Enhanced antibacterial activity in skin exudates isolated MDR Staphylococcus aureus by γ-Al2O3 nanoparticles | ||
| Nanomedicine Journal | ||
| مقاله 4، دوره 10، شماره 1، فروردین 2023، صفحه 41-46 اصل مقاله (421.53 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22038/nmj.2022.68234.1732 | ||
| نویسندگان | ||
| Bahareh Panahi1؛ Mahkameh Soleiman Meygouni2؛ Davood Zaeifi* 1، 3 | ||
| 1Biology Department, Faculty of Biological Sciences, Tehran North Branch of Islamic Azad University, Tehran, Iran | ||
| 2Department of Biology, Islamic Azad University of Medical Sciences, Tehran, Iran | ||
| 3Department of Cell and Molecular Biology, School of Biology, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Objective(s): Staphylococcus aureus is one of the most common causes of infections affecting the skin and soft tissues, which causes many types of syndromes, including skin and soft tissue infections in humans. The quick occurrence of resistance to many antimicrobial substances and severe infections requires long-term intravenous administration of beta-lactamase-resistant Penicillin. Materials and Methods: The antimicrobial activity of γ-Al2O3 nanoparticles (NPs) against 20 clinical samples of S. aureus isolated from skin exudates compared with the standard ATCC 25923 strain investigated alone and in synergy with an antibiotic showed resistance. The most resistant isolates were selected based on being positive for MepA and Kirby and Bauer disc diffusion method. Minimum inhibitory concentration (MIC) of γ-Al2O3 NPs against S. aureus was determined within 0-360 min treatment time. Then, the double-disc synergy test (DDST) method was performed for semi-sensitive and antibiotic-resistant strains to evaluate the probable inhibitory effect in synergy form. Results: The selected isolate expressed the MepA gene, showed the highest susceptibility reaction against γ-Al2O3 NPs in Z=78.125 ml/μg-1 and Z=156.25 ml/μg-1, and the process continued by performing the best ratio of NPs on semi-sensitive and also resistance antibiotic in synergy with NPs for the bacteria strains. The synergy of γ-Al2O3 NPs and Tetracycline, Oxacillin, and Ceftazidime showed higher sensitivity compared to using antibiotics alone. Conclusion: The results of this study demonstrate that γ-Al2O3 has a strong antimicrobial effect and can enhance the properties and characteristics of antibacterial potency in synergy or developed synthetic functionalized NPs with antibiotics. | ||
| کلیدواژهها | ||
| MepA gene؛ Staphylococcus aureus؛ Skin exudates؛ γ-Al2O3 nanoparticles | ||
| مراجع | ||
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