Albiflorin alleviates DSS-induced ulcerative colitis in mice by reducing inflammation and oxidative stress | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 7، دوره 26، شماره 1، فروردین 2023، صفحه 48-56 اصل مقاله (1.28 M) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2022.66678.14624 | ||
| نویسندگان | ||
| Xiaohui Wang1؛ Lianlin Su2؛ Jinhua Tan1؛ Tianwen Ding3؛ Yinzi Yue* 3 | ||
| 1Department of General Surgery, Bayinguoleng Mongolian Autonomous Prefecture People’s Hospital, Korla, 841000, Xinjiang, China | ||
| 2School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China | ||
| 3Department of General Surgery, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, 215009, Jiangsu, China | ||
| چکیده | ||
| Objective(s): To clarify therapeutic potential of albiflorin and its intrinsic mechanisms against dextran sulfate sodium (DSS)-induced Ulcerative colitis (UC) mice. Materials and Methods: Sixty male C57BL/6 mice were randomly divided into five groups: negative control, positive, albiflorin low-dose group, albiflorin high-dose group and treatment control (Salicylazosulfapyridine “SASP”, 100 mg/kg) group. Acute colitis was induced in all groups except NC by administration of 3% DSS for 7 days. Albiflorin and SASP were administered via the intragastric route twice a day for 7 days. The changes of colon tissue were assessed by disease activity index (DAI), HE staining, and ELISA. Adrenodoxin expressions of UC colon tissues were evaluated by immunohistochemistry. Western blotting was performed to investigate related protein of the NF-κB and MAPK signaling pathways. Results: It has been found that the albiflorin shares similar influences as the SASP in ameliorating the DSS-induced UC. The reduced DAI and alleviated colon tissue damage were observed in albiflorin intervened groups. Moreover, albiflorin significantly inhibited myeloperoxidase activities and attenuated immuno-inflammatory response and elevated Foxp3 mRNA in colon tissue. Furthermore, investigations revealed that albiflorin could inhibit adrenodoxin isoform and activate activated phosphorylated NF-κB p65 and IκBα, which consequently suppressed phosphorylated p38 mitogen-activated protein kinase (MAPK), extracellular regulated protein kinase (ERK), and c-Jun N-terminal kinase (JNK). Conclusion: These findings showed that albiflorin could alleviate DSS-induced murine colitis by activating inhibiting NF-κB and MAPK signaling pathways. It might be a potential therapeutic reagent for UC treatment. | ||
| کلیدواژهها | ||
| Albiflorin؛ Dextran sulfate sodium؛ MAPK؛ NF-κB؛ Ulcerative colitis | ||
| مراجع | ||
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