Protective effects of equol on the cartilage and subchondral bone in ovariectomized rats with osteoarthritis | ||
| Iranian Journal of Basic Medical Sciences | ||
| مقاله 10، دوره 25، شماره 10، دی 2022، صفحه 1228-1233 اصل مقاله (430.32 K) | ||
| نوع مقاله: Original Article | ||
| شناسه دیجیتال (DOI): 10.22038/ijbms.2022.59036.13115 | ||
| نویسندگان | ||
| Shao-Hua Ping1؛ Fa-Ming Tian2؛ Ze-Ming Zhao1؛ Chun-Yu Liang1؛ Fang Liu1؛ Yu-Dan Wang2؛ Liu Zhang* 2، 3 | ||
| 1Department of Orthopedic Surgery, Affiliated Hospital of North China University of Science and Technology, Tangshan, 063000, China | ||
| 2School of Public Health, North China University of Science and Technology, Tangshan, 063000, China | ||
| 3Department of Orthopedic Surgery, Emergency General Hospital, Beijing, 100028, China | ||
| چکیده | ||
| Objective(s): This study aimed to determine the therapeutic effect of equol (EQ) on osteoporotic osteoarthritis (OP OA). Materials and Methods: Thirty-six 12-week-old female Sprague-Dawley rats were randomly divided into sham group, OP OA group, and EQ group (n=12). OP OA was induced by anterior cruciate ligament transection (ACLT) combined with ovariectomy (OVX). EQ was orally administrated (10 μg/g/day) after the operation for 12 weeks. The efficacy was evaluated by gross pathology and histopathologic evaluation. The underlying mechanism was investigated by immunohistochemical analysis, micro-computed tomography (micro-CT) scanning, and tartrate-resistant acid phosphatase (TRAP) staining. Results: EQ effectively retarded cartilage degeneration, decreased the levels of matrix metalloproteinases-13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS-5), nuclear factor-kappa B P65 (NF-κB P65) and caspase-3, and increased the levels of collagen type II (Col-II), Col-I, aggrecan (AGG), and inhibitor of NF-κB α (IκBα) in the cartilage. In addition, EQ increased bone mineral density, improved the microstructural parameters of the subchondral bone (SB), and decreased the number of osteoclasts. Conclusion: EQ exerted a chondroprotective effect on OP OA in rats, associated with inhibition of the NF-κB signaling pathway and chondrocyte apoptosis. Furthermore, EQ showed an osteoprotective effect on SB via inhibiting osteoclastic activities. | ||
| کلیدواژهها | ||
| Cartilage؛ Equol؛ Osteoarthritis؛ Ovariectomised rats؛ Subchondral bone | ||
| مراجع | ||
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