سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Bahrami-Banan, Fatemeh, Sheikhha, Mohammad Hasan, Ghasemi, Nasrin, Rahmani, Masoumeh, Hemati, Mahdie, Safdari, Mohammadreza, Haghiralsadat, Fatemeh. (1401). Synthesis of a stimuli-sensitive PEGylated nanoniosomal doxorubicin for the treatment of acute myeloid leukemia: An in vitro study. , 10(1), 77-84. doi: 10.22038/nmj.2022.69777.1745
Fatemeh Bahrami-Banan; Mohammad Hasan Sheikhha; Nasrin Ghasemi; Masoumeh Rahmani; Mahdie Hemati; Mohammadreza Safdari; Fatemeh Haghiralsadat. "Synthesis of a stimuli-sensitive PEGylated nanoniosomal doxorubicin for the treatment of acute myeloid leukemia: An in vitro study". , 10, 1, 1401, 77-84. doi: 10.22038/nmj.2022.69777.1745
Bahrami-Banan, Fatemeh, Sheikhha, Mohammad Hasan, Ghasemi, Nasrin, Rahmani, Masoumeh, Hemati, Mahdie, Safdari, Mohammadreza, Haghiralsadat, Fatemeh. (1401). 'Synthesis of a stimuli-sensitive PEGylated nanoniosomal doxorubicin for the treatment of acute myeloid leukemia: An in vitro study', , 10(1), pp. 77-84. doi: 10.22038/nmj.2022.69777.1745
Bahrami-Banan, Fatemeh, Sheikhha, Mohammad Hasan, Ghasemi, Nasrin, Rahmani, Masoumeh, Hemati, Mahdie, Safdari, Mohammadreza, Haghiralsadat, Fatemeh. Synthesis of a stimuli-sensitive PEGylated nanoniosomal doxorubicin for the treatment of acute myeloid leukemia: An in vitro study. , 1401; 10(1): 77-84. doi: 10.22038/nmj.2022.69777.1745

Synthesis of a stimuli-sensitive PEGylated nanoniosomal doxorubicin for the treatment of acute myeloid leukemia: An in vitro study

Nanomedicine Journal
مقاله 8، دوره 10، شماره 1، فروردین 2023، صفحه 77-84    اصل مقاله (805.64 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22038/nmj.2022.69777.1745
نویسندگان
Fatemeh Bahrami-Banan1؛ Mohammad Hasan Sheikhha1؛ Nasrin Ghasemi1؛ Masoumeh Rahmani2؛ Mahdie Hemati2؛ Mohammadreza Safdari* 3، 4؛ Fatemeh Haghiralsadat* 2
1Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Science, Yazd, Iran
2Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3Orthopedic Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Orthopedic Surgery, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
چکیده
Objective(s): One of the effective strategies for targeted chemotherapy of cancer is the use of lipid nanocarriers. In this study, an optimal formulation of niosomal drug containing doxorubicin was developed to monitor the potency against cancer cells. 
Materials and Methods: In this experimental study, niosomal vesicles were prepared using phosphatidylcholine (20%), span60 (52.5%), cholesterol (22.5%), and DSPE-PEG2000 (5%) by the thin-film method. Doxorubicin was loaded into the niosomes using an inactive loading method. 
Results: The features and characteristics of the nanocarrier were evaluated using Zeta-Sizer, SEM, FTIR, drug release, cellular uptake, and the cytotoxicity of the nanodrug carrier system by the MTT method. Niosomal vesicles-containing doxorubicin showed a size of ~156.8 nm, drug encapsulation efficiency of ~94.18%, zeta potential of ~-3.52 mV, and polydispersity index (PDI) of ~0.265. The prepared niosomes indicated a drug-controlled release system and FTIR analysis showed no interaction between nanocarriers containing drug and doxorubicin. Moreover, morphological examination of nanocarriers using SEM microscopy revealed that they had spherical structures. Also, cellular studies showed that drug toxicity was higher in encapsulated form of the drug compared with non-encapsulated doxorubicin which was confirmed by the cellular uptake results. 
Conclusion: The results confirmed the proper physicochemical characteristics of these nanocarriers that significantly increased the toxicity of the encapsulated drug against the KG-1 cell line. It seems niosomal nanocarriers can be considered suitable carriers for drug delivery to cancer cells.
کلیدواژه‌ها
Acute myeloid leukemia؛ Doxorubicin؛ KG1 cell line؛ Niosomes
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