LDH as an adjuvant-paired vaccine provided a high level of protection against Brucella infection.
کلیدواژه‌ها
Adjuvant؛ Brucella؛ Immunogenicity؛ Outer-membrane vesicles Vaccine
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