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Li, Deming, Guo, Xiaoshu, Zhao, Wenyu, Chen, Jingyu, Xia, Cong, Yu, Guoying. (1401). Genome-wide DNA methylation dynamics in carbon tetrachloride-induced mice liver fibrosis. , 26(1), 85-92. doi: 10.22038/ijbms.2022.66256.14555
Deming Li; Xiaoshu Guo; Wenyu Zhao; Jingyu Chen; Cong Xia; Guoying Yu. "Genome-wide DNA methylation dynamics in carbon tetrachloride-induced mice liver fibrosis". , 26, 1, 1401, 85-92. doi: 10.22038/ijbms.2022.66256.14555
Li, Deming, Guo, Xiaoshu, Zhao, Wenyu, Chen, Jingyu, Xia, Cong, Yu, Guoying. (1401). 'Genome-wide DNA methylation dynamics in carbon tetrachloride-induced mice liver fibrosis', , 26(1), pp. 85-92. doi: 10.22038/ijbms.2022.66256.14555
Li, Deming, Guo, Xiaoshu, Zhao, Wenyu, Chen, Jingyu, Xia, Cong, Yu, Guoying. Genome-wide DNA methylation dynamics in carbon tetrachloride-induced mice liver fibrosis. , 1401; 26(1): 85-92. doi: 10.22038/ijbms.2022.66256.14555

Genome-wide DNA methylation dynamics in carbon tetrachloride-induced mice liver fibrosis

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 26، شماره 1، فروردین 2023، صفحه 85-92    اصل مقاله (1.51 M)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.66256.14555
نویسندگان
Deming Li1؛ Xiaoshu Guo1، 2؛ Wenyu Zhao1؛ Jingyu Chen1؛ Cong Xia1؛ Guoying Yu* 1
1State Key Laboratory Cell Differentiation and Regulation, Henan International Joint Laboratory of Pulmonary Fibrosis, Henan Center for Outstanding Overseas Scientists of Pulmonary Fibrosis, Overseas Expertise Introduction Center for Discipline Innovation of Pulmonary Fibrosis (111 Project), College of Life Science, Henan Normal University, Xinxiang, Henan, China
2Department of Physiology, Changzhi Medical College, Shanxi, China 2.Department of Physiology, Changzhi Medical College
چکیده
Objective(s): Many persistent harmful stimuli can result in chronic liver diseases, which lead to about 2 million deaths per year in the whole world. Liver fibrosis was found to exist in all kinds of chronic liver diseases. Many studies suggested that DNA methylation was associated with the pathogenesis of liver fibrosis. This study aimed to quantitatively detect DNA methylation changes in the whole genome in fibrotic liver tissues of mice.
Materials and Methods: Liver fibrosis was induced by intraperitoneal injection of carbon tetrachloride (CCl4) for 4 weeks. A genome-wide methylome analysis was performed using 850K BeadChips assays. The methylation status of 27 CpG dinucleotides located in 3 genes was detected by pyrosequencing to confirm chip data accuracy, and mRNA expressions of these 3 genes were examined by RT-qPCR methods.
Results: A total of 130,068 differentially methylated sites (DMS, 58,474 hypermethylated, and 71,594 hypomethylated) between fibrotic liver tissues and control mice liver tissues were identified by the 850k BeadChips array. Consistency between pyrosequencing data and 850k BeadChips array data was observed (R=0.928; P<0.01). Apoptosis, positive regulation of transcription of Notch receptor target, and negative regulation of p38MAPK signal cascade activities were significantly enriched in the Gene Ontology (GO) analyses. Cholesterol metabolism, bile secretion, and more biosynthesis and metabolism pathways were enriched in KEGG pathway analyses. Ten key genes were identified by the Cytoscape plugin cytoHubba. 
Conclusion: 7850 genes were found to have methylation change in fibrotic liver tissues of mice, which facilitates future research for clinical application.
کلیدواژه‌ها
CCl4؛ DNA؛ Fibrosis؛ Liver؛ Methylation
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