Pirali Hamedani, Mostafa, Ahmad Kashi, Mahtab, Goodarzi, Saied, Shokouhi, Hadiseh, Shafiee Ardestani, Mehdi, Hadjiakhoondi, Abbas, Pirali Hamedani, Morteza, Ghahremani, Mohammad Hosein, Mehrbod, Parvaneh, Tofighi, Zahra. (1401). Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus. , 26(1), 76-84. doi: 10.22038/ijbms.2022.66255.14553
Mostafa Pirali Hamedani; Mahtab Ahmad Kashi; Saied Goodarzi; Hadiseh Shokouhi; Mehdi Shafiee Ardestani; Abbas Hadjiakhoondi; Morteza Pirali Hamedani; Mohammad Hosein Ghahremani; Parvaneh Mehrbod; Zahra Tofighi. "Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus". , 26, 1, 1401, 76-84. doi: 10.22038/ijbms.2022.66255.14553
Pirali Hamedani, Mostafa, Ahmad Kashi, Mahtab, Goodarzi, Saied, Shokouhi, Hadiseh, Shafiee Ardestani, Mehdi, Hadjiakhoondi, Abbas, Pirali Hamedani, Morteza, Ghahremani, Mohammad Hosein, Mehrbod, Parvaneh, Tofighi, Zahra. (1401). 'Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus', , 26(1), pp. 76-84. doi: 10.22038/ijbms.2022.66255.14553
Pirali Hamedani, Mostafa, Ahmad Kashi, Mahtab, Goodarzi, Saied, Shokouhi, Hadiseh, Shafiee Ardestani, Mehdi, Hadjiakhoondi, Abbas, Pirali Hamedani, Morteza, Ghahremani, Mohammad Hosein, Mehrbod, Parvaneh, Tofighi, Zahra. Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus. , 1401; 26(1): 76-84. doi: 10.22038/ijbms.2022.66255.14553

Elucidation of flavonoids from potent Iranian Scutellaria species against Influenza A (H1N1) virus

Iranian Journal of Basic Medical Sciences
مقاله 10، دوره 26، شماره 1، فروردین 2023، صفحه 76-84    اصل مقاله (763.18 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.66255.14553
نویسندگان
Mostafa Pirali Hamedani1؛ Mahtab Ahmad Kashi1؛ Saied Goodarzi2؛ Hadiseh Shokouhi3؛ Mehdi Shafiee Ardestani4؛ Abbas Hadjiakhoondi1، 2؛ Morteza Pirali Hamedani5؛ Mohammad Hosein Ghahremani6؛ Parvaneh Mehrbod* 3؛ Zahra Tofighi* 1، 2
1Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3Influenza and Respiratory Viruses Department, Pasteur Institute of Iran, Tehran, Iran
4Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
5Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
6Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
چکیده
Objective(s): Influenza A virus (IAV) is a contagious illness. Different species of Scutellaria genus are used as a traditional remedy to reduce influenza symptoms. This study aimed to investigate the anti-influenza capacity of several species of Iranian Scutellaria and identify active compounds of the most potent species for the first time.
Materials and Methods: Some Iranian species of Scutellaria were collected from different regions of Iran, including S. pinnatifida with mucida, viridis, and alpina subspecies; S. tournefortii; S. tomentosa; S. persica. They were fractionated to chloroform and methanol. The total phenols and flavonoids of samples were examined by the folin-ciocalteau and aluminum-flavonoid complex methods, respectively. The 50% cytotoxic concentrations (CC50) on MDCK cells and non-cytotoxic concentrations (NCTC) were determined by MTT assay. The percentage of cell protection against IAV and their effect on virus titer were investigated in pre-, post-, and co-penetration treatment groups. Phytochemicals of the most effective species were isolated by various chromatographic methods and identified by different spectroscopic methods.
Results: Methanol fraction of S. pinnatifida subsp. viridis demonstrated the highest amounts of flavonoid content and best activity against influenza A virus in all combination treatments, which reduced the virus titer by 5 logs with no cytotoxicity. Kaempferol-3-O-glucoside, quercetin-3-O-glucoside, apigenin-4′-methoxy-7-O-glucoside, luteolin, and luteolin-7-O-glucoside were purified and identified from this species.
Conclusion: Scutellaria pinnatifida subsp. viridis can be introduced as a source of flavonoids with acceptable anti-influenza activity. S. tomentosa also showed potent antiviral effects and is a candidate for elucidation in further studies.
کلیدواژه‌ها
Antiviral؛ Flavonoids؛ Folin-ciocalteau reagent؛ H1N1 virus؛ MTT assay؛ Scutellaria
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