سامانه مدیریت نشریات علمی دانشگاه علوم پزشکی مشهد

Rahmani, Reihaneh, Darroudi, Majid, Gharanfoli, Mohsen, Chamani, Jamshidkhan, Gholamin, Mehran, Hashemi, Maryam. (1401). Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study. , 25(10), 1234-1242. doi: 10.22038/ijbms.2022.65590.14430
Reihaneh Rahmani; Majid Darroudi; Mohsen Gharanfoli; Jamshidkhan Chamani; Mehran Gholamin; Maryam Hashemi. "Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study". , 25, 10, 1401, 1234-1242. doi: 10.22038/ijbms.2022.65590.14430
Rahmani, Reihaneh, Darroudi, Majid, Gharanfoli, Mohsen, Chamani, Jamshidkhan, Gholamin, Mehran, Hashemi, Maryam. (1401). 'Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study', , 25(10), pp. 1234-1242. doi: 10.22038/ijbms.2022.65590.14430
Rahmani, Reihaneh, Darroudi, Majid, Gharanfoli, Mohsen, Chamani, Jamshidkhan, Gholamin, Mehran, Hashemi, Maryam. Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study. , 1401; 25(10): 1234-1242. doi: 10.22038/ijbms.2022.65590.14430

Conjugated PNC-27 peptide/PEI-Superparamagnetic iron oxide nanoparticles (SPIONs) as a double targeting agent for early cancer diagnosis: In vitro study

Iranian Journal of Basic Medical Sciences
مقاله 11، دوره 25، شماره 10، دی 2022، صفحه 1234-1242    اصل مقاله (880.95 K)
نوع مقاله: Original Article
شناسه دیجیتال (DOI): 10.22038/ijbms.2022.65590.14430
نویسندگان
Reihaneh Rahmani1، 2؛ Majid Darroudi3، 4؛ Mohsen Gharanfoli2، 5؛ Jamshidkhan Chamani6؛ Mehran Gholamin* 1، 7؛ Maryam Hashemi* 8، 9
1Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
2Research Institute of Applied Sciences (ACECR), Shahid Beheshti University, Tehran, Iran
3Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
4Department of Medical Biotechnology and Nanotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Cell and Molecular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
6Department of Biology, Faculty of Sciences, Mashhad Branch, Islamic Azad University, Mashhad, Iran
7Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
8Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
9Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
چکیده
Objective(s): Superparamagnetic iron oxide nanoparticles (SPIONs) have been considered promising non-invasive imaging tools in medicine. However, their high surface energy leads to NPs aggregation, while non-targeted SPIONs can cause cytotoxic effects on normal cells. In this work, we evaluated the in vitro potential of polyethyleneimine (PEI)-SPIONs targeted by PNC27 peptide as a double targeting agent throughout early cancer diagnosis.
Materials and Methods: Initially, PEI was conjugated to PNC27 with HDM-2-binding domain. Then, SPIONs were loaded into PEI-PNC27 through the ligand exchange method. The physicochemical characteristics of the synthesized NPs were evaluated. The cytotoxicity and targeting efficiency were assayed against HT-29 and CT-26 cell lines along with NIH-3t3 as normal cells by MTT method and Prussian blue staining test, respectively. 
Results: The mean diameter of synthesized carriers was obtained in the range of 86.6 – 116.1 nm with a positive charge. According to the cytotoxicity results, the binding and uptake abilities of the PNC27 peptide by cancer cells were significantly higher than that of the NIH-3t3 cells. However, the results were indicative of the more toxic impacts of targeted synthesized NPs against CT-26 cancer cell line when being compared with HT-29 cells, which may be caused by the different cytotoxicity mechanisms of NPs. In addition, the targeted carriers and SPIONs were present inside and around the cells with HDM-2 expression along with only a few non-targeted vectors, while displaying no appearance throughout the normal cell.
Conclusion: The results indicated the efficiency of targeted PEI-coated SPIONs for cancer diagnostic applications.
کلیدواژه‌ها
B-PEI؛ Cytotoxicity effect؛ Iron oxide؛ PNC27 peptide؛ SPIONs؛ Targeted cancer diagnostic
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