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Cao, Yiqiu, Dong, Zhu, Yang, Dongpeng, Wang, Xiaowu. (1401). LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation. , 26(1), 30-36. doi: 10.22038/ijbms.2022.65006.14313
Yiqiu Cao; Zhu Dong; Dongpeng Yang; Xiaowu Wang. "LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation". , 26, 1, 1401, 30-36. doi: 10.22038/ijbms.2022.65006.14313
Cao, Yiqiu, Dong, Zhu, Yang, Dongpeng, Wang, Xiaowu. (1401). 'LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation', , 26(1), pp. 30-36. doi: 10.22038/ijbms.2022.65006.14313
Cao, Yiqiu, Dong, Zhu, Yang, Dongpeng, Wang, Xiaowu. LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation. , 1401; 26(1): 30-36. doi: 10.22038/ijbms.2022.65006.14313

LSD1 in beige adipocytes protects cardiomyocytes against oxygen and glucose deprivation

Iranian Journal of Basic Medical Sciences
مقاله 5، دوره 26، شماره 1، فروردین 2023، صفحه 30-36    اصل مقاله (620.19 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65006.14313
نویسندگان
Yiqiu Cao1، 2؛ Zhu Dong3، 4؛ Dongpeng Yang3، 4، 5؛ Xiaowu Wang* 3، 4
1Department of Cardiac Surgery, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, 570311, People’s Republic of China
2The First School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People’s Republic of China
3Department of Cardiovascular Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, People’s Republic of China
42 The First School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, People’s Republic of China
5Department of Cardiovascular Surgery, Guangzhou Red Cross Hospital, Jinan University, 510235, People’s Republic of China
چکیده
Objective(s): Epicardial adipose tissue (EpAT) is known for its role in supporting the cardiomyocytes. Lysine-specific demethylase 1 (LSD1), a typical lysine demethylase, is an essential regulator for the maintenance of beige adipocytes. However, the effect of LSD1 in the adipogenic differentiation of beige adipocytes in EpAT, and its function on oxygen and glucose deprivation (OGD)-injured cardiomyocytes remain unclear.
Materials and Methods: Heart tissues from young mice and elder mice were collected for immunohistochemical staining. LSD1 in 3T3-L1 cells was knocked down by LSD1-shRNA lentivirus infection. The qRT-PCR, western blotting, and Oil Red O staining were employed to detect the adipogenic differentiation of 3T3-L1 cells and formation of beige adipocytes. The cardiomyocytes co-cultured with beige adipocytes were used for OGD treatment. Cell apoptosis was analyzed by flow cytometry. The lactate dehydrogenase (LDH) and superoxide dismutase (SOD) activity were analyzed using commercially available kits.
Results: The decrease of LSD1 was related to the age-dependent loss of beige adipocytes in mice EpAT. LSD1 knockdown inhibited the adipogenic differentiation of 3T3-L1 cells and formation of beige adipocytes. The down-regulation of LSD1 in 3T3-L1 cells decreased the protective effect of mature adipocytes on OGD-injured cardiomyocytes.
Conclusion: The decreased expression of LSD1 in mice EpAT was associated with age-dependent ablation of beige adipocytes. The protective effect of beige adipocytes on OGD-injured cardiomyocytes is reduced by knockdown of LSD1 in adipocytes. The present study provided exciting insights into establishing novel therapies against age-dependent cardiac diseases.
کلیدواژه‌ها
Beige adipocyte؛ Cardiomyocyte؛ Epicardial adipose tissue؛ Lysine-specific demethylase 1؛ Oxygen and glucose-deprivation
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